Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5688 and GFF5689 overlap by 25 nucleotides GFF5689 and GFF5690 overlap by 4 nucleotides GFF5690 and GFF5691 overlap by 4 nucleotides GFF5691 and GFF5692 are separated by 157 nucleotides
GFF5688 - Transcriptional regulator, LysR family, at 63,422 to 64,345
GFF5688
GFF5689 - Oxidoreductase, 2OG-Fe(II) oxygenase family, at 64,321 to 65,322
GFF5689
GFF5690 - Glutamate/aspartate ABC transporter, ATP-binding protein GltL (TC 3.A.1.3.4), at 65,319 to 66,062
GFF5690
GFF5691 - Glutamate/aspartate ABC transporter, permease protein GltK (TC 3.A.1.3.4), at 66,059 to 66,769
GFF5691
GFF5692 - Glutamate/aspartate ABC transporter, permease protein GltJ (TC 3.A.1.3.4), at 66,927 to 67,622
GFF5692
Position (kb)
65
66
67 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 64.376 kb on + strand at 64.376 kb on + strand at 64.376 kb on + strand at 64.376 kb on + strand at 64.376 kb on + strand at 64.377 kb on - strand at 64.377 kb on - strand at 64.377 kb on - strand at 64.572 kb on - strand, within GFF5689 at 64.572 kb on - strand, within GFF5689 at 64.572 kb on - strand, within GFF5689 at 64.778 kb on + strand, within GFF5689 at 64.779 kb on - strand, within GFF5689 at 64.817 kb on + strand, within GFF5689 at 64.818 kb on - strand, within GFF5689 at 64.833 kb on - strand, within GFF5689 at 64.907 kb on + strand, within GFF5689 at 64.907 kb on + strand, within GFF5689 at 64.907 kb on + strand, within GFF5689 at 64.908 kb on - strand, within GFF5689 at 64.908 kb on - strand, within GFF5689 at 65.018 kb on + strand, within GFF5689 at 65.018 kb on + strand, within GFF5689 at 65.019 kb on - strand, within GFF5689 at 65.019 kb on - strand, within GFF5689 at 65.019 kb on - strand, within GFF5689 at 65.207 kb on + strand, within GFF5689 at 65.207 kb on + strand, within GFF5689 at 65.207 kb on + strand, within GFF5689 at 65.207 kb on + strand, within GFF5689 at 65.208 kb on - strand, within GFF5689 at 65.208 kb on - strand, within GFF5689 at 65.208 kb on - strand, within GFF5689 at 65.208 kb on - strand, within GFF5689 at 65.208 kb on - strand, within GFF5689 at 65.446 kb on - strand, within GFF5690 at 65.446 kb on - strand, within GFF5690 at 66.023 kb on - strand at 66.023 kb on - strand at 66.031 kb on + strand at 66.031 kb on + strand at 66.123 kb on + strand at 66.124 kb on - strand at 66.159 kb on + strand, within GFF5691 at 66.159 kb on + strand, within GFF5691 at 66.159 kb on + strand, within GFF5691 at 66.159 kb on + strand, within GFF5691 at 66.160 kb on - strand, within GFF5691 at 66.160 kb on - strand, within GFF5691 at 66.160 kb on - strand, within GFF5691 at 66.173 kb on + strand, within GFF5691 at 66.180 kb on + strand, within GFF5691 at 66.180 kb on + strand, within GFF5691 at 66.180 kb on + strand, within GFF5691 at 66.180 kb on + strand, within GFF5691 at 66.180 kb on + strand, within GFF5691 at 66.181 kb on - strand, within GFF5691 at 66.181 kb on - strand, within GFF5691 at 66.181 kb on - strand, within GFF5691 at 66.181 kb on - strand, within GFF5691 at 66.181 kb on - strand, within GFF5691 at 66.181 kb on - strand, within GFF5691 at 66.258 kb on + strand, within GFF5691 at 66.258 kb on + strand, within GFF5691 at 66.258 kb on + strand, within GFF5691 at 66.258 kb on + strand, within GFF5691 at 66.258 kb on + strand, within GFF5691 at 66.259 kb on - strand, within GFF5691 at 66.259 kb on - strand, within GFF5691 at 66.259 kb on - strand, within GFF5691 at 66.259 kb on - strand, within GFF5691 at 66.364 kb on - strand, within GFF5691 at 66.528 kb on + strand, within GFF5691 at 66.528 kb on + strand, within GFF5691 at 66.529 kb on - strand, within GFF5691 at 66.529 kb on - strand, within GFF5691 at 66.529 kb on - strand, within GFF5691 at 66.738 kb on + strand at 66.738 kb on + strand at 66.738 kb on + strand at 66.738 kb on + strand at 66.739 kb on - strand at 66.747 kb on + strand at 66.958 kb on + strand at 66.958 kb on + strand at 66.958 kb on + strand at 66.959 kb on - strand at 66.959 kb on - strand at 67.000 kb on + strand, within GFF5692 at 67.000 kb on + strand, within GFF5692 at 67.000 kb on + strand, within GFF5692 at 67.001 kb on - strand, within GFF5692 at 67.001 kb on - strand, within GFF5692 at 67.001 kb on - strand, within GFF5692 at 67.001 kb on - strand, within GFF5692
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 64,376 + +0.5 64,376 + +0.0 64,376 + -1.3 64,376 + +2.6 64,376 + +0.2 64,377 - +0.5 64,377 - +2.0 64,377 - -0.2 64,572 - GFF5689 0.25 +1.2 64,572 - GFF5689 0.25 +0.0 64,572 - GFF5689 0.25 -0.5 64,778 + GFF5689 0.46 +1.2 64,779 - GFF5689 0.46 +0.1 64,817 + GFF5689 0.50 +0.5 64,818 - GFF5689 0.50 -0.4 64,833 - GFF5689 0.51 -0.7 64,907 + GFF5689 0.58 +0.6 64,907 + GFF5689 0.58 +1.6 64,907 + GFF5689 0.58 -0.6 64,908 - GFF5689 0.59 -0.2 64,908 - GFF5689 0.59 -0.2 65,018 + GFF5689 0.70 -0.8 65,018 + GFF5689 0.70 +0.1 65,019 - GFF5689 0.70 -1.5 65,019 - GFF5689 0.70 +0.5 65,019 - GFF5689 0.70 -0.3 65,207 + GFF5689 0.88 +0.2 65,207 + GFF5689 0.88 +0.7 65,207 + GFF5689 0.88 +1.2 65,207 + GFF5689 0.88 +1.4 65,208 - GFF5689 0.89 -0.8 65,208 - GFF5689 0.89 +0.1 65,208 - GFF5689 0.89 -0.9 65,208 - GFF5689 0.89 +0.6 65,208 - GFF5689 0.89 -0.1 65,446 - GFF5690 0.17 -0.3 65,446 - GFF5690 0.17 +0.3 66,023 - -0.7 66,023 - -0.3 66,031 + +0.2 66,031 + -1.6 66,123 + +0.1 66,124 - +0.3 66,159 + GFF5691 0.14 -0.5 66,159 + GFF5691 0.14 +0.5 66,159 + GFF5691 0.14 -0.5 66,159 + GFF5691 0.14 -0.9 66,160 - GFF5691 0.14 -0.4 66,160 - GFF5691 0.14 -1.2 66,160 - GFF5691 0.14 +0.6 66,173 + GFF5691 0.16 +0.8 66,180 + GFF5691 0.17 -0.8 66,180 + GFF5691 0.17 -0.9 66,180 + GFF5691 0.17 -1.4 66,180 + GFF5691 0.17 -2.1 66,180 + GFF5691 0.17 -0.3 66,181 - GFF5691 0.17 -0.1 66,181 - GFF5691 0.17 -0.4 66,181 - GFF5691 0.17 -1.3 66,181 - GFF5691 0.17 -0.3 66,181 - GFF5691 0.17 -0.3 66,181 - GFF5691 0.17 -0.5 66,258 + GFF5691 0.28 +1.1 66,258 + GFF5691 0.28 -0.4 66,258 + GFF5691 0.28 -0.6 66,258 + GFF5691 0.28 +0.8 66,258 + GFF5691 0.28 +0.5 66,259 - GFF5691 0.28 -3.5 66,259 - GFF5691 0.28 +0.3 66,259 - GFF5691 0.28 -0.7 66,259 - GFF5691 0.28 -0.2 66,364 - GFF5691 0.43 -0.2 66,528 + GFF5691 0.66 -0.3 66,528 + GFF5691 0.66 -0.2 66,529 - GFF5691 0.66 -0.4 66,529 - GFF5691 0.66 -2.1 66,529 - GFF5691 0.66 +0.1 66,738 + -1.2 66,738 + +1.2 66,738 + -0.8 66,738 + -0.5 66,739 - +1.2 66,747 + +1.3 66,958 + +0.1 66,958 + -0.7 66,958 + -0.4 66,959 - +0.2 66,959 - -0.3 67,000 + GFF5692 0.10 -0.0 67,000 + GFF5692 0.10 -1.1 67,000 + GFF5692 0.10 -0.1 67,001 - GFF5692 0.11 -0.1 67,001 - GFF5692 0.11 +0.1 67,001 - GFF5692 0.11 +1.1 67,001 - GFF5692 0.11 -1.3
Or see this region's nucleotide sequence