Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5538 and GFF5539 are separated by 6 nucleotides GFF5539 and GFF5540 are separated by 114 nucleotides GFF5540 and GFF5541 are separated by 15 nucleotides
GFF5538 - ATP-dependent DNA helicase RecG (EC 3.6.4.12), at 167,063 to 169,195
GFF5538
GFF5539 - Transporter, LysE family, at 169,202 to 169,834
GFF5539
GFF5540 - FIG01201438: hypothetical protein, at 169,949 to 170,389
GFF5540
GFF5541 - Transcriptional regulator, GntR family, at 170,405 to 171,109
GFF5541
Position (kb)
169
170 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 168.386 kb on - strand, within GFF5538 at 168.386 kb on - strand, within GFF5538 at 168.386 kb on - strand, within GFF5538 at 168.386 kb on - strand, within GFF5538 at 168.386 kb on - strand, within GFF5538 at 168.416 kb on + strand, within GFF5538 at 168.416 kb on + strand, within GFF5538 at 168.416 kb on + strand, within GFF5538 at 168.416 kb on + strand, within GFF5538 at 168.416 kb on + strand, within GFF5538 at 168.416 kb on + strand, within GFF5538 at 168.416 kb on + strand, within GFF5538 at 168.416 kb on + strand, within GFF5538 at 168.416 kb on + strand, within GFF5538 at 168.416 kb on + strand, within GFF5538 at 168.417 kb on - strand, within GFF5538 at 168.417 kb on - strand, within GFF5538 at 168.417 kb on - strand, within GFF5538 at 168.417 kb on - strand, within GFF5538 at 168.417 kb on - strand, within GFF5538 at 168.417 kb on - strand, within GFF5538 at 168.417 kb on - strand, within GFF5538 at 168.419 kb on + strand, within GFF5538 at 168.419 kb on + strand, within GFF5538 at 168.420 kb on - strand, within GFF5538 at 168.420 kb on - strand, within GFF5538 at 168.420 kb on - strand, within GFF5538 at 168.557 kb on + strand, within GFF5538 at 168.558 kb on - strand, within GFF5538 at 168.558 kb on - strand, within GFF5538 at 168.558 kb on - strand, within GFF5538 at 168.761 kb on + strand, within GFF5538 at 168.893 kb on + strand, within GFF5538 at 168.893 kb on + strand, within GFF5538 at 168.893 kb on + strand, within GFF5538 at 168.893 kb on + strand, within GFF5538 at 168.893 kb on + strand, within GFF5538 at 169.181 kb on + strand at 169.181 kb on + strand at 169.335 kb on + strand, within GFF5539 at 169.335 kb on + strand, within GFF5539 at 169.335 kb on + strand, within GFF5539 at 169.335 kb on + strand, within GFF5539 at 169.335 kb on + strand, within GFF5539 at 169.335 kb on + strand, within GFF5539 at 169.336 kb on - strand, within GFF5539 at 169.336 kb on - strand, within GFF5539 at 169.336 kb on - strand, within GFF5539 at 169.336 kb on - strand, within GFF5539 at 169.336 kb on - strand, within GFF5539 at 169.389 kb on + strand, within GFF5539 at 169.389 kb on + strand, within GFF5539 at 169.389 kb on + strand, within GFF5539 at 169.389 kb on + strand, within GFF5539 at 169.389 kb on + strand, within GFF5539 at 169.389 kb on + strand, within GFF5539 at 169.389 kb on + strand, within GFF5539 at 169.390 kb on - strand, within GFF5539 at 169.390 kb on - strand, within GFF5539 at 169.390 kb on - strand, within GFF5539 at 169.390 kb on - strand, within GFF5539 at 169.390 kb on - strand, within GFF5539 at 169.401 kb on + strand, within GFF5539 at 169.590 kb on + strand, within GFF5539 at 169.590 kb on + strand, within GFF5539 at 169.591 kb on - strand, within GFF5539 at 169.591 kb on - strand, within GFF5539 at 169.591 kb on - strand, within GFF5539 at 169.605 kb on + strand, within GFF5539 at 169.605 kb on + strand, within GFF5539 at 169.605 kb on + strand, within GFF5539 at 169.605 kb on + strand, within GFF5539 at 169.605 kb on + strand, within GFF5539 at 169.605 kb on + strand, within GFF5539 at 169.605 kb on + strand, within GFF5539 at 169.605 kb on + strand, within GFF5539 at 169.605 kb on + strand, within GFF5539 at 169.605 kb on + strand, within GFF5539 at 169.605 kb on + strand, within GFF5539 at 169.605 kb on + strand, within GFF5539 at 169.606 kb on - strand, within GFF5539 at 169.606 kb on - strand, within GFF5539 at 169.606 kb on - strand, within GFF5539 at 169.606 kb on - strand, within GFF5539 at 169.606 kb on - strand, within GFF5539 at 169.818 kb on + strand at 169.818 kb on + strand at 169.819 kb on - strand at 169.819 kb on - strand at 169.819 kb on - strand at 169.839 kb on + strand at 169.839 kb on + strand at 169.839 kb on + strand at 169.871 kb on + strand at 169.871 kb on + strand at 169.872 kb on - strand at 169.874 kb on - strand at 170.049 kb on - strand, within GFF5540 at 170.049 kb on - strand, within GFF5540 at 170.049 kb on - strand, within GFF5540 at 170.049 kb on - strand, within GFF5540 at 170.049 kb on - strand, within GFF5540 at 170.333 kb on + strand, within GFF5540 at 170.333 kb on + strand, within GFF5540 at 170.333 kb on + strand, within GFF5540 at 170.334 kb on - strand, within GFF5540 at 170.334 kb on - strand, within GFF5540 at 170.334 kb on - strand, within GFF5540 at 170.334 kb on - strand, within GFF5540 at 170.334 kb on - strand, within GFF5540 at 170.334 kb on - strand, within GFF5540 at 170.334 kb on - strand, within GFF5540 at 170.442 kb on + strand at 170.442 kb on + strand at 170.443 kb on - strand at 170.443 kb on - strand at 170.443 kb on - strand at 170.443 kb on - strand at 170.443 kb on - strand at 170.443 kb on - strand at 170.478 kb on + strand, within GFF5541 at 170.478 kb on + strand, within GFF5541 at 170.478 kb on + strand, within GFF5541 at 170.478 kb on + strand, within GFF5541 at 170.479 kb on - strand, within GFF5541 at 170.479 kb on - strand, within GFF5541 at 170.479 kb on - strand, within GFF5541 at 170.479 kb on - strand, within GFF5541 at 170.479 kb on - strand, within GFF5541 at 170.479 kb on - strand, within GFF5541 at 170.556 kb on + strand, within GFF5541 at 170.556 kb on + strand, within GFF5541 at 170.557 kb on - strand, within GFF5541 at 170.784 kb on + strand, within GFF5541 at 170.784 kb on + strand, within GFF5541 at 170.785 kb on - strand, within GFF5541 at 170.829 kb on + strand, within GFF5541 at 170.829 kb on + strand, within GFF5541 at 170.829 kb on + strand, within GFF5541 at 170.829 kb on + strand, within GFF5541 at 170.829 kb on + strand, within GFF5541 at 170.829 kb on + strand, within GFF5541 at 170.830 kb on - strand, within GFF5541 at 170.830 kb on - strand, within GFF5541 at 170.830 kb on - strand, within GFF5541 at 170.830 kb on - strand, within GFF5541 at 170.830 kb on - strand, within GFF5541 at 170.830 kb on - strand, within GFF5541 at 170.830 kb on - strand, within GFF5541 at 170.830 kb on - strand, within GFF5541 at 170.830 kb on - strand, within GFF5541 at 170.830 kb on - strand, within GFF5541 at 170.830 kb on - strand, within GFF5541 at 170.830 kb on - strand, within GFF5541 at 170.830 kb on - strand, within GFF5541
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 168,386 - GFF5538 0.62 -1.4 168,386 - GFF5538 0.62 +0.1 168,386 - GFF5538 0.62 -0.9 168,386 - GFF5538 0.62 -0.9 168,386 - GFF5538 0.62 +0.4 168,416 + GFF5538 0.63 -0.2 168,416 + GFF5538 0.63 -1.3 168,416 + GFF5538 0.63 +0.6 168,416 + GFF5538 0.63 -1.3 168,416 + GFF5538 0.63 -0.2 168,416 + GFF5538 0.63 -0.4 168,416 + GFF5538 0.63 +0.5 168,416 + GFF5538 0.63 +0.0 168,416 + GFF5538 0.63 -0.6 168,416 + GFF5538 0.63 -0.7 168,417 - GFF5538 0.63 +0.5 168,417 - GFF5538 0.63 -0.8 168,417 - GFF5538 0.63 +0.4 168,417 - GFF5538 0.63 -0.1 168,417 - GFF5538 0.63 +0.3 168,417 - GFF5538 0.63 +0.7 168,417 - GFF5538 0.63 -0.8 168,419 + GFF5538 0.64 +0.3 168,419 + GFF5538 0.64 -0.4 168,420 - GFF5538 0.64 +0.3 168,420 - GFF5538 0.64 -0.3 168,420 - GFF5538 0.64 -0.5 168,557 + GFF5538 0.70 -0.5 168,558 - GFF5538 0.70 +0.1 168,558 - GFF5538 0.70 -1.0 168,558 - GFF5538 0.70 -0.3 168,761 + GFF5538 0.80 -1.3 168,893 + GFF5538 0.86 -0.1 168,893 + GFF5538 0.86 -0.7 168,893 + GFF5538 0.86 -0.4 168,893 + GFF5538 0.86 -0.7 168,893 + GFF5538 0.86 -0.4 169,181 + -0.3 169,181 + +1.2 169,335 + GFF5539 0.21 +0.1 169,335 + GFF5539 0.21 +0.5 169,335 + GFF5539 0.21 +0.5 169,335 + GFF5539 0.21 -0.1 169,335 + GFF5539 0.21 -1.6 169,335 + GFF5539 0.21 +0.3 169,336 - GFF5539 0.21 +0.2 169,336 - GFF5539 0.21 +2.3 169,336 - GFF5539 0.21 +0.1 169,336 - GFF5539 0.21 -0.1 169,336 - GFF5539 0.21 +0.3 169,389 + GFF5539 0.30 +0.7 169,389 + GFF5539 0.30 -0.7 169,389 + GFF5539 0.30 +0.2 169,389 + GFF5539 0.30 +3.1 169,389 + GFF5539 0.30 +0.1 169,389 + GFF5539 0.30 +0.1 169,389 + GFF5539 0.30 +1.2 169,390 - GFF5539 0.30 -0.0 169,390 - GFF5539 0.30 -2.6 169,390 - GFF5539 0.30 +0.1 169,390 - GFF5539 0.30 -0.6 169,390 - GFF5539 0.30 +0.2 169,401 + GFF5539 0.31 -0.3 169,590 + GFF5539 0.61 +0.4 169,590 + GFF5539 0.61 +0.4 169,591 - GFF5539 0.61 +1.4 169,591 - GFF5539 0.61 -0.3 169,591 - GFF5539 0.61 +0.3 169,605 + GFF5539 0.64 -2.0 169,605 + GFF5539 0.64 -0.0 169,605 + GFF5539 0.64 -0.1 169,605 + GFF5539 0.64 +0.7 169,605 + GFF5539 0.64 +1.0 169,605 + GFF5539 0.64 +0.6 169,605 + GFF5539 0.64 -1.8 169,605 + GFF5539 0.64 -0.3 169,605 + GFF5539 0.64 -0.3 169,605 + GFF5539 0.64 -1.6 169,605 + GFF5539 0.64 -0.4 169,605 + GFF5539 0.64 -0.2 169,606 - GFF5539 0.64 +1.3 169,606 - GFF5539 0.64 +0.7 169,606 - GFF5539 0.64 -0.1 169,606 - GFF5539 0.64 -0.4 169,606 - GFF5539 0.64 -1.0 169,818 + -0.8 169,818 + +0.7 169,819 - +0.1 169,819 - +0.0 169,819 - -0.3 169,839 + -0.8 169,839 + -0.0 169,839 + +0.0 169,871 + -1.5 169,871 + +0.1 169,872 - -0.6 169,874 - -0.0 170,049 - GFF5540 0.23 -0.7 170,049 - GFF5540 0.23 -0.6 170,049 - GFF5540 0.23 -0.1 170,049 - GFF5540 0.23 +0.1 170,049 - GFF5540 0.23 +0.1 170,333 + GFF5540 0.87 -0.0 170,333 + GFF5540 0.87 -3.0 170,333 + GFF5540 0.87 -0.5 170,334 - GFF5540 0.87 -0.0 170,334 - GFF5540 0.87 -0.5 170,334 - GFF5540 0.87 +0.3 170,334 - GFF5540 0.87 -1.8 170,334 - GFF5540 0.87 -0.7 170,334 - GFF5540 0.87 -1.5 170,334 - GFF5540 0.87 -0.0 170,442 + +0.4 170,442 + +1.2 170,443 - +0.9 170,443 - -0.3 170,443 - -0.8 170,443 - -0.7 170,443 - -0.7 170,443 - +0.7 170,478 + GFF5541 0.10 -1.6 170,478 + GFF5541 0.10 +0.9 170,478 + GFF5541 0.10 +0.7 170,478 + GFF5541 0.10 -0.3 170,479 - GFF5541 0.10 -1.5 170,479 - GFF5541 0.10 +0.5 170,479 - GFF5541 0.10 +0.3 170,479 - GFF5541 0.10 +0.4 170,479 - GFF5541 0.10 +1.7 170,479 - GFF5541 0.10 +2.2 170,556 + GFF5541 0.21 +0.4 170,556 + GFF5541 0.21 +0.5 170,557 - GFF5541 0.22 -0.3 170,784 + GFF5541 0.54 +0.5 170,784 + GFF5541 0.54 -2.3 170,785 - GFF5541 0.54 +1.3 170,829 + GFF5541 0.60 -0.3 170,829 + GFF5541 0.60 +0.7 170,829 + GFF5541 0.60 -0.3 170,829 + GFF5541 0.60 -0.6 170,829 + GFF5541 0.60 -2.0 170,829 + GFF5541 0.60 -0.7 170,830 - GFF5541 0.60 +1.1 170,830 - GFF5541 0.60 -0.1 170,830 - GFF5541 0.60 -0.6 170,830 - GFF5541 0.60 -0.2 170,830 - GFF5541 0.60 -0.1 170,830 - GFF5541 0.60 -0.4 170,830 - GFF5541 0.60 +1.2 170,830 - GFF5541 0.60 +0.4 170,830 - GFF5541 0.60 -1.3 170,830 - GFF5541 0.60 +0.2 170,830 - GFF5541 0.60 -0.6 170,830 - GFF5541 0.60 +0.5 170,830 - GFF5541 0.60 +1.0
Or see this region's nucleotide sequence