Strain Fitness in Variovorax sp. SCN45 around GFF5374

Experiment: M9 with Supernatant from Cyptococcus

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5373 and GFF5374 are separated by 86 nucleotidesGFF5374 and GFF5375 are separated by 21 nucleotides GFF5373 - Xylose isomerase-like TIM-barrel protein KPN_00539, at 45 to 452 GFF5373 GFF5374 - Glutamine amidotransferase, class I, at 539 to 1,483 GFF5374 GFF5375 - Acyl-CoA dehydrogenase, at 1,505 to 3,145 GFF5375 Position (kb) 0 1 2Strain fitness (log2 ratio) -3 -2 -1 0 1at 0.046 kb on + strandat 0.046 kb on + strandat 0.046 kb on + strandat 0.046 kb on + strandat 0.088 kb on + strand, within GFF5373at 0.088 kb on + strand, within GFF5373at 0.088 kb on + strand, within GFF5373at 0.175 kb on + strand, within GFF5373at 0.175 kb on + strand, within GFF5373at 0.175 kb on + strand, within GFF5373at 0.176 kb on - strand, within GFF5373at 0.176 kb on - strand, within GFF5373at 0.176 kb on - strand, within GFF5373at 0.274 kb on + strand, within GFF5373at 0.274 kb on + strand, within GFF5373at 0.274 kb on + strand, within GFF5373at 0.274 kb on + strand, within GFF5373at 0.274 kb on + strand, within GFF5373at 0.274 kb on + strand, within GFF5373at 0.275 kb on - strand, within GFF5373at 0.275 kb on - strand, within GFF5373at 0.275 kb on - strand, within GFF5373at 0.275 kb on - strand, within GFF5373at 0.275 kb on - strand, within GFF5373at 0.275 kb on - strand, within GFF5373at 0.275 kb on - strand, within GFF5373at 0.275 kb on - strand, within GFF5373at 0.275 kb on - strand, within GFF5373at 0.275 kb on - strand, within GFF5373at 0.275 kb on - strand, within GFF5373at 0.346 kb on + strand, within GFF5373at 0.346 kb on + strand, within GFF5373at 0.346 kb on + strand, within GFF5373at 0.346 kb on + strand, within GFF5373at 0.346 kb on + strand, within GFF5373at 0.346 kb on + strand, within GFF5373at 0.346 kb on + strand, within GFF5373at 0.347 kb on - strand, within GFF5373at 0.347 kb on - strand, within GFF5373at 0.347 kb on - strand, within GFF5373at 0.347 kb on - strand, within GFF5373at 0.347 kb on - strand, within GFF5373at 0.493 kb on + strandat 0.493 kb on + strandat 0.493 kb on + strandat 0.493 kb on + strandat 0.493 kb on + strandat 0.493 kb on + strandat 0.493 kb on + strandat 0.493 kb on + strandat 0.493 kb on + strandat 0.494 kb on - strandat 0.494 kb on - strandat 0.494 kb on - strandat 0.494 kb on - strandat 0.494 kb on - strandat 0.494 kb on - strandat 0.494 kb on - strandat 0.692 kb on + strand, within GFF5374at 0.692 kb on + strand, within GFF5374at 0.692 kb on + strand, within GFF5374at 0.692 kb on + strand, within GFF5374at 0.692 kb on + strand, within GFF5374at 0.692 kb on + strand, within GFF5374at 0.692 kb on + strand, within GFF5374at 0.692 kb on + strand, within GFF5374at 0.692 kb on + strand, within GFF5374at 0.693 kb on - strand, within GFF5374at 0.693 kb on - strand, within GFF5374at 0.693 kb on - strand, within GFF5374at 0.693 kb on - strand, within GFF5374at 0.800 kb on + strand, within GFF5374at 0.800 kb on + strand, within GFF5374at 0.801 kb on - strand, within GFF5374at 0.801 kb on - strand, within GFF5374at 0.860 kb on + strand, within GFF5374at 0.860 kb on + strand, within GFF5374at 0.860 kb on + strand, within GFF5374at 0.860 kb on + strand, within GFF5374at 0.861 kb on - strand, within GFF5374at 0.861 kb on - strand, within GFF5374at 0.861 kb on - strand, within GFF5374at 0.861 kb on - strand, within GFF5374at 0.861 kb on - strand, within GFF5374at 0.861 kb on - strand, within GFF5374at 0.861 kb on - strand, within GFF5374at 0.914 kb on + strand, within GFF5374at 0.914 kb on + strand, within GFF5374at 0.915 kb on - strand, within GFF5374at 0.915 kb on - strand, within GFF5374at 0.915 kb on - strand, within GFF5374at 0.915 kb on - strand, within GFF5374at 0.915 kb on - strand, within GFF5374at 1.016 kb on + strand, within GFF5374at 1.016 kb on + strand, within GFF5374at 1.016 kb on + strand, within GFF5374at 1.016 kb on + strand, within GFF5374at 1.016 kb on + strand, within GFF5374at 1.016 kb on + strand, within GFF5374at 1.017 kb on - strand, within GFF5374at 1.017 kb on - strand, within GFF5374at 1.079 kb on + strand, within GFF5374at 1.079 kb on + strand, within GFF5374at 1.079 kb on + strand, within GFF5374at 1.079 kb on + strand, within GFF5374at 1.079 kb on + strand, within GFF5374at 1.079 kb on + strand, within GFF5374at 1.079 kb on + strand, within GFF5374at 1.079 kb on + strand, within GFF5374at 1.080 kb on - strand, within GFF5374at 1.080 kb on - strand, within GFF5374at 1.080 kb on - strand, within GFF5374at 1.080 kb on - strand, within GFF5374at 1.080 kb on - strand, within GFF5374at 1.080 kb on - strand, within GFF5374at 1.137 kb on - strand, within GFF5374at 1.137 kb on - strand, within GFF5374at 1.137 kb on - strand, within GFF5374at 1.137 kb on - strand, within GFF5374at 1.280 kb on + strand, within GFF5374at 1.280 kb on + strand, within GFF5374at 1.280 kb on + strand, within GFF5374at 1.280 kb on + strand, within GFF5374at 1.280 kb on + strand, within GFF5374at 1.280 kb on + strand, within GFF5374at 1.280 kb on + strand, within GFF5374at 1.280 kb on + strand, within GFF5374at 1.280 kb on + strand, within GFF5374at 1.280 kb on + strand, within GFF5374at 1.281 kb on - strand, within GFF5374at 1.281 kb on - strand, within GFF5374at 1.281 kb on - strand, within GFF5374at 1.281 kb on - strand, within GFF5374at 1.281 kb on - strand, within GFF5374at 1.319 kb on + strand, within GFF5374at 1.319 kb on + strand, within GFF5374at 1.319 kb on + strand, within GFF5374at 1.319 kb on + strand, within GFF5374at 1.319 kb on + strand, within GFF5374at 1.320 kb on - strand, within GFF5374at 1.320 kb on - strand, within GFF5374at 1.320 kb on - strand, within GFF5374at 1.320 kb on - strand, within GFF5374at 1.320 kb on - strand, within GFF5374at 1.926 kb on + strand, within GFF5375at 1.926 kb on + strand, within GFF5375at 1.926 kb on + strand, within GFF5375at 1.926 kb on + strand, within GFF5375at 1.926 kb on + strand, within GFF5375at 1.926 kb on + strand, within GFF5375at 1.926 kb on + strand, within GFF5375at 1.927 kb on - strand, within GFF5375at 1.927 kb on - strand, within GFF5375at 1.927 kb on - strand, within GFF5375at 1.927 kb on - strand, within GFF5375at 1.927 kb on - strand, within GFF5375at 2.178 kb on + strand, within GFF5375at 2.178 kb on + strand, within GFF5375at 2.178 kb on + strand, within GFF5375at 2.178 kb on + strand, within GFF5375at 2.178 kb on + strand, within GFF5375at 2.179 kb on - strand, within GFF5375at 2.179 kb on - strand, within GFF5375

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus
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46 + -0.3
46 + -0.7
46 + -0.6
46 + -0.7
88 + GFF5373 0.11 -1.4
88 + GFF5373 0.11 -0.1
88 + GFF5373 0.11 -0.1
175 + GFF5373 0.32 +0.4
175 + GFF5373 0.32 -0.3
175 + GFF5373 0.32 -0.2
176 - GFF5373 0.32 +0.2
176 - GFF5373 0.32 -0.4
176 - GFF5373 0.32 +0.6
274 + GFF5373 0.56 -0.4
274 + GFF5373 0.56 -0.5
274 + GFF5373 0.56 +0.7
274 + GFF5373 0.56 +0.5
274 + GFF5373 0.56 -0.5
274 + GFF5373 0.56 -0.1
275 - GFF5373 0.56 +0.2
275 - GFF5373 0.56 -0.0
275 - GFF5373 0.56 -0.8
275 - GFF5373 0.56 -0.1
275 - GFF5373 0.56 +1.5
275 - GFF5373 0.56 +0.2
275 - GFF5373 0.56 -0.2
275 - GFF5373 0.56 +0.5
275 - GFF5373 0.56 -0.3
275 - GFF5373 0.56 -0.2
275 - GFF5373 0.56 +0.0
346 + GFF5373 0.74 -2.0
346 + GFF5373 0.74 +0.9
346 + GFF5373 0.74 +1.1
346 + GFF5373 0.74 -0.4
346 + GFF5373 0.74 -1.4
346 + GFF5373 0.74 -1.0
346 + GFF5373 0.74 +0.3
347 - GFF5373 0.74 +0.0
347 - GFF5373 0.74 +0.6
347 - GFF5373 0.74 +1.7
347 - GFF5373 0.74 -0.2
347 - GFF5373 0.74 +0.0
493 + -1.0
493 + +0.9
493 + +0.0
493 + -1.4
493 + -0.9
493 + -0.7
493 + +1.0
493 + +0.9
493 + -0.6
494 - -0.4
494 - +0.5
494 - +1.6
494 - -0.2
494 - -2.0
494 - +0.6
494 - +0.2
692 + GFF5374 0.16 -0.4
692 + GFF5374 0.16 +0.5
692 + GFF5374 0.16 +0.5
692 + GFF5374 0.16 -0.2
692 + GFF5374 0.16 +0.1
692 + GFF5374 0.16 -0.5
692 + GFF5374 0.16 +0.9
692 + GFF5374 0.16 -0.8
692 + GFF5374 0.16 -0.2
693 - GFF5374 0.16 +1.3
693 - GFF5374 0.16 +1.2
693 - GFF5374 0.16 -1.1
693 - GFF5374 0.16 -0.1
800 + GFF5374 0.28 -0.2
800 + GFF5374 0.28 -0.1
801 - GFF5374 0.28 +0.2
801 - GFF5374 0.28 -0.8
860 + GFF5374 0.34 +0.6
860 + GFF5374 0.34 -0.6
860 + GFF5374 0.34 -0.3
860 + GFF5374 0.34 -0.8
861 - GFF5374 0.34 -0.1
861 - GFF5374 0.34 +1.7
861 - GFF5374 0.34 +0.1
861 - GFF5374 0.34 +0.0
861 - GFF5374 0.34 +1.1
861 - GFF5374 0.34 -3.0
861 - GFF5374 0.34 -0.8
914 + GFF5374 0.40 -0.5
914 + GFF5374 0.40 -0.3
915 - GFF5374 0.40 -0.5
915 - GFF5374 0.40 -0.8
915 - GFF5374 0.40 +0.6
915 - GFF5374 0.40 -1.2
915 - GFF5374 0.40 +0.6
1,016 + GFF5374 0.50 -0.1
1,016 + GFF5374 0.50 -0.2
1,016 + GFF5374 0.50 +0.4
1,016 + GFF5374 0.50 -0.9
1,016 + GFF5374 0.50 -1.2
1,016 + GFF5374 0.50 +0.8
1,017 - GFF5374 0.51 -0.1
1,017 - GFF5374 0.51 +0.2
1,079 + GFF5374 0.57 +0.4
1,079 + GFF5374 0.57 +0.7
1,079 + GFF5374 0.57 +0.8
1,079 + GFF5374 0.57 -1.0
1,079 + GFF5374 0.57 -1.1
1,079 + GFF5374 0.57 +0.7
1,079 + GFF5374 0.57 +0.0
1,079 + GFF5374 0.57 +0.5
1,080 - GFF5374 0.57 -1.7
1,080 - GFF5374 0.57 +0.6
1,080 - GFF5374 0.57 +0.5
1,080 - GFF5374 0.57 -0.1
1,080 - GFF5374 0.57 -0.8
1,080 - GFF5374 0.57 -0.8
1,137 - GFF5374 0.63 -0.6
1,137 - GFF5374 0.63 -0.5
1,137 - GFF5374 0.63 -0.6
1,137 - GFF5374 0.63 -1.3
1,280 + GFF5374 0.78 +1.0
1,280 + GFF5374 0.78 +0.4
1,280 + GFF5374 0.78 -0.3
1,280 + GFF5374 0.78 -0.4
1,280 + GFF5374 0.78 +0.1
1,280 + GFF5374 0.78 +0.1
1,280 + GFF5374 0.78 +0.4
1,280 + GFF5374 0.78 +0.2
1,280 + GFF5374 0.78 +1.4
1,280 + GFF5374 0.78 +1.2
1,281 - GFF5374 0.79 -0.3
1,281 - GFF5374 0.79 -0.8
1,281 - GFF5374 0.79 +1.0
1,281 - GFF5374 0.79 -1.6
1,281 - GFF5374 0.79 -0.6
1,319 + GFF5374 0.83 -0.5
1,319 + GFF5374 0.83 -0.8
1,319 + GFF5374 0.83 -0.6
1,319 + GFF5374 0.83 -1.8
1,319 + GFF5374 0.83 -0.3
1,320 - GFF5374 0.83 +0.4
1,320 - GFF5374 0.83 +0.6
1,320 - GFF5374 0.83 +0.3
1,320 - GFF5374 0.83 +0.5
1,320 - GFF5374 0.83 -1.0
1,926 + GFF5375 0.26 -0.6
1,926 + GFF5375 0.26 +0.2
1,926 + GFF5375 0.26 +1.3
1,926 + GFF5375 0.26 +0.0
1,926 + GFF5375 0.26 -1.3
1,926 + GFF5375 0.26 +0.6
1,926 + GFF5375 0.26 -0.7
1,927 - GFF5375 0.26 -0.8
1,927 - GFF5375 0.26 -0.3
1,927 - GFF5375 0.26 +0.9
1,927 - GFF5375 0.26 -0.5
1,927 - GFF5375 0.26 -1.0
2,178 + GFF5375 0.41 -0.8
2,178 + GFF5375 0.41 -0.4
2,178 + GFF5375 0.41 -2.1
2,178 + GFF5375 0.41 +0.2
2,178 + GFF5375 0.41 +0.4
2,179 - GFF5375 0.41 +0.7
2,179 - GFF5375 0.41 +0.6

Or see this region's nucleotide sequence