Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5302 and GFF5303 are separated by 174 nucleotides GFF5303 and GFF5304 are separated by 1 nucleotides GFF5304 and GFF5305 are separated by 21 nucleotides
GFF5302 - ABC transporter, substrate-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 177,712 to 178,752
GFF5302
GFF5303 - Tricarboxylate transport transcriptional regulator TctD, at 178,927 to 179,601
GFF5303
GFF5304 - no description, at 179,603 to 181,039
GFF5304
GFF5305 - S-adenosylmethionine synthetase (EC 2.5.1.6), at 181,061 to 182,248
GFF5305
Position (kb) 179
180
181
182 Strain fitness (log2 ratio) -2
-1
0
1
2 at 178.613 kb on + strand, within GFF5302 at 178.614 kb on - strand, within GFF5302 at 178.770 kb on + strand at 178.770 kb on + strand at 178.770 kb on + strand at 178.770 kb on + strand at 178.771 kb on - strand at 178.771 kb on - strand at 178.817 kb on + strand at 178.818 kb on - strand at 178.871 kb on + strand at 178.897 kb on + strand at 178.897 kb on + strand at 178.897 kb on + strand at 178.898 kb on - strand at 179.059 kb on + strand, within GFF5303 at 179.059 kb on + strand, within GFF5303 at 179.060 kb on - strand, within GFF5303 at 179.219 kb on - strand, within GFF5303 at 179.219 kb on - strand, within GFF5303 at 179.219 kb on - strand, within GFF5303 at 179.326 kb on + strand, within GFF5303 at 179.326 kb on + strand, within GFF5303 at 179.327 kb on - strand, within GFF5303 at 179.459 kb on + strand, within GFF5303 at 179.459 kb on + strand, within GFF5303 at 179.503 kb on + strand, within GFF5303 at 179.503 kb on + strand, within GFF5303 at 179.503 kb on + strand, within GFF5303 at 179.503 kb on + strand, within GFF5303 at 179.503 kb on + strand, within GFF5303 at 179.503 kb on + strand, within GFF5303 at 179.503 kb on + strand, within GFF5303 at 179.503 kb on + strand, within GFF5303 at 179.503 kb on + strand, within GFF5303 at 179.503 kb on + strand, within GFF5303 at 179.503 kb on + strand, within GFF5303 at 179.503 kb on + strand, within GFF5303 at 179.503 kb on + strand, within GFF5303 at 179.503 kb on + strand, within GFF5303 at 179.503 kb on + strand, within GFF5303 at 179.504 kb on - strand, within GFF5303 at 179.504 kb on - strand, within GFF5303 at 179.504 kb on - strand, within GFF5303 at 179.504 kb on - strand, within GFF5303 at 179.504 kb on - strand, within GFF5303 at 179.504 kb on - strand, within GFF5303 at 179.504 kb on - strand, within GFF5303 at 179.504 kb on - strand, within GFF5303 at 179.504 kb on - strand, within GFF5303 at 179.504 kb on - strand, within GFF5303 at 179.504 kb on - strand, within GFF5303 at 179.573 kb on - strand at 179.599 kb on + strand at 179.599 kb on + strand at 179.600 kb on - strand at 179.721 kb on - strand at 179.721 kb on - strand at 179.958 kb on - strand, within GFF5304 at 179.958 kb on - strand, within GFF5304 at 179.958 kb on - strand, within GFF5304 at 180.014 kb on + strand, within GFF5304 at 180.014 kb on + strand, within GFF5304 at 180.113 kb on + strand, within GFF5304 at 180.113 kb on + strand, within GFF5304 at 180.113 kb on + strand, within GFF5304 at 180.113 kb on + strand, within GFF5304 at 180.114 kb on - strand, within GFF5304 at 180.114 kb on - strand, within GFF5304 at 180.410 kb on + strand, within GFF5304 at 180.746 kb on + strand, within GFF5304 at 180.747 kb on - strand, within GFF5304 at 180.747 kb on - strand, within GFF5304 at 180.747 kb on - strand, within GFF5304 at 180.872 kb on + strand, within GFF5304 at 180.873 kb on - strand, within GFF5304
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 178,613 + GFF5302 0.87 +0.1 178,614 - GFF5302 0.87 -0.0 178,770 + +0.1 178,770 + -0.3 178,770 + +0.2 178,770 + +1.1 178,771 - +1.6 178,771 - +0.2 178,817 + +0.4 178,818 - +1.6 178,871 + +0.1 178,897 + -0.8 178,897 + -0.4 178,897 + -0.6 178,898 - -0.4 179,059 + GFF5303 0.20 +0.2 179,059 + GFF5303 0.20 -0.2 179,060 - GFF5303 0.20 -0.8 179,219 - GFF5303 0.43 +0.6 179,219 - GFF5303 0.43 +1.1 179,219 - GFF5303 0.43 -0.5 179,326 + GFF5303 0.59 +1.4 179,326 + GFF5303 0.59 -0.2 179,327 - GFF5303 0.59 +0.3 179,459 + GFF5303 0.79 +0.5 179,459 + GFF5303 0.79 -0.3 179,503 + GFF5303 0.85 -0.2 179,503 + GFF5303 0.85 +2.4 179,503 + GFF5303 0.85 -0.3 179,503 + GFF5303 0.85 +0.1 179,503 + GFF5303 0.85 -0.8 179,503 + GFF5303 0.85 -1.1 179,503 + GFF5303 0.85 -0.2 179,503 + GFF5303 0.85 +1.3 179,503 + GFF5303 0.85 -0.3 179,503 + GFF5303 0.85 -0.0 179,503 + GFF5303 0.85 -0.8 179,503 + GFF5303 0.85 +0.3 179,503 + GFF5303 0.85 -0.3 179,503 + GFF5303 0.85 +2.3 179,503 + GFF5303 0.85 -0.3 179,504 - GFF5303 0.85 +1.2 179,504 - GFF5303 0.85 -0.7 179,504 - GFF5303 0.85 +0.3 179,504 - GFF5303 0.85 +0.8 179,504 - GFF5303 0.85 -0.7 179,504 - GFF5303 0.85 +1.0 179,504 - GFF5303 0.85 -0.2 179,504 - GFF5303 0.85 -0.7 179,504 - GFF5303 0.85 -0.7 179,504 - GFF5303 0.85 +0.6 179,504 - GFF5303 0.85 +0.1 179,573 - -1.1 179,599 + +1.6 179,599 + +0.5 179,600 - -0.0 179,721 - -0.2 179,721 - -0.8 179,958 - GFF5304 0.25 -0.3 179,958 - GFF5304 0.25 +1.1 179,958 - GFF5304 0.25 +1.1 180,014 + GFF5304 0.29 -0.6 180,014 + GFF5304 0.29 -0.2 180,113 + GFF5304 0.35 -0.3 180,113 + GFF5304 0.35 +0.4 180,113 + GFF5304 0.35 -1.2 180,113 + GFF5304 0.35 +0.6 180,114 - GFF5304 0.36 -1.2 180,114 - GFF5304 0.36 +0.8 180,410 + GFF5304 0.56 +1.1 180,746 + GFF5304 0.80 -0.4 180,747 - GFF5304 0.80 -2.4 180,747 - GFF5304 0.80 -1.7 180,747 - GFF5304 0.80 +0.2 180,872 + GFF5304 0.88 +0.7 180,873 - GFF5304 0.88 +0.5
Or see this region's nucleotide sequence