Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5299 and GFF5300 are separated by 57 nucleotides GFF5300 and GFF5301 are separated by 17 nucleotides GFF5301 and GFF5302 are separated by 2 nucleotides
GFF5299 - TonB-dependent receptor, at 173,805 to 175,940
GFF5299
GFF5300 - ABC transporter, permease protein (cluster 10, nitrate/sulfonate/bicarbonate), at 175,998 to 176,873
GFF5300
GFF5301 - ABC transporter, ATP-binding protein (cluster 1, maltose/g3p/polyamine/iron); ABC transporter, ATP-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 176,891 to 177,709
GFF5301
GFF5302 - ABC transporter, substrate-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 177,712 to 178,752
GFF5302
Position (kb) 175
176
177 Strain fitness (log2 ratio) -2
-1
0
1 at 175.107 kb on + strand, within GFF5299 at 175.107 kb on + strand, within GFF5299 at 175.108 kb on - strand, within GFF5299 at 175.108 kb on - strand, within GFF5299 at 175.108 kb on - strand, within GFF5299 at 175.260 kb on + strand, within GFF5299 at 175.521 kb on + strand, within GFF5299 at 175.522 kb on - strand, within GFF5299 at 175.522 kb on - strand, within GFF5299 at 175.522 kb on - strand, within GFF5299 at 175.522 kb on - strand, within GFF5299 at 175.522 kb on - strand, within GFF5299 at 175.522 kb on - strand, within GFF5299 at 175.522 kb on - strand, within GFF5299 at 175.522 kb on - strand, within GFF5299 at 175.542 kb on + strand, within GFF5299 at 175.542 kb on + strand, within GFF5299 at 175.762 kb on - strand at 175.839 kb on + strand at 175.839 kb on + strand at 175.839 kb on + strand at 175.840 kb on - strand at 175.881 kb on + strand at 175.881 kb on + strand at 175.882 kb on - strand at 175.882 kb on - strand at 175.882 kb on - strand at 176.314 kb on + strand, within GFF5300 at 176.314 kb on + strand, within GFF5300 at 176.314 kb on + strand, within GFF5300 at 176.314 kb on + strand, within GFF5300 at 176.314 kb on + strand, within GFF5300 at 176.314 kb on + strand, within GFF5300 at 176.315 kb on - strand, within GFF5300 at 176.315 kb on - strand, within GFF5300 at 176.315 kb on - strand, within GFF5300 at 176.473 kb on + strand, within GFF5300 at 176.473 kb on + strand, within GFF5300 at 176.473 kb on + strand, within GFF5300 at 176.473 kb on + strand, within GFF5300 at 176.474 kb on - strand, within GFF5300 at 176.647 kb on + strand, within GFF5300 at 176.648 kb on - strand, within GFF5300 at 176.648 kb on - strand, within GFF5300 at 176.648 kb on - strand, within GFF5300 at 176.669 kb on - strand, within GFF5300 at 177.006 kb on + strand, within GFF5301 at 177.006 kb on + strand, within GFF5301 at 177.007 kb on - strand, within GFF5301 at 177.743 kb on + strand at 177.744 kb on - strand at 177.755 kb on + strand at 177.756 kb on - strand at 177.800 kb on + strand at 177.801 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 175,107 + GFF5299 0.61 -2.3 175,107 + GFF5299 0.61 +0.0 175,108 - GFF5299 0.61 +0.3 175,108 - GFF5299 0.61 +0.4 175,108 - GFF5299 0.61 +0.5 175,260 + GFF5299 0.68 -0.4 175,521 + GFF5299 0.80 +0.4 175,522 - GFF5299 0.80 -0.9 175,522 - GFF5299 0.80 +0.1 175,522 - GFF5299 0.80 -2.4 175,522 - GFF5299 0.80 +0.1 175,522 - GFF5299 0.80 -0.5 175,522 - GFF5299 0.80 +0.9 175,522 - GFF5299 0.80 +0.1 175,522 - GFF5299 0.80 +0.8 175,542 + GFF5299 0.81 -1.1 175,542 + GFF5299 0.81 +0.9 175,762 - -0.5 175,839 + -0.1 175,839 + -1.8 175,839 + +0.8 175,840 - -0.4 175,881 + -0.8 175,881 + -0.6 175,882 - -0.4 175,882 - -1.2 175,882 - -1.7 176,314 + GFF5300 0.36 +0.5 176,314 + GFF5300 0.36 -1.4 176,314 + GFF5300 0.36 -1.1 176,314 + GFF5300 0.36 +0.1 176,314 + GFF5300 0.36 +0.3 176,314 + GFF5300 0.36 +0.6 176,315 - GFF5300 0.36 +0.8 176,315 - GFF5300 0.36 +0.4 176,315 - GFF5300 0.36 +0.8 176,473 + GFF5300 0.54 -0.2 176,473 + GFF5300 0.54 +0.4 176,473 + GFF5300 0.54 -0.2 176,473 + GFF5300 0.54 -0.1 176,474 - GFF5300 0.54 -1.5 176,647 + GFF5300 0.74 +0.4 176,648 - GFF5300 0.74 -0.2 176,648 - GFF5300 0.74 -0.5 176,648 - GFF5300 0.74 +1.1 176,669 - GFF5300 0.77 +0.5 177,006 + GFF5301 0.14 -0.5 177,006 + GFF5301 0.14 -0.4 177,007 - GFF5301 0.14 +0.4 177,743 + -0.1 177,744 - -0.6 177,755 + -0.2 177,756 - +0.1 177,800 + -0.4 177,801 - -0.9
Or see this region's nucleotide sequence