Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5187 and GFF5188 are separated by 778 nucleotides GFF5188 and GFF5189 are separated by 85 nucleotides
GFF5187 - Aldose 1-epimerase (EC 5.1.3.3), at 44,205 to 45,284
GFF5187
GFF5188 - no description, at 46,063 to 47,025
GFF5188
GFF5189 - L-arabinolactonase (EC 3.1.1.15), at 47,111 to 48,064
GFF5189
Position (kb)
46
47
48 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 45.097 kb on - strand, within GFF5187 at 45.499 kb on + strand at 45.500 kb on - strand at 45.500 kb on - strand at 45.500 kb on - strand at 45.658 kb on + strand at 45.703 kb on + strand at 45.703 kb on + strand at 45.704 kb on - strand at 45.704 kb on - strand at 45.826 kb on + strand at 45.826 kb on + strand at 45.827 kb on - strand at 45.827 kb on - strand at 45.827 kb on - strand at 45.827 kb on - strand at 45.827 kb on - strand at 45.827 kb on - strand at 45.827 kb on - strand at 45.880 kb on + strand at 45.881 kb on - strand at 45.952 kb on + strand at 45.952 kb on + strand at 45.952 kb on + strand at 45.952 kb on + strand at 45.952 kb on + strand at 45.952 kb on + strand at 45.952 kb on + strand at 45.952 kb on + strand at 45.952 kb on + strand at 45.952 kb on + strand at 45.952 kb on + strand at 45.952 kb on + strand at 45.953 kb on - strand at 45.953 kb on - strand at 45.953 kb on - strand at 45.953 kb on - strand at 45.953 kb on - strand at 45.956 kb on + strand at 45.956 kb on + strand at 45.956 kb on + strand at 45.957 kb on - strand at 45.957 kb on - strand at 46.026 kb on + strand at 46.027 kb on - strand at 46.030 kb on + strand at 46.109 kb on + strand at 46.109 kb on + strand at 46.163 kb on + strand, within GFF5188 at 46.832 kb on + strand, within GFF5188 at 46.833 kb on - strand, within GFF5188 at 47.052 kb on + strand at 47.053 kb on - strand at 47.053 kb on - strand at 47.056 kb on + strand at 47.056 kb on + strand at 47.056 kb on + strand at 47.056 kb on + strand at 47.057 kb on - strand at 47.057 kb on - strand at 47.057 kb on - strand at 47.057 kb on - strand at 47.057 kb on - strand at 47.057 kb on - strand at 47.057 kb on - strand at 47.135 kb on + strand at 47.135 kb on + strand at 47.232 kb on + strand, within GFF5189 at 47.232 kb on + strand, within GFF5189 at 47.232 kb on + strand, within GFF5189 at 47.232 kb on + strand, within GFF5189 at 47.232 kb on + strand, within GFF5189 at 47.233 kb on - strand, within GFF5189 at 47.233 kb on - strand, within GFF5189 at 47.233 kb on - strand, within GFF5189 at 47.233 kb on - strand, within GFF5189 at 47.233 kb on - strand, within GFF5189 at 47.288 kb on + strand, within GFF5189 at 47.289 kb on - strand, within GFF5189 at 47.289 kb on - strand, within GFF5189 at 47.476 kb on - strand, within GFF5189 at 47.481 kb on + strand, within GFF5189 at 47.481 kb on + strand, within GFF5189 at 47.481 kb on + strand, within GFF5189 at 47.511 kb on + strand, within GFF5189 at 47.511 kb on + strand, within GFF5189 at 47.511 kb on + strand, within GFF5189 at 47.512 kb on - strand, within GFF5189 at 47.512 kb on - strand, within GFF5189 at 47.512 kb on - strand, within GFF5189 at 47.512 kb on - strand, within GFF5189 at 47.512 kb on - strand, within GFF5189 at 47.512 kb on - strand, within GFF5189 at 47.605 kb on - strand, within GFF5189 at 47.605 kb on - strand, within GFF5189 at 47.728 kb on - strand, within GFF5189 at 47.919 kb on + strand, within GFF5189 at 47.919 kb on + strand, within GFF5189 at 47.920 kb on - strand, within GFF5189 at 47.920 kb on - strand, within GFF5189 at 47.920 kb on - strand, within GFF5189 at 47.920 kb on - strand, within GFF5189 at 47.920 kb on - strand, within GFF5189
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 45,097 - GFF5187 0.83 -0.0 45,499 + -0.5 45,500 - -0.7 45,500 - +0.3 45,500 - +0.3 45,658 + +0.4 45,703 + -0.7 45,703 + -0.4 45,704 - +0.7 45,704 - +0.2 45,826 + +0.2 45,826 + +1.6 45,827 - -0.4 45,827 - -0.2 45,827 - +0.4 45,827 - +0.4 45,827 - -0.4 45,827 - +1.6 45,827 - -1.8 45,880 + -1.5 45,881 - -0.4 45,952 + +0.4 45,952 + -0.2 45,952 + -2.1 45,952 + -0.2 45,952 + +0.1 45,952 + -0.5 45,952 + +0.1 45,952 + +0.4 45,952 + -0.5 45,952 + -1.2 45,952 + -0.0 45,952 + +2.3 45,953 - -0.1 45,953 - +2.5 45,953 - +0.6 45,953 - -2.3 45,953 - +0.3 45,956 + -0.4 45,956 + +1.6 45,956 + -0.5 45,957 - +1.1 45,957 - -0.0 46,026 + +0.1 46,027 - -0.1 46,030 + -1.4 46,109 + -0.3 46,109 + +1.4 46,163 + GFF5188 0.10 -0.7 46,832 + GFF5188 0.80 -1.9 46,833 - GFF5188 0.80 -0.4 47,052 + +0.3 47,053 - -0.1 47,053 - +0.3 47,056 + -0.7 47,056 + -1.6 47,056 + +0.2 47,056 + -0.5 47,057 - +0.2 47,057 - +0.1 47,057 - -0.2 47,057 - +0.2 47,057 - -0.1 47,057 - +0.1 47,057 - -0.3 47,135 + -0.7 47,135 + -0.2 47,232 + GFF5189 0.13 -0.3 47,232 + GFF5189 0.13 -0.1 47,232 + GFF5189 0.13 +0.1 47,232 + GFF5189 0.13 -0.4 47,232 + GFF5189 0.13 -0.8 47,233 - GFF5189 0.13 -1.1 47,233 - GFF5189 0.13 -0.7 47,233 - GFF5189 0.13 -0.3 47,233 - GFF5189 0.13 -0.0 47,233 - GFF5189 0.13 -1.1 47,288 + GFF5189 0.19 -1.4 47,289 - GFF5189 0.19 +1.4 47,289 - GFF5189 0.19 -0.5 47,476 - GFF5189 0.38 +0.4 47,481 + GFF5189 0.39 +1.5 47,481 + GFF5189 0.39 -1.2 47,481 + GFF5189 0.39 +1.1 47,511 + GFF5189 0.42 +0.2 47,511 + GFF5189 0.42 -1.0 47,511 + GFF5189 0.42 +0.1 47,512 - GFF5189 0.42 +0.6 47,512 - GFF5189 0.42 -0.5 47,512 - GFF5189 0.42 +0.3 47,512 - GFF5189 0.42 +0.2 47,512 - GFF5189 0.42 -0.9 47,512 - GFF5189 0.42 +1.3 47,605 - GFF5189 0.52 -0.7 47,605 - GFF5189 0.52 -0.7 47,728 - GFF5189 0.65 -0.2 47,919 + GFF5189 0.85 -2.9 47,919 + GFF5189 0.85 +0.1 47,920 - GFF5189 0.85 -0.3 47,920 - GFF5189 0.85 +0.1 47,920 - GFF5189 0.85 -0.8 47,920 - GFF5189 0.85 -0.3 47,920 - GFF5189 0.85 -0.7
Or see this region's nucleotide sequence