Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4616 and GFF4617 overlap by 4 nucleotides GFF4617 and GFF4618 are separated by 110 nucleotides GFF4618 and GFF4619 are separated by 34 nucleotides
GFF4616 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 258,190 to 258,936
GFF4616
GFF4617 - 2-keto-3-deoxy-L-fuconate dehydrogenase, at 258,933 to 259,667
GFF4617
GFF4618 - Hexuronate utilization operon transcriptional repressor ExuR, at 259,778 to 260,485
GFF4618
GFF4619 - Altronate dehydratase (EC 4.2.1.7), at 260,520 to 262,058
GFF4619
Position (kb) 259
260
261 Strain fitness (log2 ratio) -2
-1
0
1
2 at 258.936 kb on + strand at 258.937 kb on - strand at 258.937 kb on - strand at 259.177 kb on + strand, within GFF4617 at 259.177 kb on + strand, within GFF4617 at 259.178 kb on - strand, within GFF4617 at 259.534 kb on + strand, within GFF4617 at 259.535 kb on - strand, within GFF4617 at 259.711 kb on - strand at 259.711 kb on - strand at 259.715 kb on + strand at 259.715 kb on + strand at 259.715 kb on - strand at 259.716 kb on - strand at 259.716 kb on - strand at 259.716 kb on - strand at 259.732 kb on - strand at 259.974 kb on + strand, within GFF4618 at 259.974 kb on + strand, within GFF4618 at 259.975 kb on - strand, within GFF4618 at 259.994 kb on + strand, within GFF4618 at 259.995 kb on - strand, within GFF4618 at 259.995 kb on - strand, within GFF4618 at 259.995 kb on - strand, within GFF4618 at 260.316 kb on - strand, within GFF4618 at 260.319 kb on - strand, within GFF4618 at 260.319 kb on - strand, within GFF4618 at 260.529 kb on + strand at 260.530 kb on - strand at 260.530 kb on - strand at 260.682 kb on + strand, within GFF4619 at 260.683 kb on - strand, within GFF4619 at 260.683 kb on - strand, within GFF4619 at 260.777 kb on + strand, within GFF4619 at 260.895 kb on + strand, within GFF4619 at 260.895 kb on + strand, within GFF4619 at 260.895 kb on + strand, within GFF4619 at 260.896 kb on - strand, within GFF4619 at 260.896 kb on - strand, within GFF4619 at 260.896 kb on - strand, within GFF4619 at 260.896 kb on - strand, within GFF4619 at 260.896 kb on - strand, within GFF4619 at 260.896 kb on - strand, within GFF4619 at 261.096 kb on + strand, within GFF4619 at 261.096 kb on + strand, within GFF4619 at 261.462 kb on + strand, within GFF4619 at 261.462 kb on + strand, within GFF4619 at 261.463 kb on - strand, within GFF4619
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 258,936 + -0.5 258,937 - +0.5 258,937 - +1.2 259,177 + GFF4617 0.33 +0.7 259,177 + GFF4617 0.33 -1.4 259,178 - GFF4617 0.33 +0.6 259,534 + GFF4617 0.82 -0.6 259,535 - GFF4617 0.82 -0.6 259,711 - +0.2 259,711 - -0.7 259,715 + +0.1 259,715 + +0.1 259,715 - -0.5 259,716 - -1.2 259,716 - -0.5 259,716 - +1.2 259,732 - -0.0 259,974 + GFF4618 0.28 -0.6 259,974 + GFF4618 0.28 -0.1 259,975 - GFF4618 0.28 +2.6 259,994 + GFF4618 0.31 -0.0 259,995 - GFF4618 0.31 +1.8 259,995 - GFF4618 0.31 +0.9 259,995 - GFF4618 0.31 +1.6 260,316 - GFF4618 0.76 +0.6 260,319 - GFF4618 0.76 +0.2 260,319 - GFF4618 0.76 -0.4 260,529 + +0.1 260,530 - +0.2 260,530 - -1.5 260,682 + GFF4619 0.11 -0.9 260,683 - GFF4619 0.11 -0.6 260,683 - GFF4619 0.11 -0.6 260,777 + GFF4619 0.17 +0.3 260,895 + GFF4619 0.24 -0.3 260,895 + GFF4619 0.24 -0.1 260,895 + GFF4619 0.24 +0.5 260,896 - GFF4619 0.24 +0.3 260,896 - GFF4619 0.24 -0.9 260,896 - GFF4619 0.24 -0.0 260,896 - GFF4619 0.24 +0.2 260,896 - GFF4619 0.24 -0.2 260,896 - GFF4619 0.24 -0.7 261,096 + GFF4619 0.37 -2.1 261,096 + GFF4619 0.37 -0.8 261,462 + GFF4619 0.61 -0.3 261,462 + GFF4619 0.61 +1.1 261,463 - GFF4619 0.61 -0.1
Or see this region's nucleotide sequence