Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4589 and GFF4590 are separated by 53 nucleotides GFF4590 and GFF4591 are separated by 27 nucleotides GFF4591 and GFF4592 are separated by 1 nucleotides GFF4592 and GFF4593 are separated by 98 nucleotides
GFF4589 - no description, at 230,063 to 231,118
GFF4589
GFF4590 - Glutamate/aspartate ABC transporter, ATP-binding protein GltL (TC 3.A.1.3.4), at 231,172 to 231,921
GFF4590
GFF4591 - Glutamate/aspartate ABC transporter, permease protein GltK (TC 3.A.1.3.4), at 231,949 to 232,617
GFF4591
GFF4592 - Glutamate/aspartate ABC transporter, permease protein GltJ (TC 3.A.1.3.4), at 232,619 to 233,377
GFF4592
GFF4593 - Glutamate/aspartate ABC transporter, substrate-binding protein GltI (TC 3.A.1.3.4), at 233,476 to 234,372
GFF4593
Position (kb)
231
232
233 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 231.057 kb on + strand at 231.878 kb on + strand at 231.879 kb on - strand at 231.889 kb on + strand at 231.889 kb on + strand at 231.889 kb on + strand at 231.889 kb on + strand at 231.889 kb on + strand at 231.890 kb on - strand at 232.004 kb on + strand at 232.004 kb on + strand at 232.005 kb on - strand at 232.019 kb on + strand, within GFF4591 at 232.020 kb on - strand, within GFF4591 at 232.040 kb on + strand, within GFF4591 at 232.041 kb on - strand, within GFF4591 at 232.041 kb on - strand, within GFF4591 at 232.041 kb on - strand, within GFF4591 at 232.041 kb on - strand, within GFF4591 at 232.041 kb on - strand, within GFF4591 at 232.115 kb on + strand, within GFF4591 at 232.116 kb on - strand, within GFF4591 at 232.116 kb on - strand, within GFF4591 at 232.116 kb on - strand, within GFF4591 at 232.116 kb on - strand, within GFF4591 at 232.220 kb on + strand, within GFF4591 at 232.220 kb on + strand, within GFF4591 at 232.220 kb on + strand, within GFF4591 at 232.220 kb on + strand, within GFF4591 at 232.220 kb on + strand, within GFF4591 at 232.220 kb on + strand, within GFF4591 at 232.220 kb on + strand, within GFF4591 at 232.220 kb on + strand, within GFF4591 at 232.220 kb on + strand, within GFF4591 at 232.220 kb on + strand, within GFF4591 at 232.221 kb on - strand, within GFF4591 at 232.221 kb on - strand, within GFF4591 at 232.221 kb on - strand, within GFF4591 at 232.301 kb on + strand, within GFF4591 at 232.301 kb on + strand, within GFF4591 at 232.301 kb on + strand, within GFF4591 at 232.302 kb on - strand, within GFF4591 at 232.302 kb on - strand, within GFF4591 at 232.302 kb on - strand, within GFF4591 at 232.323 kb on - strand, within GFF4591 at 232.436 kb on + strand, within GFF4591 at 232.437 kb on - strand, within GFF4591 at 232.437 kb on - strand, within GFF4591 at 232.550 kb on + strand, within GFF4591 at 232.550 kb on + strand, within GFF4591 at 232.551 kb on - strand at 232.551 kb on - strand at 232.551 kb on - strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.568 kb on + strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.569 kb on - strand at 232.592 kb on + strand at 232.592 kb on + strand at 232.592 kb on + strand at 232.592 kb on + strand at 232.593 kb on - strand at 232.593 kb on - strand at 232.719 kb on + strand, within GFF4592 at 232.719 kb on + strand, within GFF4592 at 232.719 kb on + strand, within GFF4592 at 232.719 kb on + strand, within GFF4592 at 232.719 kb on + strand, within GFF4592 at 232.719 kb on + strand, within GFF4592 at 232.719 kb on + strand, within GFF4592 at 232.719 kb on + strand, within GFF4592 at 232.719 kb on + strand, within GFF4592 at 232.720 kb on - strand, within GFF4592 at 232.720 kb on - strand, within GFF4592 at 232.720 kb on - strand, within GFF4592 at 232.720 kb on - strand, within GFF4592 at 232.720 kb on - strand, within GFF4592 at 232.720 kb on - strand, within GFF4592 at 232.720 kb on - strand, within GFF4592 at 232.720 kb on - strand, within GFF4592 at 232.720 kb on - strand, within GFF4592 at 232.720 kb on - strand, within GFF4592 at 232.740 kb on + strand, within GFF4592 at 232.740 kb on + strand, within GFF4592 at 232.741 kb on - strand, within GFF4592 at 232.741 kb on - strand, within GFF4592 at 232.782 kb on + strand, within GFF4592 at 232.782 kb on + strand, within GFF4592 at 232.908 kb on + strand, within GFF4592 at 232.908 kb on + strand, within GFF4592 at 232.908 kb on + strand, within GFF4592 at 232.908 kb on + strand, within GFF4592 at 232.909 kb on - strand, within GFF4592 at 232.909 kb on - strand, within GFF4592 at 232.911 kb on + strand, within GFF4592 at 232.911 kb on + strand, within GFF4592 at 232.911 kb on + strand, within GFF4592 at 232.911 kb on + strand, within GFF4592 at 232.911 kb on + strand, within GFF4592 at 232.911 kb on + strand, within GFF4592 at 232.911 kb on + strand, within GFF4592 at 232.911 kb on + strand, within GFF4592 at 232.912 kb on - strand, within GFF4592 at 232.912 kb on - strand, within GFF4592 at 232.912 kb on - strand, within GFF4592 at 232.948 kb on - strand, within GFF4592 at 233.091 kb on + strand, within GFF4592 at 233.091 kb on + strand, within GFF4592 at 233.092 kb on - strand, within GFF4592 at 233.092 kb on - strand, within GFF4592 at 233.092 kb on - strand, within GFF4592 at 233.092 kb on - strand, within GFF4592 at 233.194 kb on + strand, within GFF4592 at 233.195 kb on - strand, within GFF4592 at 233.195 kb on - strand, within GFF4592 at 233.195 kb on - strand, within GFF4592 at 233.195 kb on - strand, within GFF4592 at 233.195 kb on - strand, within GFF4592 at 233.286 kb on + strand, within GFF4592 at 233.286 kb on + strand, within GFF4592 at 233.286 kb on + strand, within GFF4592 at 233.287 kb on - strand, within GFF4592 at 233.287 kb on - strand, within GFF4592 at 233.287 kb on - strand, within GFF4592 at 233.287 kb on - strand, within GFF4592 at 233.287 kb on - strand, within GFF4592 at 233.412 kb on + strand at 233.412 kb on + strand at 233.412 kb on + strand at 233.412 kb on + strand at 233.412 kb on + strand at 233.412 kb on + strand at 233.412 kb on + strand at 233.413 kb on - strand at 233.413 kb on - strand at 233.413 kb on - strand at 233.413 kb on - strand at 233.413 kb on - strand at 233.413 kb on - strand at 233.477 kb on + strand at 233.477 kb on + strand at 233.478 kb on - strand at 233.478 kb on - strand at 233.478 kb on - strand at 233.489 kb on + strand at 233.489 kb on + strand at 233.490 kb on - strand at 233.535 kb on + strand at 233.535 kb on + strand at 233.535 kb on + strand at 233.535 kb on + strand at 233.536 kb on - strand at 233.603 kb on + strand, within GFF4593 at 233.604 kb on - strand, within GFF4593
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 231,057 + +0.5 231,878 + -1.0 231,879 - -1.5 231,889 + -0.4 231,889 + -0.5 231,889 + -0.5 231,889 + -1.2 231,889 + -0.6 231,890 - -1.3 232,004 + -0.7 232,004 + -0.6 232,005 - +1.1 232,019 + GFF4591 0.10 -0.6 232,020 - GFF4591 0.11 +0.6 232,040 + GFF4591 0.14 -1.1 232,041 - GFF4591 0.14 +1.2 232,041 - GFF4591 0.14 -0.4 232,041 - GFF4591 0.14 +0.5 232,041 - GFF4591 0.14 +0.3 232,041 - GFF4591 0.14 -1.4 232,115 + GFF4591 0.25 -0.2 232,116 - GFF4591 0.25 +0.6 232,116 - GFF4591 0.25 -0.7 232,116 - GFF4591 0.25 -1.7 232,116 - GFF4591 0.25 +0.6 232,220 + GFF4591 0.41 -0.7 232,220 + GFF4591 0.41 -0.2 232,220 + GFF4591 0.41 -0.9 232,220 + GFF4591 0.41 +0.5 232,220 + GFF4591 0.41 +0.2 232,220 + GFF4591 0.41 -0.1 232,220 + GFF4591 0.41 -0.4 232,220 + GFF4591 0.41 -1.2 232,220 + GFF4591 0.41 -1.1 232,220 + GFF4591 0.41 -0.4 232,221 - GFF4591 0.41 -0.6 232,221 - GFF4591 0.41 -0.1 232,221 - GFF4591 0.41 -0.3 232,301 + GFF4591 0.53 -0.9 232,301 + GFF4591 0.53 -0.2 232,301 + GFF4591 0.53 -2.2 232,302 - GFF4591 0.53 -1.0 232,302 - GFF4591 0.53 -1.9 232,302 - GFF4591 0.53 -0.2 232,323 - GFF4591 0.56 -0.9 232,436 + GFF4591 0.73 -0.9 232,437 - GFF4591 0.73 -0.5 232,437 - GFF4591 0.73 -1.5 232,550 + GFF4591 0.90 +1.2 232,550 + GFF4591 0.90 -1.2 232,551 - -1.4 232,551 - -0.0 232,551 - +0.1 232,568 + -1.9 232,568 + -0.4 232,568 + +1.6 232,568 + -0.4 232,568 + -0.0 232,568 + -0.5 232,568 + +0.5 232,568 + -0.1 232,568 + -1.7 232,568 + -0.2 232,568 + +0.0 232,568 + -3.2 232,568 + -0.1 232,568 + -1.4 232,568 + +0.2 232,568 + -2.0 232,568 + +0.2 232,568 + -0.1 232,568 + -1.0 232,568 + +0.9 232,568 + +0.3 232,569 - +0.6 232,569 - -0.8 232,569 - +0.5 232,569 - +0.1 232,569 - -1.9 232,569 - -0.6 232,569 - +0.4 232,569 - +0.1 232,569 - -0.3 232,569 - +0.3 232,569 - -1.1 232,569 - -0.6 232,569 - +0.0 232,569 - +0.0 232,569 - +0.5 232,592 + -0.6 232,592 + -0.2 232,592 + -0.6 232,592 + +0.2 232,593 - +0.2 232,593 - -2.7 232,719 + GFF4592 0.13 -2.9 232,719 + GFF4592 0.13 -0.0 232,719 + GFF4592 0.13 +0.2 232,719 + GFF4592 0.13 -0.4 232,719 + GFF4592 0.13 -0.0 232,719 + GFF4592 0.13 -0.6 232,719 + GFF4592 0.13 -0.4 232,719 + GFF4592 0.13 -0.9 232,719 + GFF4592 0.13 -0.3 232,720 - GFF4592 0.13 +0.3 232,720 - GFF4592 0.13 -1.2 232,720 - GFF4592 0.13 -2.1 232,720 - GFF4592 0.13 -0.1 232,720 - GFF4592 0.13 -0.0 232,720 - GFF4592 0.13 -0.8 232,720 - GFF4592 0.13 +0.2 232,720 - GFF4592 0.13 -0.9 232,720 - GFF4592 0.13 -1.2 232,720 - GFF4592 0.13 +0.4 232,740 + GFF4592 0.16 -1.4 232,740 + GFF4592 0.16 +0.2 232,741 - GFF4592 0.16 -1.2 232,741 - GFF4592 0.16 +1.6 232,782 + GFF4592 0.21 -0.2 232,782 + GFF4592 0.21 -0.9 232,908 + GFF4592 0.38 +0.1 232,908 + GFF4592 0.38 +0.8 232,908 + GFF4592 0.38 -0.4 232,908 + GFF4592 0.38 -0.2 232,909 - GFF4592 0.38 +1.3 232,909 - GFF4592 0.38 +0.3 232,911 + GFF4592 0.38 -0.7 232,911 + GFF4592 0.38 -0.4 232,911 + GFF4592 0.38 +0.0 232,911 + GFF4592 0.38 -0.6 232,911 + GFF4592 0.38 -0.2 232,911 + GFF4592 0.38 +2.6 232,911 + GFF4592 0.38 +0.4 232,911 + GFF4592 0.38 -1.6 232,912 - GFF4592 0.39 +0.4 232,912 - GFF4592 0.39 +0.2 232,912 - GFF4592 0.39 +0.6 232,948 - GFF4592 0.43 -0.9 233,091 + GFF4592 0.62 -2.4 233,091 + GFF4592 0.62 -1.0 233,092 - GFF4592 0.62 +0.3 233,092 - GFF4592 0.62 -1.7 233,092 - GFF4592 0.62 -0.4 233,092 - GFF4592 0.62 +0.6 233,194 + GFF4592 0.76 -0.4 233,195 - GFF4592 0.76 -0.7 233,195 - GFF4592 0.76 +0.2 233,195 - GFF4592 0.76 -0.2 233,195 - GFF4592 0.76 +0.6 233,195 - GFF4592 0.76 -0.8 233,286 + GFF4592 0.88 -0.8 233,286 + GFF4592 0.88 -0.9 233,286 + GFF4592 0.88 -1.1 233,287 - GFF4592 0.88 -0.0 233,287 - GFF4592 0.88 -0.1 233,287 - GFF4592 0.88 -2.4 233,287 - GFF4592 0.88 -0.3 233,287 - GFF4592 0.88 -2.2 233,412 + +0.2 233,412 + -0.7 233,412 + +0.0 233,412 + -0.2 233,412 + -2.1 233,412 + +0.1 233,412 + -1.1 233,413 - +0.7 233,413 - +0.6 233,413 - +3.6 233,413 - -0.2 233,413 - -0.6 233,413 - -1.7 233,477 + +0.9 233,477 + -1.0 233,478 - -0.1 233,478 - -1.2 233,478 - -1.3 233,489 + +0.6 233,489 + -1.8 233,490 - +0.7 233,535 + -0.0 233,535 + -1.9 233,535 + -0.6 233,535 + -1.1 233,536 - -1.9 233,603 + GFF4593 0.14 -0.4 233,604 - GFF4593 0.14 +0.4
Or see this region's nucleotide sequence