Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4554 and GFF4555 are separated by 103 nucleotides GFF4555 and GFF4556 overlap by 4 nucleotides GFF4556 and GFF4557 overlap by 4 nucleotides
GFF4554 - Protease HtpX, at 191,095 to 191,970
GFF4554
GFF4555 - Putative transmembrane protein, at 192,074 to 192,829
GFF4555
GFF4556 - hypothetical protein, at 192,826 to 193,626
GFF4556
GFF4557 - Dihydroorotase (EC 3.5.2.3), at 193,623 to 194,654
GFF4557
Position (kb)
192
193 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 191.074 kb on - strand at 191.074 kb on - strand at 191.074 kb on - strand at 191.074 kb on - strand at 191.086 kb on + strand at 191.086 kb on + strand at 191.378 kb on + strand, within GFF4554 at 191.378 kb on + strand, within GFF4554 at 191.378 kb on + strand, within GFF4554 at 191.378 kb on + strand, within GFF4554 at 191.378 kb on + strand, within GFF4554 at 191.378 kb on + strand, within GFF4554 at 191.378 kb on + strand, within GFF4554 at 191.378 kb on + strand, within GFF4554 at 191.378 kb on + strand, within GFF4554 at 191.378 kb on + strand, within GFF4554 at 191.378 kb on + strand, within GFF4554 at 191.378 kb on + strand, within GFF4554 at 191.378 kb on + strand, within GFF4554 at 191.379 kb on - strand, within GFF4554 at 191.379 kb on - strand, within GFF4554 at 191.379 kb on - strand, within GFF4554 at 191.379 kb on - strand, within GFF4554 at 191.379 kb on - strand, within GFF4554 at 191.379 kb on - strand, within GFF4554 at 191.379 kb on - strand, within GFF4554 at 191.379 kb on - strand, within GFF4554 at 191.379 kb on - strand, within GFF4554 at 191.379 kb on - strand, within GFF4554 at 191.381 kb on + strand, within GFF4554 at 191.382 kb on - strand, within GFF4554 at 191.382 kb on - strand, within GFF4554 at 191.441 kb on + strand, within GFF4554 at 191.442 kb on - strand, within GFF4554 at 191.442 kb on - strand, within GFF4554 at 191.961 kb on + strand at 191.961 kb on + strand at 191.961 kb on + strand at 191.962 kb on - strand at 191.962 kb on - strand at 191.962 kb on - strand at 191.962 kb on - strand at 191.962 kb on - strand at 192.005 kb on + strand at 192.005 kb on + strand at 192.005 kb on + strand at 192.006 kb on - strand at 192.006 kb on - strand at 192.006 kb on - strand at 192.060 kb on + strand at 192.060 kb on + strand at 192.060 kb on + strand at 192.060 kb on + strand at 192.060 kb on + strand at 192.060 kb on + strand at 192.061 kb on - strand at 192.061 kb on - strand at 192.061 kb on - strand at 192.061 kb on - strand at 192.061 kb on - strand at 192.061 kb on - strand at 192.061 kb on - strand at 192.061 kb on - strand at 192.066 kb on + strand at 192.066 kb on + strand at 192.066 kb on + strand at 192.066 kb on + strand at 192.067 kb on - strand at 192.067 kb on - strand at 192.067 kb on - strand at 192.067 kb on - strand at 192.067 kb on - strand at 192.067 kb on - strand at 192.075 kb on + strand at 192.086 kb on + strand at 192.086 kb on + strand at 192.087 kb on - strand at 192.087 kb on - strand at 192.129 kb on + strand at 192.129 kb on + strand at 192.129 kb on + strand at 192.130 kb on - strand at 192.130 kb on - strand at 192.130 kb on - strand at 192.651 kb on + strand, within GFF4555 at 192.652 kb on - strand, within GFF4555 at 192.652 kb on - strand, within GFF4555 at 192.652 kb on - strand, within GFF4555 at 192.695 kb on + strand, within GFF4555 at 192.695 kb on + strand, within GFF4555 at 192.696 kb on - strand, within GFF4555 at 192.696 kb on - strand, within GFF4555 at 192.696 kb on - strand, within GFF4555 at 192.696 kb on - strand, within GFF4555 at 193.232 kb on + strand, within GFF4556 at 193.232 kb on + strand, within GFF4556 at 193.232 kb on + strand, within GFF4556 at 193.232 kb on + strand, within GFF4556 at 193.232 kb on + strand, within GFF4556 at 193.232 kb on + strand, within GFF4556 at 193.232 kb on + strand, within GFF4556 at 193.233 kb on - strand, within GFF4556 at 193.233 kb on - strand, within GFF4556 at 193.233 kb on - strand, within GFF4556 at 193.233 kb on - strand, within GFF4556 at 193.233 kb on - strand, within GFF4556 at 193.233 kb on - strand, within GFF4556 at 193.233 kb on - strand, within GFF4556 at 193.233 kb on - strand, within GFF4556 at 193.233 kb on - strand, within GFF4556 at 193.505 kb on + strand, within GFF4556 at 193.505 kb on + strand, within GFF4556 at 193.505 kb on + strand, within GFF4556 at 193.506 kb on - strand, within GFF4556 at 193.506 kb on - strand, within GFF4556 at 193.506 kb on - strand, within GFF4556 at 193.506 kb on - strand, within GFF4556 at 193.506 kb on - strand, within GFF4556 at 193.506 kb on - strand, within GFF4556 at 193.532 kb on + strand, within GFF4556 at 193.533 kb on - strand, within GFF4556 at 193.756 kb on + strand, within GFF4557 at 193.756 kb on + strand, within GFF4557 at 193.756 kb on + strand, within GFF4557 at 193.756 kb on + strand, within GFF4557 at 193.757 kb on - strand, within GFF4557 at 193.757 kb on - strand, within GFF4557
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 191,074 - +1.4 191,074 - +1.3 191,074 - +0.7 191,074 - -0.7 191,086 + -1.3 191,086 + -0.4 191,378 + GFF4554 0.32 +0.2 191,378 + GFF4554 0.32 -0.5 191,378 + GFF4554 0.32 +0.1 191,378 + GFF4554 0.32 +0.6 191,378 + GFF4554 0.32 -0.7 191,378 + GFF4554 0.32 -0.7 191,378 + GFF4554 0.32 -0.3 191,378 + GFF4554 0.32 +0.1 191,378 + GFF4554 0.32 -0.4 191,378 + GFF4554 0.32 +0.3 191,378 + GFF4554 0.32 +1.1 191,378 + GFF4554 0.32 +0.0 191,378 + GFF4554 0.32 +0.8 191,379 - GFF4554 0.32 -1.1 191,379 - GFF4554 0.32 +0.1 191,379 - GFF4554 0.32 +0.4 191,379 - GFF4554 0.32 -0.3 191,379 - GFF4554 0.32 +0.2 191,379 - GFF4554 0.32 +0.5 191,379 - GFF4554 0.32 -0.4 191,379 - GFF4554 0.32 -0.4 191,379 - GFF4554 0.32 +0.3 191,379 - GFF4554 0.32 -0.4 191,381 + GFF4554 0.33 -0.1 191,382 - GFF4554 0.33 -0.1 191,382 - GFF4554 0.33 -0.3 191,441 + GFF4554 0.39 -0.4 191,442 - GFF4554 0.40 -0.2 191,442 - GFF4554 0.40 -0.7 191,961 + -0.3 191,961 + -0.8 191,961 + -0.7 191,962 - -0.4 191,962 - -0.6 191,962 - -2.2 191,962 - -0.3 191,962 - +0.1 192,005 + +0.1 192,005 + -1.9 192,005 + +1.6 192,006 - -0.4 192,006 - +1.2 192,006 - -0.9 192,060 + -0.0 192,060 + +1.2 192,060 + -0.5 192,060 + -1.1 192,060 + +0.4 192,060 + +0.7 192,061 - -0.8 192,061 - -0.2 192,061 - +0.2 192,061 - -0.4 192,061 - -0.2 192,061 - +0.1 192,061 - +0.3 192,061 - -0.9 192,066 + +1.0 192,066 + +0.2 192,066 + +0.3 192,066 + +0.8 192,067 - +1.6 192,067 - -0.5 192,067 - +0.6 192,067 - -3.9 192,067 - -0.7 192,067 - +1.2 192,075 + -0.0 192,086 + +0.1 192,086 + -0.8 192,087 - +1.6 192,087 - +0.3 192,129 + -0.0 192,129 + +0.3 192,129 + -1.0 192,130 - +0.1 192,130 - -2.9 192,130 - -1.5 192,651 + GFF4555 0.76 -0.1 192,652 - GFF4555 0.76 -0.7 192,652 - GFF4555 0.76 -1.0 192,652 - GFF4555 0.76 -0.2 192,695 + GFF4555 0.82 -0.5 192,695 + GFF4555 0.82 -1.3 192,696 - GFF4555 0.82 -1.1 192,696 - GFF4555 0.82 +0.3 192,696 - GFF4555 0.82 +0.1 192,696 - GFF4555 0.82 +0.6 193,232 + GFF4556 0.51 -0.0 193,232 + GFF4556 0.51 -2.1 193,232 + GFF4556 0.51 -0.2 193,232 + GFF4556 0.51 -1.0 193,232 + GFF4556 0.51 +0.2 193,232 + GFF4556 0.51 +1.7 193,232 + GFF4556 0.51 +0.1 193,233 - GFF4556 0.51 -0.2 193,233 - GFF4556 0.51 -0.7 193,233 - GFF4556 0.51 -1.7 193,233 - GFF4556 0.51 -0.1 193,233 - GFF4556 0.51 -0.8 193,233 - GFF4556 0.51 -1.7 193,233 - GFF4556 0.51 -0.5 193,233 - GFF4556 0.51 -0.7 193,233 - GFF4556 0.51 +0.7 193,505 + GFF4556 0.85 +0.7 193,505 + GFF4556 0.85 -0.9 193,505 + GFF4556 0.85 -1.4 193,506 - GFF4556 0.85 +2.6 193,506 - GFF4556 0.85 +0.4 193,506 - GFF4556 0.85 -0.5 193,506 - GFF4556 0.85 +0.5 193,506 - GFF4556 0.85 +0.9 193,506 - GFF4556 0.85 +0.7 193,532 + GFF4556 0.88 +0.6 193,533 - GFF4556 0.88 -0.7 193,756 + GFF4557 0.13 -1.2 193,756 + GFF4557 0.13 +1.6 193,756 + GFF4557 0.13 -0.7 193,756 + GFF4557 0.13 +0.6 193,757 - GFF4557 0.13 +0.6 193,757 - GFF4557 0.13 -0.2
Or see this region's nucleotide sequence