Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4385 and GFF4386 overlap by 4 nucleotides GFF4386 and GFF4387 overlap by 4 nucleotides GFF4387 and GFF4388 overlap by 4 nucleotides
GFF4385 - ParA-like protein, at 14,638 to 15,276
GFF4385
GFF4386 - FIG00457034: hypothetical protein, at 15,273 to 15,557
GFF4386
GFF4387 - Glycosidases, at 15,554 to 16,099
GFF4387
GFF4388 - Type IV secretory pathway, protease TraF, at 16,096 to 16,695
GFF4388
Position (kb)
15
16
17 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 14.970 kb on + strand, within GFF4385 at 14.970 kb on + strand, within GFF4385 at 14.970 kb on + strand, within GFF4385 at 14.970 kb on + strand, within GFF4385 at 14.970 kb on + strand, within GFF4385 at 14.970 kb on + strand, within GFF4385 at 14.970 kb on + strand, within GFF4385 at 14.977 kb on + strand, within GFF4385 at 14.977 kb on + strand, within GFF4385 at 14.977 kb on + strand, within GFF4385 at 14.977 kb on + strand, within GFF4385 at 15.078 kb on + strand, within GFF4385 at 15.078 kb on + strand, within GFF4385 at 15.078 kb on + strand, within GFF4385 at 15.078 kb on + strand, within GFF4385 at 15.079 kb on - strand, within GFF4385 at 15.079 kb on - strand, within GFF4385 at 15.079 kb on - strand, within GFF4385 at 15.079 kb on - strand, within GFF4385 at 15.079 kb on - strand, within GFF4385 at 15.079 kb on - strand, within GFF4385 at 15.311 kb on + strand, within GFF4386 at 15.311 kb on + strand, within GFF4386 at 15.311 kb on + strand, within GFF4386 at 15.312 kb on - strand, within GFF4386 at 15.312 kb on - strand, within GFF4386 at 15.408 kb on + strand, within GFF4386 at 15.408 kb on + strand, within GFF4386 at 15.408 kb on + strand, within GFF4386 at 15.408 kb on + strand, within GFF4386 at 15.408 kb on + strand, within GFF4386 at 15.408 kb on + strand, within GFF4386 at 15.409 kb on - strand, within GFF4386 at 15.409 kb on - strand, within GFF4386 at 15.606 kb on + strand at 15.606 kb on + strand at 15.606 kb on + strand at 15.606 kb on + strand at 15.606 kb on + strand at 15.606 kb on + strand at 15.606 kb on + strand at 15.606 kb on + strand at 15.606 kb on + strand at 15.606 kb on + strand at 15.606 kb on + strand at 15.606 kb on + strand at 15.606 kb on + strand at 15.606 kb on + strand at 15.607 kb on - strand at 15.607 kb on - strand at 15.607 kb on - strand at 15.607 kb on - strand at 15.607 kb on - strand at 15.607 kb on - strand at 15.607 kb on - strand at 15.607 kb on - strand at 15.607 kb on - strand at 15.619 kb on - strand, within GFF4387 at 15.692 kb on + strand, within GFF4387 at 15.692 kb on + strand, within GFF4387 at 15.692 kb on + strand, within GFF4387 at 15.692 kb on + strand, within GFF4387 at 15.692 kb on + strand, within GFF4387 at 15.693 kb on - strand, within GFF4387 at 15.693 kb on - strand, within GFF4387 at 15.693 kb on - strand, within GFF4387 at 15.693 kb on - strand, within GFF4387 at 15.693 kb on - strand, within GFF4387 at 15.693 kb on - strand, within GFF4387 at 15.693 kb on - strand, within GFF4387 at 15.804 kb on - strand, within GFF4387 at 15.998 kb on + strand, within GFF4387 at 15.998 kb on + strand, within GFF4387 at 15.999 kb on - strand, within GFF4387 at 16.049 kb on + strand at 16.049 kb on + strand at 16.049 kb on + strand at 16.049 kb on + strand at 16.049 kb on + strand at 16.049 kb on + strand at 16.049 kb on + strand at 16.049 kb on + strand at 16.050 kb on - strand at 16.050 kb on - strand at 16.050 kb on - strand at 16.050 kb on - strand at 16.250 kb on - strand, within GFF4388 at 16.250 kb on - strand, within GFF4388 at 16.250 kb on - strand, within GFF4388 at 16.250 kb on - strand, within GFF4388 at 16.250 kb on - strand, within GFF4388 at 16.250 kb on - strand, within GFF4388 at 16.250 kb on - strand, within GFF4388 at 16.250 kb on - strand, within GFF4388 at 16.250 kb on - strand, within GFF4388 at 16.250 kb on - strand, within GFF4388 at 16.250 kb on - strand, within GFF4388 at 16.250 kb on - strand, within GFF4388 at 16.250 kb on - strand, within GFF4388 at 16.250 kb on - strand, within GFF4388 at 16.400 kb on - strand, within GFF4388 at 16.400 kb on - strand, within GFF4388 at 16.400 kb on - strand, within GFF4388 at 16.400 kb on - strand, within GFF4388 at 16.400 kb on - strand, within GFF4388 at 16.478 kb on + strand, within GFF4388 at 16.478 kb on + strand, within GFF4388 at 16.478 kb on + strand, within GFF4388 at 16.478 kb on + strand, within GFF4388 at 16.478 kb on + strand, within GFF4388 at 16.478 kb on + strand, within GFF4388 at 16.479 kb on - strand, within GFF4388 at 16.479 kb on - strand, within GFF4388 at 16.479 kb on - strand, within GFF4388 at 16.479 kb on - strand, within GFF4388 at 16.479 kb on - strand, within GFF4388 at 16.516 kb on - strand, within GFF4388 at 16.726 kb on + strand at 16.726 kb on + strand at 16.726 kb on + strand at 16.726 kb on + strand at 16.726 kb on + strand at 16.726 kb on + strand at 16.727 kb on - strand at 16.727 kb on - strand at 16.727 kb on - strand at 16.727 kb on - strand at 16.727 kb on - strand at 16.727 kb on - strand at 16.859 kb on + strand at 16.859 kb on + strand at 16.859 kb on + strand at 16.859 kb on + strand at 16.859 kb on + strand at 16.859 kb on + strand at 16.860 kb on - strand at 16.860 kb on - strand at 16.860 kb on - strand at 17.049 kb on + strand at 17.049 kb on + strand at 17.050 kb on - strand at 17.050 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 14,970 + GFF4385 0.52 +1.2 14,970 + GFF4385 0.52 +0.6 14,970 + GFF4385 0.52 +1.1 14,970 + GFF4385 0.52 -0.1 14,970 + GFF4385 0.52 -0.4 14,970 + GFF4385 0.52 +0.3 14,970 + GFF4385 0.52 +0.1 14,977 + GFF4385 0.53 +0.5 14,977 + GFF4385 0.53 -1.2 14,977 + GFF4385 0.53 -0.8 14,977 + GFF4385 0.53 -0.4 15,078 + GFF4385 0.69 -0.7 15,078 + GFF4385 0.69 +0.2 15,078 + GFF4385 0.69 +0.8 15,078 + GFF4385 0.69 -1.9 15,079 - GFF4385 0.69 -0.8 15,079 - GFF4385 0.69 -0.0 15,079 - GFF4385 0.69 -0.4 15,079 - GFF4385 0.69 -0.7 15,079 - GFF4385 0.69 +0.0 15,079 - GFF4385 0.69 -0.0 15,311 + GFF4386 0.13 +0.3 15,311 + GFF4386 0.13 -0.1 15,311 + GFF4386 0.13 -0.2 15,312 - GFF4386 0.14 -0.9 15,312 - GFF4386 0.14 -1.4 15,408 + GFF4386 0.47 -0.6 15,408 + GFF4386 0.47 -0.2 15,408 + GFF4386 0.47 -0.8 15,408 + GFF4386 0.47 +0.6 15,408 + GFF4386 0.47 -0.2 15,408 + GFF4386 0.47 -0.9 15,409 - GFF4386 0.48 -0.6 15,409 - GFF4386 0.48 -0.2 15,606 + -0.2 15,606 + +0.7 15,606 + +0.8 15,606 + -1.2 15,606 + +1.5 15,606 + +0.1 15,606 + -0.5 15,606 + -0.1 15,606 + +0.7 15,606 + +1.1 15,606 + +0.3 15,606 + +0.2 15,606 + -0.5 15,606 + -0.3 15,607 - -0.5 15,607 - -0.3 15,607 - -0.7 15,607 - +1.1 15,607 - +1.2 15,607 - -0.2 15,607 - -0.4 15,607 - -0.7 15,607 - +0.1 15,619 - GFF4387 0.12 -0.9 15,692 + GFF4387 0.25 -0.3 15,692 + GFF4387 0.25 -1.3 15,692 + GFF4387 0.25 -0.3 15,692 + GFF4387 0.25 -1.4 15,692 + GFF4387 0.25 +0.5 15,693 - GFF4387 0.25 -0.0 15,693 - GFF4387 0.25 +1.0 15,693 - GFF4387 0.25 -0.8 15,693 - GFF4387 0.25 -2.4 15,693 - GFF4387 0.25 -1.2 15,693 - GFF4387 0.25 +0.1 15,693 - GFF4387 0.25 -0.7 15,804 - GFF4387 0.46 +2.1 15,998 + GFF4387 0.81 +0.6 15,998 + GFF4387 0.81 -0.4 15,999 - GFF4387 0.82 -0.5 16,049 + +0.1 16,049 + -0.4 16,049 + -1.2 16,049 + -0.5 16,049 + -0.0 16,049 + -1.2 16,049 + -0.9 16,049 + +0.7 16,050 - -0.0 16,050 - -1.2 16,050 - -0.7 16,050 - +0.2 16,250 - GFF4388 0.26 -1.2 16,250 - GFF4388 0.26 -0.4 16,250 - GFF4388 0.26 +0.1 16,250 - GFF4388 0.26 -0.3 16,250 - GFF4388 0.26 +0.5 16,250 - GFF4388 0.26 -0.4 16,250 - GFF4388 0.26 +0.1 16,250 - GFF4388 0.26 -0.7 16,250 - GFF4388 0.26 +2.6 16,250 - GFF4388 0.26 -0.5 16,250 - GFF4388 0.26 -0.4 16,250 - GFF4388 0.26 -0.2 16,250 - GFF4388 0.26 -0.4 16,250 - GFF4388 0.26 +0.6 16,400 - GFF4388 0.51 -0.3 16,400 - GFF4388 0.51 +1.6 16,400 - GFF4388 0.51 +0.4 16,400 - GFF4388 0.51 +0.9 16,400 - GFF4388 0.51 -0.4 16,478 + GFF4388 0.64 +1.6 16,478 + GFF4388 0.64 +0.4 16,478 + GFF4388 0.64 +0.5 16,478 + GFF4388 0.64 +0.8 16,478 + GFF4388 0.64 -0.4 16,478 + GFF4388 0.64 +0.1 16,479 - GFF4388 0.64 -0.9 16,479 - GFF4388 0.64 -1.4 16,479 - GFF4388 0.64 -0.5 16,479 - GFF4388 0.64 -0.6 16,479 - GFF4388 0.64 +0.2 16,516 - GFF4388 0.70 -0.1 16,726 + -1.5 16,726 + -0.1 16,726 + -1.1 16,726 + +0.1 16,726 + +0.1 16,726 + -0.1 16,727 - -0.9 16,727 - -0.4 16,727 - -1.9 16,727 - +0.2 16,727 - +0.6 16,727 - -0.3 16,859 + +1.1 16,859 + +1.1 16,859 + -1.2 16,859 + -0.9 16,859 + +0.1 16,859 + -0.5 16,860 - +1.4 16,860 - +0.1 16,860 - -0.7 17,049 + +1.1 17,049 + +0.0 17,050 - -0.3 17,050 - +0.4
Or see this region's nucleotide sequence