Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4230 and GFF4231 are separated by 36 nucleotides GFF4231 and GFF4232 are separated by 76 nucleotides GFF4232 and GFF4233 overlap by 4 nucleotides
GFF4230 - Transcriptional regulator of pyridoxine metabolism / Pyridoxamine phosphate aminotransferase (EC 2.6.1.54), at 141,708 to 143,018
GFF4230
GFF4231 - no description, at 143,055 to 144,026
GFF4231
GFF4232 - no description, at 144,103 to 144,657
GFF4232
GFF4233 - no description, at 144,654 to 145,064
GFF4233
Position (kb)
143
144
145 Strain fitness (log2 ratio)
-2
-1
0
1 at 142.059 kb on + strand, within GFF4230 at 142.060 kb on - strand, within GFF4230 at 142.060 kb on - strand, within GFF4230 at 142.135 kb on - strand, within GFF4230 at 142.441 kb on - strand, within GFF4230 at 142.497 kb on + strand, within GFF4230 at 142.498 kb on - strand, within GFF4230 at 142.540 kb on + strand, within GFF4230 at 142.540 kb on + strand, within GFF4230 at 142.541 kb on - strand, within GFF4230 at 142.541 kb on - strand, within GFF4230 at 142.541 kb on - strand, within GFF4230 at 142.735 kb on - strand, within GFF4230 at 143.356 kb on + strand, within GFF4231 at 143.356 kb on + strand, within GFF4231 at 143.356 kb on + strand, within GFF4231 at 143.356 kb on + strand, within GFF4231 at 143.356 kb on + strand, within GFF4231 at 143.357 kb on - strand, within GFF4231 at 143.357 kb on - strand, within GFF4231 at 143.527 kb on + strand, within GFF4231 at 143.528 kb on - strand, within GFF4231 at 143.774 kb on - strand, within GFF4231 at 143.774 kb on - strand, within GFF4231 at 144.033 kb on - strand at 144.304 kb on + strand, within GFF4232 at 144.304 kb on + strand, within GFF4232 at 144.305 kb on - strand, within GFF4232 at 144.511 kb on + strand, within GFF4232 at 144.511 kb on + strand, within GFF4232 at 144.512 kb on - strand, within GFF4232 at 144.512 kb on - strand, within GFF4232 at 144.720 kb on + strand, within GFF4233 at 144.721 kb on - strand, within GFF4233 at 144.786 kb on + strand, within GFF4233 at 144.817 kb on - strand, within GFF4233
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 142,059 + GFF4230 0.27 +0.2 142,060 - GFF4230 0.27 +0.2 142,060 - GFF4230 0.27 -0.0 142,135 - GFF4230 0.33 +0.2 142,441 - GFF4230 0.56 +0.2 142,497 + GFF4230 0.60 +0.0 142,498 - GFF4230 0.60 -0.2 142,540 + GFF4230 0.63 +0.4 142,540 + GFF4230 0.63 +0.6 142,541 - GFF4230 0.64 +0.4 142,541 - GFF4230 0.64 -0.3 142,541 - GFF4230 0.64 -0.0 142,735 - GFF4230 0.78 -0.1 143,356 + GFF4231 0.31 -1.8 143,356 + GFF4231 0.31 -0.5 143,356 + GFF4231 0.31 +0.0 143,356 + GFF4231 0.31 -2.5 143,356 + GFF4231 0.31 +0.6 143,357 - GFF4231 0.31 +0.4 143,357 - GFF4231 0.31 -1.7 143,527 + GFF4231 0.49 -1.4 143,528 - GFF4231 0.49 -0.4 143,774 - GFF4231 0.74 -0.5 143,774 - GFF4231 0.74 +0.6 144,033 - -0.0 144,304 + GFF4232 0.36 -0.4 144,304 + GFF4232 0.36 +0.5 144,305 - GFF4232 0.36 -0.4 144,511 + GFF4232 0.74 -1.7 144,511 + GFF4232 0.74 +1.0 144,512 - GFF4232 0.74 -0.7 144,512 - GFF4232 0.74 -0.6 144,720 + GFF4233 0.16 +0.4 144,721 - GFF4233 0.16 -1.4 144,786 + GFF4233 0.32 -0.7 144,817 - GFF4233 0.40 -0.4
Or see this region's nucleotide sequence