Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4214 and GFF4215 are separated by 2 nucleotides GFF4215 and GFF4216 are separated by 87 nucleotides GFF4216 and GFF4217 are separated by 159 nucleotides
GFF4214 - L-amino acid ABC transporter (Glu/Asp/His/...), permease protein 2 AapM, at 129,064 to 130,173
GFF4214
GFF4215 - L-amino acid ABC transporter (Glu/Asp/His/...), permease protein 1 AapQ, at 130,176 to 130,856
GFF4215
GFF4216 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 130,944 to 131,288
GFF4216
GFF4217 - Glutamate/aspartate ABC transporter, substrate-binding protein GltI (TC 3.A.1.3.4), at 131,448 to 132,464
GFF4217
Position (kb) 130
131
132 Strain fitness (log2 ratio) -2
-1
0
1 at 130.208 kb on - strand at 130.379 kb on - strand, within GFF4215 at 130.543 kb on + strand, within GFF4215 at 130.543 kb on + strand, within GFF4215 at 130.972 kb on + strand at 131.020 kb on - strand, within GFF4216 at 131.282 kb on - strand at 131.390 kb on + strand at 131.391 kb on - strand at 131.391 kb on - strand at 131.467 kb on + strand at 131.467 kb on + strand at 131.467 kb on + strand at 131.468 kb on - strand at 131.468 kb on - strand at 131.488 kb on + strand at 131.489 kb on - strand at 131.568 kb on + strand, within GFF4217 at 131.587 kb on + strand, within GFF4217 at 131.588 kb on - strand, within GFF4217 at 131.588 kb on - strand, within GFF4217 at 131.588 kb on - strand, within GFF4217 at 131.707 kb on + strand, within GFF4217 at 131.707 kb on + strand, within GFF4217
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 130,208 - -0.4 130,379 - GFF4215 0.30 +0.3 130,543 + GFF4215 0.54 -0.4 130,543 + GFF4215 0.54 -0.5 130,972 + +0.1 131,020 - GFF4216 0.22 -1.3 131,282 - +0.2 131,390 + -2.4 131,391 - -0.3 131,391 - +0.7 131,467 + -0.5 131,467 + -0.7 131,467 + +1.6 131,468 - +0.5 131,468 - -0.1 131,488 + -0.0 131,489 - -0.2 131,568 + GFF4217 0.12 -0.3 131,587 + GFF4217 0.14 -0.4 131,588 - GFF4217 0.14 +0.2 131,588 - GFF4217 0.14 -0.0 131,588 - GFF4217 0.14 -0.1 131,707 + GFF4217 0.25 -0.2 131,707 + GFF4217 0.25 +0.4
Or see this region's nucleotide sequence