Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4165 and GFF4166 overlap by 11 nucleotides GFF4166 and GFF4167 overlap by 4 nucleotides GFF4167 and GFF4168 are separated by 1 nucleotides GFF4168 and GFF4169 overlap by 4 nucleotides
GFF4165 - Deoxyribose-phosphate aldolase (EC 4.1.2.4), at 70,247 to 71,041
GFF4165
GFF4166 - Cytidine deaminase (EC 3.5.4.5), at 71,031 to 71,501
GFF4166
GFF4167 - ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines) / ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines), at 71,498 to 73,120
GFF4167
GFF4168 - ABC transporter, permease protein 2 (cluster 5, nickel/peptides/opines), at 73,122 to 73,937
GFF4168
GFF4169 - ABC transporter, permease protein 1 (cluster 5, nickel/peptides/opines), at 73,934 to 74,878
GFF4169
Position (kb) 71
72
73
74 Strain fitness (log2 ratio) -2
-1
0
1
2 at 71.419 kb on + strand, within GFF4166 at 71.566 kb on - strand at 72.314 kb on + strand, within GFF4167 at 72.314 kb on + strand, within GFF4167 at 72.366 kb on + strand, within GFF4167 at 72.366 kb on + strand, within GFF4167 at 72.367 kb on - strand, within GFF4167 at 72.367 kb on - strand, within GFF4167 at 72.367 kb on - strand, within GFF4167 at 73.453 kb on + strand, within GFF4168 at 73.454 kb on - strand, within GFF4168 at 73.454 kb on - strand, within GFF4168 at 73.454 kb on - strand, within GFF4168 at 73.454 kb on - strand, within GFF4168 at 73.968 kb on + strand at 74.001 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 71,419 + GFF4166 0.82 -0.5 71,566 - +0.5 72,314 + GFF4167 0.50 +0.5 72,314 + GFF4167 0.50 +0.8 72,366 + GFF4167 0.53 -0.9 72,366 + GFF4167 0.53 +0.5 72,367 - GFF4167 0.54 -1.0 72,367 - GFF4167 0.54 +1.5 72,367 - GFF4167 0.54 -1.8 73,453 + GFF4168 0.41 +2.0 73,454 - GFF4168 0.41 -1.0 73,454 - GFF4168 0.41 -0.4 73,454 - GFF4168 0.41 +0.3 73,454 - GFF4168 0.41 +0.6 73,968 + -0.1 74,001 + -0.5
Or see this region's nucleotide sequence