Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4152 and GFF4153 are separated by 28 nucleotides GFF4153 and GFF4154 overlap by 28 nucleotides GFF4154 and GFF4155 overlap by 4 nucleotides GFF4155 and GFF4156 overlap by 14 nucleotides
GFF4152 - ABC transporter, substrate-binding protein (cluster 4, leucine/isoleucine/valine/benzoate), at 58,408 to 59,574
GFF4152
GFF4153 - ABC transporter, permease protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 59,603 to 60,544
GFF4153
GFF4154 - ABC transporter, permease protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 60,517 to 61,371
GFF4154
GFF4155 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 61,368 to 62,138
GFF4155
GFF4156 - ABC transporter, ATP-binding protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 62,125 to 62,865
GFF4156
Position (kb) 60
61
62 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 59.654 kb on + strand at 59.654 kb on + strand at 59.705 kb on + strand, within GFF4153 at 59.850 kb on - strand, within GFF4153 at 59.960 kb on + strand, within GFF4153 at 59.960 kb on + strand, within GFF4153 at 60.014 kb on + strand, within GFF4153 at 60.269 kb on + strand, within GFF4153 at 60.269 kb on + strand, within GFF4153 at 60.270 kb on - strand, within GFF4153 at 60.526 kb on + strand at 60.649 kb on + strand, within GFF4154 at 60.893 kb on - strand, within GFF4154 at 61.090 kb on + strand, within GFF4154 at 61.091 kb on - strand, within GFF4154 at 61.096 kb on + strand, within GFF4154 at 61.096 kb on + strand, within GFF4154 at 61.097 kb on - strand, within GFF4154 at 61.097 kb on - strand, within GFF4154 at 61.097 kb on - strand, within GFF4154 at 61.099 kb on + strand, within GFF4154 at 61.099 kb on + strand, within GFF4154 at 61.100 kb on - strand, within GFF4154 at 61.255 kb on + strand, within GFF4154 at 62.104 kb on - strand at 62.104 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 59,654 + -1.4 59,654 + +0.0 59,705 + GFF4153 0.11 -0.8 59,850 - GFF4153 0.26 -0.3 59,960 + GFF4153 0.38 -0.7 59,960 + GFF4153 0.38 -0.2 60,014 + GFF4153 0.44 -0.3 60,269 + GFF4153 0.71 +0.4 60,269 + GFF4153 0.71 -1.8 60,270 - GFF4153 0.71 -0.2 60,526 + +1.6 60,649 + GFF4154 0.15 -3.4 60,893 - GFF4154 0.44 +0.3 61,090 + GFF4154 0.67 -1.2 61,091 - GFF4154 0.67 -0.5 61,096 + GFF4154 0.68 -0.2 61,096 + GFF4154 0.68 +0.3 61,097 - GFF4154 0.68 -1.0 61,097 - GFF4154 0.68 +0.3 61,097 - GFF4154 0.68 -0.7 61,099 + GFF4154 0.68 -0.5 61,099 + GFF4154 0.68 -0.3 61,100 - GFF4154 0.68 -0.9 61,255 + GFF4154 0.86 +0.6 62,104 - -0.2 62,104 - -1.5
Or see this region's nucleotide sequence