Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF409 and GFF410 are separated by 14 nucleotides GFF410 and GFF411 overlap by 4 nucleotides GFF411 and GFF412 are separated by 119 nucleotides
GFF409 - Osmolarity sensory histidine kinase EnvZ, at 443,143 to 444,564
GFF409
GFF410 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (EC 4.6.1.12), at 444,579 to 445,067
GFF410
GFF411 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (EC 2.7.7.60), at 445,064 to 445,786
GFF411
GFF412 - Transcription-repair coupling factor, at 445,906 to 449,388
GFF412
Position (kb) 444
445
446 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 443.596 kb on + strand, within GFF409 at 443.596 kb on + strand, within GFF409 at 443.596 kb on + strand, within GFF409 at 443.596 kb on + strand, within GFF409 at 443.596 kb on + strand, within GFF409 at 443.597 kb on - strand, within GFF409 at 443.597 kb on - strand, within GFF409 at 443.597 kb on - strand, within GFF409 at 443.597 kb on - strand, within GFF409 at 443.597 kb on - strand, within GFF409 at 443.770 kb on + strand, within GFF409 at 443.771 kb on - strand, within GFF409 at 443.771 kb on - strand, within GFF409 at 443.771 kb on - strand, within GFF409 at 443.944 kb on + strand, within GFF409 at 443.945 kb on - strand, within GFF409 at 444.097 kb on + strand, within GFF409 at 444.097 kb on + strand, within GFF409 at 444.112 kb on + strand, within GFF409 at 444.112 kb on + strand, within GFF409 at 444.112 kb on + strand, within GFF409 at 444.113 kb on - strand, within GFF409 at 444.288 kb on - strand, within GFF409 at 444.491 kb on + strand at 444.491 kb on + strand at 444.491 kb on + strand at 444.491 kb on + strand at 444.492 kb on - strand at 444.492 kb on - strand at 444.492 kb on - strand at 444.492 kb on - strand at 444.492 kb on - strand at 444.513 kb on + strand at 444.513 kb on + strand at 444.513 kb on + strand at 444.514 kb on - strand at 444.514 kb on - strand at 444.514 kb on - strand at 444.514 kb on - strand at 444.514 kb on - strand at 444.514 kb on - strand at 444.514 kb on - strand at 445.797 kb on - strand at 445.841 kb on + strand at 445.841 kb on + strand at 445.896 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 443,596 + GFF409 0.32 -1.9 443,596 + GFF409 0.32 +1.3 443,596 + GFF409 0.32 -1.9 443,596 + GFF409 0.32 -1.3 443,596 + GFF409 0.32 +0.3 443,597 - GFF409 0.32 -0.3 443,597 - GFF409 0.32 -3.0 443,597 - GFF409 0.32 -1.0 443,597 - GFF409 0.32 -0.3 443,597 - GFF409 0.32 -1.1 443,770 + GFF409 0.44 -2.8 443,771 - GFF409 0.44 -0.3 443,771 - GFF409 0.44 -3.2 443,771 - GFF409 0.44 -2.5 443,944 + GFF409 0.56 -1.5 443,945 - GFF409 0.56 -0.3 444,097 + GFF409 0.67 -0.7 444,097 + GFF409 0.67 +0.3 444,112 + GFF409 0.68 -2.5 444,112 + GFF409 0.68 -2.2 444,112 + GFF409 0.68 +0.3 444,113 - GFF409 0.68 -0.5 444,288 - GFF409 0.81 +0.9 444,491 + -0.3 444,491 + -0.7 444,491 + -0.2 444,491 + -0.7 444,492 - -0.3 444,492 - -0.7 444,492 - -0.3 444,492 - -1.6 444,492 - -0.3 444,513 + -1.3 444,513 + +1.3 444,513 + -0.3 444,514 - +2.6 444,514 - +1.9 444,514 - +0.2 444,514 - +0.3 444,514 - -0.2 444,514 - -0.4 444,514 - -0.7 445,797 - +0.8 445,841 + -0.2 445,841 + -0.8 445,896 + -0.3
Or see this region's nucleotide sequence