Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3982 and GFF3983 overlap by 4 nucleotides GFF3983 and GFF3984 overlap by 4 nucleotides GFF3984 and GFF3985 overlap by 11 nucleotides
GFF3982 - Branched-chain amino acid ABC transporter, permease protein LivH (TC 3.A.1.4.1), at 185,550 to 186,605
GFF3982
GFF3983 - ABC transporter, permease protein 2 (cluster 4, leucine/isoleucine/valine/benzoate) / ABC transporter, ATP-binding protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 186,602 to 188,389
GFF3983
GFF3984 - Branched-chain amino acid ABC transporter, ATP-binding protein LivF (TC 3.A.1.4.1), at 188,386 to 189,117
GFF3984
GFF3985 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 189,107 to 190,696
GFF3985
Position (kb) 186
187
188
189 Strain fitness (log2 ratio) -2
-1
0
1
2 at 185.608 kb on - strand at 186.300 kb on + strand, within GFF3982 at 186.363 kb on + strand, within GFF3982 at 186.363 kb on + strand, within GFF3982 at 186.363 kb on + strand, within GFF3982 at 186.363 kb on + strand, within GFF3982 at 186.363 kb on + strand, within GFF3982 at 186.363 kb on + strand, within GFF3982 at 186.364 kb on - strand, within GFF3982 at 186.364 kb on - strand, within GFF3982 at 186.367 kb on - strand, within GFF3982 at 187.076 kb on + strand, within GFF3983 at 187.077 kb on - strand, within GFF3983 at 187.077 kb on - strand, within GFF3983 at 187.077 kb on - strand, within GFF3983 at 187.079 kb on + strand, within GFF3983 at 187.080 kb on - strand, within GFF3983 at 187.271 kb on + strand, within GFF3983 at 187.271 kb on + strand, within GFF3983 at 187.272 kb on - strand, within GFF3983 at 187.331 kb on + strand, within GFF3983 at 188.385 kb on + strand at 188.386 kb on - strand at 188.746 kb on + strand, within GFF3984 at 189.007 kb on + strand, within GFF3984 at 189.008 kb on - strand, within GFF3984 at 189.008 kb on - strand, within GFF3984 at 189.085 kb on + strand at 189.086 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 185,608 - -0.0 186,300 + GFF3982 0.71 +0.1 186,363 + GFF3982 0.77 -0.5 186,363 + GFF3982 0.77 -0.2 186,363 + GFF3982 0.77 -0.8 186,363 + GFF3982 0.77 +1.2 186,363 + GFF3982 0.77 -1.2 186,363 + GFF3982 0.77 -0.0 186,364 - GFF3982 0.77 -0.1 186,364 - GFF3982 0.77 +1.0 186,367 - GFF3982 0.77 +1.8 187,076 + GFF3983 0.27 +0.3 187,077 - GFF3983 0.27 +0.2 187,077 - GFF3983 0.27 +0.3 187,077 - GFF3983 0.27 -0.8 187,079 + GFF3983 0.27 +0.3 187,080 - GFF3983 0.27 -2.2 187,271 + GFF3983 0.37 +0.4 187,271 + GFF3983 0.37 +1.0 187,272 - GFF3983 0.37 +0.8 187,331 + GFF3983 0.41 +1.0 188,385 + -0.7 188,386 - -0.5 188,746 + GFF3984 0.49 +0.5 189,007 + GFF3984 0.85 +0.1 189,008 - GFF3984 0.85 +0.6 189,008 - GFF3984 0.85 +0.7 189,085 + +0.2 189,086 - -0.3
Or see this region's nucleotide sequence