Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3967 and GFF3968 are separated by 4 nucleotides GFF3968 and GFF3969 are separated by 13 nucleotides
GFF3967 - Indolepyruvate oxidoreductase subunit IorA (EC 1.2.7.8), at 167,972 to 170,164
GFF3967
GFF3968 - Indolepyruvate oxidoreductase subunit IorB II (EC 1.2.7.8), at 170,169 to 171,674
GFF3968
GFF3969 - Flavodoxin reductases (ferredoxin-NADPH reductases) family 1; Vanillate O-demethylase oxidoreductase (EC 1.14.13.-), at 171,688 to 172,680
GFF3969
Position (kb)
170
171
172 Strain fitness (log2 ratio)
-2
-1
0
1 at 169.553 kb on + strand, within GFF3967 at 169.553 kb on + strand, within GFF3967 at 169.553 kb on + strand, within GFF3967 at 169.554 kb on - strand, within GFF3967 at 169.554 kb on - strand, within GFF3967 at 169.554 kb on - strand, within GFF3967 at 169.686 kb on - strand, within GFF3967 at 169.686 kb on - strand, within GFF3967 at 169.686 kb on - strand, within GFF3967 at 170.042 kb on + strand at 170.043 kb on - strand at 170.154 kb on - strand at 170.331 kb on + strand, within GFF3968 at 170.331 kb on + strand, within GFF3968 at 170.331 kb on + strand, within GFF3968 at 170.332 kb on - strand, within GFF3968 at 170.332 kb on - strand, within GFF3968 at 170.332 kb on - strand, within GFF3968 at 170.973 kb on + strand, within GFF3968 at 170.985 kb on + strand, within GFF3968 at 171.186 kb on + strand, within GFF3968 at 171.186 kb on + strand, within GFF3968 at 171.186 kb on + strand, within GFF3968 at 171.187 kb on - strand, within GFF3968 at 171.187 kb on - strand, within GFF3968 at 171.331 kb on - strand, within GFF3968 at 171.387 kb on + strand, within GFF3968 at 171.387 kb on + strand, within GFF3968 at 171.388 kb on - strand, within GFF3968 at 171.388 kb on - strand, within GFF3968 at 171.502 kb on - strand, within GFF3968 at 171.534 kb on + strand at 171.534 kb on + strand at 171.534 kb on + strand at 171.534 kb on + strand at 171.919 kb on + strand, within GFF3969 at 171.919 kb on + strand, within GFF3969 at 172.294 kb on + strand, within GFF3969 at 172.634 kb on + strand at 172.634 kb on + strand at 172.635 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 169,553 + GFF3967 0.72 +0.7 169,553 + GFF3967 0.72 -0.9 169,553 + GFF3967 0.72 -0.9 169,554 - GFF3967 0.72 -0.0 169,554 - GFF3967 0.72 -0.0 169,554 - GFF3967 0.72 +0.5 169,686 - GFF3967 0.78 -0.3 169,686 - GFF3967 0.78 -0.4 169,686 - GFF3967 0.78 -0.3 170,042 + -0.7 170,043 - -0.0 170,154 - -0.5 170,331 + GFF3968 0.11 -0.6 170,331 + GFF3968 0.11 -0.2 170,331 + GFF3968 0.11 -0.6 170,332 - GFF3968 0.11 -0.4 170,332 - GFF3968 0.11 -0.7 170,332 - GFF3968 0.11 -1.2 170,973 + GFF3968 0.53 -0.3 170,985 + GFF3968 0.54 -0.6 171,186 + GFF3968 0.68 +0.7 171,186 + GFF3968 0.68 -0.3 171,186 + GFF3968 0.68 -0.6 171,187 - GFF3968 0.68 +0.7 171,187 - GFF3968 0.68 -0.5 171,331 - GFF3968 0.77 -0.5 171,387 + GFF3968 0.81 -0.8 171,387 + GFF3968 0.81 +0.2 171,388 - GFF3968 0.81 -2.0 171,388 - GFF3968 0.81 +1.0 171,502 - GFF3968 0.89 -1.1 171,534 + -0.7 171,534 + +0.6 171,534 + -0.1 171,534 + +0.2 171,919 + GFF3969 0.23 +0.2 171,919 + GFF3969 0.23 -1.0 172,294 + GFF3969 0.61 -1.0 172,634 + -0.0 172,634 + -0.8 172,635 - +0.0
Or see this region's nucleotide sequence