Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3963 and GFF3964 overlap by 4 nucleotides GFF3964 and GFF3965 overlap by 4 nucleotides GFF3965 and GFF3966 are separated by 41 nucleotides
GFF3962 - Opine dehydrogenase (EC 1.5.1.28), at 163,561 to 164,634
GFF3962
GFF3963 - 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35), at 164,624 to 165,628
GFF3963
GFF3964 - 2-hydroxymuconic semialdehyde hydrolase (EC 3.7.1.9), at 165,625 to 166,428
GFF3964
GFF3965 - Alcohol dehydrogenase, zinc-binding, at 166,425 to 167,309
GFF3965
GFF3966 - Homoprotocatechuate degradative operon repressor, at 167,351 to 167,827
GFF3966
Position (kb) 165
166
167 Strain fitness (log2 ratio) -1
0
1
2 at 164.705 kb on + strand at 164.793 kb on + strand, within GFF3963 at 165.102 kb on - strand, within GFF3963 at 165.102 kb on - strand, within GFF3963 at 165.440 kb on + strand, within GFF3963 at 165.512 kb on + strand, within GFF3963 at 165.512 kb on + strand, within GFF3963 at 165.513 kb on - strand, within GFF3963 at 165.519 kb on - strand, within GFF3963 at 165.676 kb on + strand at 165.676 kb on + strand at 165.749 kb on - strand, within GFF3964 at 165.749 kb on - strand, within GFF3964 at 165.912 kb on - strand, within GFF3964 at 165.912 kb on - strand, within GFF3964 at 165.912 kb on - strand, within GFF3964 at 166.003 kb on + strand, within GFF3964 at 166.003 kb on + strand, within GFF3964 at 166.003 kb on + strand, within GFF3964 at 166.004 kb on - strand, within GFF3964 at 166.594 kb on - strand, within GFF3965 at 166.745 kb on - strand, within GFF3965 at 166.982 kb on - strand, within GFF3965 at 166.982 kb on - strand, within GFF3965 at 166.982 kb on - strand, within GFF3965 at 166.985 kb on + strand, within GFF3965 at 166.985 kb on + strand, within GFF3965 at 166.985 kb on + strand, within GFF3965 at 166.985 kb on + strand, within GFF3965 at 166.985 kb on + strand, within GFF3965 at 166.986 kb on - strand, within GFF3965 at 166.986 kb on - strand, within GFF3965 at 166.986 kb on - strand, within GFF3965 at 166.986 kb on - strand, within GFF3965 at 166.986 kb on - strand, within GFF3965 at 166.986 kb on - strand, within GFF3965 at 166.986 kb on - strand, within GFF3965 at 167.058 kb on + strand, within GFF3965
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 164,705 + +1.8 164,793 + GFF3963 0.17 +1.1 165,102 - GFF3963 0.48 -0.4 165,102 - GFF3963 0.48 -0.1 165,440 + GFF3963 0.81 +1.0 165,512 + GFF3963 0.88 +0.2 165,512 + GFF3963 0.88 -0.7 165,513 - GFF3963 0.88 +0.9 165,519 - GFF3963 0.89 -0.0 165,676 + -0.3 165,676 + +0.5 165,749 - GFF3964 0.15 +0.4 165,749 - GFF3964 0.15 -0.5 165,912 - GFF3964 0.36 -0.6 165,912 - GFF3964 0.36 -0.5 165,912 - GFF3964 0.36 +0.5 166,003 + GFF3964 0.47 -0.4 166,003 + GFF3964 0.47 -1.0 166,003 + GFF3964 0.47 +0.2 166,004 - GFF3964 0.47 -0.5 166,594 - GFF3965 0.19 +0.2 166,745 - GFF3965 0.36 +1.1 166,982 - GFF3965 0.63 +0.0 166,982 - GFF3965 0.63 -0.6 166,982 - GFF3965 0.63 -0.3 166,985 + GFF3965 0.63 -0.5 166,985 + GFF3965 0.63 -0.8 166,985 + GFF3965 0.63 -0.1 166,985 + GFF3965 0.63 +0.2 166,985 + GFF3965 0.63 -0.7 166,986 - GFF3965 0.63 +1.0 166,986 - GFF3965 0.63 -0.8 166,986 - GFF3965 0.63 -0.0 166,986 - GFF3965 0.63 -0.3 166,986 - GFF3965 0.63 -0.1 166,986 - GFF3965 0.63 +0.2 166,986 - GFF3965 0.63 -0.4 167,058 + GFF3965 0.72 -0.5
Or see this region's nucleotide sequence