Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3834 and GFF3835 overlap by 4 nucleotides GFF3835 and GFF3836 are separated by 64 nucleotides GFF3836 and GFF3837 overlap by 4 nucleotides GFF3837 and GFF3838 are separated by 166 nucleotides
GFF3834 - hypothetical protein, at 25,011 to 25,556
GFF3834
GFF3835 - ADP-ribosylglycohydrolase family protein, at 25,553 to 25,954
GFF3835
GFF3836 - Cyclic beta-1,2-glucan synthase (EC 2.4.1.-), at 26,019 to 34,184
GFF3836
GFF3837 - no description, at 34,181 to 34,399
GFF3837
GFF3838 - Phosphoenolpyruvate carboxylase (EC 4.1.1.31), at 34,566 to 37,409
GFF3838
Position (kb) 26
27
28
29
30
31
32
33
34
35 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 25.019 kb on - strand at 25.019 kb on - strand at 25.019 kb on - strand at 25.019 kb on - strand at 25.380 kb on - strand, within GFF3834 at 25.468 kb on + strand, within GFF3834 at 25.688 kb on + strand, within GFF3835 at 25.688 kb on + strand, within GFF3835 at 25.689 kb on - strand, within GFF3835 at 25.689 kb on - strand, within GFF3835 at 25.689 kb on - strand, within GFF3835 at 26.001 kb on - strand at 26.281 kb on + strand at 26.281 kb on + strand at 26.308 kb on + strand at 26.308 kb on + strand at 26.308 kb on - strand at 26.309 kb on - strand at 26.309 kb on - strand at 26.309 kb on - strand at 26.323 kb on + strand at 26.323 kb on + strand at 26.323 kb on + strand at 26.324 kb on - strand at 26.324 kb on - strand at 26.345 kb on - strand at 26.485 kb on + strand at 26.486 kb on - strand at 26.486 kb on - strand at 26.530 kb on + strand at 26.531 kb on - strand at 27.067 kb on + strand, within GFF3836 at 27.067 kb on + strand, within GFF3836 at 27.067 kb on + strand, within GFF3836 at 27.067 kb on + strand, within GFF3836 at 27.068 kb on - strand, within GFF3836 at 27.068 kb on - strand, within GFF3836 at 27.068 kb on - strand, within GFF3836 at 27.070 kb on + strand, within GFF3836 at 27.070 kb on + strand, within GFF3836 at 27.071 kb on - strand, within GFF3836 at 27.071 kb on - strand, within GFF3836 at 27.071 kb on - strand, within GFF3836 at 27.160 kb on + strand, within GFF3836 at 27.176 kb on - strand, within GFF3836 at 27.421 kb on + strand, within GFF3836 at 27.809 kb on + strand, within GFF3836 at 28.053 kb on - strand, within GFF3836 at 28.053 kb on - strand, within GFF3836 at 28.054 kb on + strand, within GFF3836 at 28.054 kb on + strand, within GFF3836 at 28.054 kb on + strand, within GFF3836 at 28.054 kb on + strand, within GFF3836 at 28.054 kb on + strand, within GFF3836 at 28.055 kb on - strand, within GFF3836 at 28.055 kb on - strand, within GFF3836 at 28.055 kb on - strand, within GFF3836 at 28.055 kb on - strand, within GFF3836 at 28.055 kb on - strand, within GFF3836 at 28.153 kb on + strand, within GFF3836 at 28.153 kb on + strand, within GFF3836 at 28.153 kb on + strand, within GFF3836 at 28.154 kb on - strand, within GFF3836 at 28.154 kb on - strand, within GFF3836 at 28.154 kb on - strand, within GFF3836 at 28.154 kb on - strand, within GFF3836 at 28.154 kb on - strand, within GFF3836 at 28.307 kb on + strand, within GFF3836 at 28.308 kb on - strand, within GFF3836 at 28.585 kb on + strand, within GFF3836 at 28.585 kb on + strand, within GFF3836 at 28.585 kb on + strand, within GFF3836 at 28.586 kb on - strand, within GFF3836 at 28.586 kb on - strand, within GFF3836 at 28.586 kb on - strand, within GFF3836 at 28.681 kb on + strand, within GFF3836 at 28.681 kb on + strand, within GFF3836 at 28.682 kb on - strand, within GFF3836 at 28.895 kb on - strand, within GFF3836 at 28.895 kb on - strand, within GFF3836 at 29.023 kb on + strand, within GFF3836 at 29.023 kb on + strand, within GFF3836 at 29.024 kb on - strand, within GFF3836 at 29.024 kb on - strand, within GFF3836 at 29.024 kb on - strand, within GFF3836 at 29.024 kb on - strand, within GFF3836 at 29.440 kb on + strand, within GFF3836 at 29.440 kb on + strand, within GFF3836 at 29.441 kb on - strand, within GFF3836 at 29.541 kb on + strand, within GFF3836 at 29.542 kb on - strand, within GFF3836 at 29.542 kb on - strand, within GFF3836 at 29.674 kb on + strand, within GFF3836 at 29.674 kb on + strand, within GFF3836 at 29.675 kb on - strand, within GFF3836 at 29.675 kb on - strand, within GFF3836 at 29.675 kb on - strand, within GFF3836 at 29.689 kb on + strand, within GFF3836 at 29.690 kb on - strand, within GFF3836 at 29.782 kb on + strand, within GFF3836 at 30.214 kb on + strand, within GFF3836 at 30.283 kb on + strand, within GFF3836 at 30.289 kb on + strand, within GFF3836 at 30.317 kb on - strand, within GFF3836 at 30.448 kb on + strand, within GFF3836 at 30.448 kb on + strand, within GFF3836 at 30.448 kb on + strand, within GFF3836 at 30.449 kb on - strand, within GFF3836 at 30.449 kb on - strand, within GFF3836 at 30.601 kb on + strand, within GFF3836 at 30.601 kb on + strand, within GFF3836 at 30.602 kb on - strand, within GFF3836 at 30.604 kb on + strand, within GFF3836 at 30.604 kb on + strand, within GFF3836 at 30.604 kb on + strand, within GFF3836 at 30.605 kb on - strand, within GFF3836 at 30.605 kb on - strand, within GFF3836 at 30.605 kb on - strand, within GFF3836 at 30.605 kb on - strand, within GFF3836 at 30.605 kb on - strand, within GFF3836 at 30.605 kb on - strand, within GFF3836 at 30.670 kb on + strand, within GFF3836 at 30.670 kb on + strand, within GFF3836 at 30.670 kb on + strand, within GFF3836 at 30.671 kb on - strand, within GFF3836 at 30.838 kb on + strand, within GFF3836 at 30.838 kb on + strand, within GFF3836 at 30.838 kb on + strand, within GFF3836 at 30.838 kb on + strand, within GFF3836 at 30.838 kb on + strand, within GFF3836 at 30.839 kb on - strand, within GFF3836 at 30.839 kb on - strand, within GFF3836 at 30.839 kb on - strand, within GFF3836 at 30.839 kb on - strand, within GFF3836 at 30.874 kb on + strand, within GFF3836 at 31.003 kb on + strand, within GFF3836 at 31.003 kb on + strand, within GFF3836 at 31.003 kb on + strand, within GFF3836 at 31.003 kb on + strand, within GFF3836 at 31.003 kb on + strand, within GFF3836 at 31.003 kb on + strand, within GFF3836 at 31.004 kb on - strand, within GFF3836 at 31.004 kb on - strand, within GFF3836 at 31.004 kb on - strand, within GFF3836 at 31.004 kb on - strand, within GFF3836 at 31.004 kb on - strand, within GFF3836 at 31.004 kb on - strand, within GFF3836 at 31.004 kb on - strand, within GFF3836 at 31.004 kb on - strand, within GFF3836 at 31.147 kb on + strand, within GFF3836 at 31.148 kb on - strand, within GFF3836 at 31.148 kb on - strand, within GFF3836 at 32.021 kb on - strand, within GFF3836 at 32.021 kb on - strand, within GFF3836 at 32.021 kb on - strand, within GFF3836 at 32.021 kb on - strand, within GFF3836 at 32.044 kb on + strand, within GFF3836 at 32.269 kb on + strand, within GFF3836 at 32.269 kb on + strand, within GFF3836 at 32.269 kb on + strand, within GFF3836 at 32.270 kb on - strand, within GFF3836 at 32.270 kb on - strand, within GFF3836 at 32.470 kb on + strand, within GFF3836 at 32.470 kb on + strand, within GFF3836 at 32.471 kb on - strand, within GFF3836 at 32.471 kb on - strand, within GFF3836 at 32.471 kb on - strand, within GFF3836 at 32.818 kb on - strand, within GFF3836 at 32.902 kb on + strand, within GFF3836 at 32.902 kb on + strand, within GFF3836 at 32.902 kb on + strand, within GFF3836 at 32.902 kb on + strand, within GFF3836 at 32.903 kb on - strand, within GFF3836 at 32.903 kb on - strand, within GFF3836 at 32.903 kb on - strand, within GFF3836 at 33.028 kb on + strand, within GFF3836 at 33.028 kb on + strand, within GFF3836 at 33.028 kb on + strand, within GFF3836 at 33.029 kb on - strand, within GFF3836 at 33.029 kb on - strand, within GFF3836 at 33.029 kb on - strand, within GFF3836 at 33.029 kb on - strand, within GFF3836 at 33.031 kb on + strand, within GFF3836 at 33.031 kb on + strand, within GFF3836 at 33.244 kb on - strand, within GFF3836 at 33.454 kb on + strand at 33.454 kb on + strand at 33.454 kb on + strand at 33.454 kb on + strand at 33.454 kb on + strand at 33.454 kb on + strand at 33.454 kb on + strand at 33.455 kb on - strand at 33.455 kb on - strand at 33.455 kb on - strand at 33.455 kb on - strand at 33.490 kb on + strand at 33.491 kb on - strand at 33.612 kb on + strand at 33.612 kb on + strand at 33.613 kb on - strand at 33.676 kb on + strand at 33.736 kb on + strand at 33.736 kb on + strand at 33.736 kb on + strand at 33.737 kb on + strand at 33.737 kb on - strand at 33.737 kb on - strand at 33.737 kb on - strand at 33.737 kb on - strand at 33.754 kb on + strand at 33.755 kb on - strand at 33.755 kb on - strand at 33.755 kb on - strand at 33.755 kb on - strand at 33.809 kb on - strand at 33.811 kb on + strand at 33.811 kb on + strand at 33.811 kb on + strand at 33.811 kb on + strand at 33.811 kb on + strand at 33.812 kb on - strand at 33.812 kb on - strand at 33.812 kb on - strand at 33.812 kb on - strand at 33.901 kb on + strand at 33.902 kb on - strand at 33.902 kb on - strand at 33.902 kb on - strand at 33.902 kb on - strand at 33.902 kb on - strand at 33.932 kb on - strand at 34.378 kb on + strand at 34.416 kb on + strand at 34.476 kb on + strand at 34.477 kb on - strand at 34.477 kb on - strand at 34.486 kb on + strand at 34.487 kb on - strand at 34.515 kb on + strand at 34.516 kb on - strand at 34.516 kb on - strand at 34.516 kb on - strand at 34.699 kb on + strand at 34.699 kb on + strand at 34.699 kb on + strand at 34.700 kb on - strand at 34.700 kb on - strand at 34.862 kb on - strand, within GFF3838 at 34.904 kb on - strand, within GFF3838 at 34.945 kb on + strand, within GFF3838 at 34.945 kb on + strand, within GFF3838 at 34.945 kb on + strand, within GFF3838 at 34.945 kb on + strand, within GFF3838 at 34.946 kb on - strand, within GFF3838 at 34.946 kb on - strand, within GFF3838 at 34.946 kb on - strand, within GFF3838
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 25,019 - +0.1 25,019 - +0.6 25,019 - +2.0 25,019 - -0.5 25,380 - GFF3834 0.68 -0.4 25,468 + GFF3834 0.84 -0.4 25,688 + GFF3835 0.34 -0.9 25,688 + GFF3835 0.34 +0.2 25,689 - GFF3835 0.34 +1.5 25,689 - GFF3835 0.34 +2.8 25,689 - GFF3835 0.34 +1.2 26,001 - -0.6 26,281 + -0.3 26,281 + -0.3 26,308 + -0.3 26,308 + -0.5 26,308 - +0.3 26,309 - -0.9 26,309 - +0.0 26,309 - +0.2 26,323 + -0.7 26,323 + -0.2 26,323 + -1.3 26,324 - +0.4 26,324 - -0.8 26,345 - -0.1 26,485 + +0.7 26,486 - -0.1 26,486 - +0.7 26,530 + -0.1 26,531 - -0.0 27,067 + GFF3836 0.13 +0.3 27,067 + GFF3836 0.13 -0.5 27,067 + GFF3836 0.13 -0.3 27,067 + GFF3836 0.13 +1.5 27,068 - GFF3836 0.13 +0.3 27,068 - GFF3836 0.13 -0.6 27,068 - GFF3836 0.13 +0.2 27,070 + GFF3836 0.13 -0.3 27,070 + GFF3836 0.13 +0.6 27,071 - GFF3836 0.13 -0.4 27,071 - GFF3836 0.13 +0.7 27,071 - GFF3836 0.13 +0.5 27,160 + GFF3836 0.14 +0.5 27,176 - GFF3836 0.14 -0.2 27,421 + GFF3836 0.17 +0.0 27,809 + GFF3836 0.22 -0.5 28,053 - GFF3836 0.25 -0.6 28,053 - GFF3836 0.25 +1.2 28,054 + GFF3836 0.25 -0.1 28,054 + GFF3836 0.25 -0.3 28,054 + GFF3836 0.25 -0.0 28,054 + GFF3836 0.25 -0.4 28,054 + GFF3836 0.25 -1.0 28,055 - GFF3836 0.25 +1.7 28,055 - GFF3836 0.25 -1.3 28,055 - GFF3836 0.25 +1.0 28,055 - GFF3836 0.25 -0.1 28,055 - GFF3836 0.25 -1.2 28,153 + GFF3836 0.26 +1.2 28,153 + GFF3836 0.26 -0.9 28,153 + GFF3836 0.26 -0.3 28,154 - GFF3836 0.26 -0.2 28,154 - GFF3836 0.26 -1.0 28,154 - GFF3836 0.26 +0.0 28,154 - GFF3836 0.26 -0.1 28,154 - GFF3836 0.26 -0.5 28,307 + GFF3836 0.28 +1.1 28,308 - GFF3836 0.28 -0.4 28,585 + GFF3836 0.31 +0.1 28,585 + GFF3836 0.31 -0.1 28,585 + GFF3836 0.31 +0.1 28,586 - GFF3836 0.31 -1.9 28,586 - GFF3836 0.31 -0.0 28,586 - GFF3836 0.31 -0.1 28,681 + GFF3836 0.33 +0.4 28,681 + GFF3836 0.33 +0.4 28,682 - GFF3836 0.33 -0.3 28,895 - GFF3836 0.35 +0.5 28,895 - GFF3836 0.35 +0.2 29,023 + GFF3836 0.37 +0.5 29,023 + GFF3836 0.37 -0.0 29,024 - GFF3836 0.37 +0.5 29,024 - GFF3836 0.37 +0.4 29,024 - GFF3836 0.37 +0.1 29,024 - GFF3836 0.37 -1.5 29,440 + GFF3836 0.42 +0.4 29,440 + GFF3836 0.42 -1.3 29,441 - GFF3836 0.42 +0.4 29,541 + GFF3836 0.43 +0.3 29,542 - GFF3836 0.43 -1.0 29,542 - GFF3836 0.43 +0.2 29,674 + GFF3836 0.45 -1.5 29,674 + GFF3836 0.45 +0.3 29,675 - GFF3836 0.45 -1.6 29,675 - GFF3836 0.45 +0.5 29,675 - GFF3836 0.45 +0.5 29,689 + GFF3836 0.45 -0.6 29,690 - GFF3836 0.45 +0.7 29,782 + GFF3836 0.46 -0.0 30,214 + GFF3836 0.51 -2.2 30,283 + GFF3836 0.52 -0.7 30,289 + GFF3836 0.52 -0.4 30,317 - GFF3836 0.53 -0.2 30,448 + GFF3836 0.54 -0.4 30,448 + GFF3836 0.54 -0.0 30,448 + GFF3836 0.54 +0.3 30,449 - GFF3836 0.54 -0.5 30,449 - GFF3836 0.54 -0.8 30,601 + GFF3836 0.56 -1.9 30,601 + GFF3836 0.56 -0.5 30,602 - GFF3836 0.56 +0.0 30,604 + GFF3836 0.56 -0.2 30,604 + GFF3836 0.56 +0.2 30,604 + GFF3836 0.56 -0.3 30,605 - GFF3836 0.56 -0.2 30,605 - GFF3836 0.56 +0.8 30,605 - GFF3836 0.56 +0.1 30,605 - GFF3836 0.56 -2.2 30,605 - GFF3836 0.56 -0.6 30,605 - GFF3836 0.56 -2.6 30,670 + GFF3836 0.57 -0.8 30,670 + GFF3836 0.57 +2.0 30,670 + GFF3836 0.57 -0.8 30,671 - GFF3836 0.57 +2.0 30,838 + GFF3836 0.59 -1.3 30,838 + GFF3836 0.59 -0.3 30,838 + GFF3836 0.59 +0.1 30,838 + GFF3836 0.59 +1.0 30,838 + GFF3836 0.59 -0.8 30,839 - GFF3836 0.59 +0.1 30,839 - GFF3836 0.59 +0.0 30,839 - GFF3836 0.59 -0.1 30,839 - GFF3836 0.59 -0.1 30,874 + GFF3836 0.59 -0.1 31,003 + GFF3836 0.61 +0.4 31,003 + GFF3836 0.61 -1.0 31,003 + GFF3836 0.61 -0.1 31,003 + GFF3836 0.61 -0.3 31,003 + GFF3836 0.61 -0.7 31,003 + GFF3836 0.61 -0.4 31,004 - GFF3836 0.61 +0.8 31,004 - GFF3836 0.61 +0.3 31,004 - GFF3836 0.61 -0.0 31,004 - GFF3836 0.61 -1.0 31,004 - GFF3836 0.61 -0.5 31,004 - GFF3836 0.61 -1.0 31,004 - GFF3836 0.61 -0.7 31,004 - GFF3836 0.61 +1.0 31,147 + GFF3836 0.63 +0.4 31,148 - GFF3836 0.63 +0.2 31,148 - GFF3836 0.63 -0.3 32,021 - GFF3836 0.73 +2.0 32,021 - GFF3836 0.73 -0.0 32,021 - GFF3836 0.73 +0.1 32,021 - GFF3836 0.73 -1.3 32,044 + GFF3836 0.74 -0.1 32,269 + GFF3836 0.77 +0.6 32,269 + GFF3836 0.77 +0.3 32,269 + GFF3836 0.77 +0.7 32,270 - GFF3836 0.77 +1.5 32,270 - GFF3836 0.77 -0.4 32,470 + GFF3836 0.79 -1.9 32,470 + GFF3836 0.79 +0.2 32,471 - GFF3836 0.79 -0.4 32,471 - GFF3836 0.79 -0.0 32,471 - GFF3836 0.79 -0.4 32,818 - GFF3836 0.83 -1.5 32,902 + GFF3836 0.84 -0.5 32,902 + GFF3836 0.84 +0.6 32,902 + GFF3836 0.84 +0.7 32,902 + GFF3836 0.84 +0.5 32,903 - GFF3836 0.84 -1.6 32,903 - GFF3836 0.84 -0.3 32,903 - GFF3836 0.84 -1.0 33,028 + GFF3836 0.86 +0.1 33,028 + GFF3836 0.86 +0.8 33,028 + GFF3836 0.86 -0.6 33,029 - GFF3836 0.86 +1.0 33,029 - GFF3836 0.86 -1.3 33,029 - GFF3836 0.86 -0.2 33,029 - GFF3836 0.86 -1.0 33,031 + GFF3836 0.86 +1.5 33,031 + GFF3836 0.86 +0.5 33,244 - GFF3836 0.88 +1.5 33,454 + +1.4 33,454 + +1.3 33,454 + -0.3 33,454 + -2.0 33,454 + +0.5 33,454 + -1.8 33,454 + -0.4 33,455 - -0.3 33,455 - -0.5 33,455 - -1.8 33,455 - -1.2 33,490 + -0.0 33,491 - -1.2 33,612 + -0.8 33,612 + -0.0 33,613 - +0.7 33,676 + -0.1 33,736 + -0.7 33,736 + +0.3 33,736 + -0.5 33,737 + -0.6 33,737 - +0.3 33,737 - -0.1 33,737 - -0.2 33,737 - -0.3 33,754 + -1.5 33,755 - -0.8 33,755 - -0.0 33,755 - +0.1 33,755 - -0.9 33,809 - +1.2 33,811 + -1.9 33,811 + -1.0 33,811 + +0.2 33,811 + -0.0 33,811 + -0.7 33,812 - -1.0 33,812 - +0.1 33,812 - +0.5 33,812 - -0.3 33,901 + -0.1 33,902 - -0.4 33,902 - +0.6 33,902 - -0.3 33,902 - -0.4 33,902 - -0.8 33,932 - +0.3 34,378 + -2.0 34,416 + -0.6 34,476 + -0.7 34,477 - +1.0 34,477 - -2.0 34,486 + -0.2 34,487 - -0.5 34,515 + +0.1 34,516 - -0.4 34,516 - -0.6 34,516 - -1.0 34,699 + -1.5 34,699 + +1.5 34,699 + -0.7 34,700 - -1.3 34,700 - -2.0 34,862 - GFF3838 0.10 -0.2 34,904 - GFF3838 0.12 -0.7 34,945 + GFF3838 0.13 -1.6 34,945 + GFF3838 0.13 -0.8 34,945 + GFF3838 0.13 -1.0 34,945 + GFF3838 0.13 -1.5 34,946 - GFF3838 0.13 +0.8 34,946 - GFF3838 0.13 -1.0 34,946 - GFF3838 0.13 -4.0
Or see this region's nucleotide sequence