Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3734 and GFF3735 overlap by 4 nucleotides GFF3735 and GFF3736 are separated by 46 nucleotides GFF3736 and GFF3737 are separated by 85 nucleotides GFF3737 and GFF3738 overlap by 4 nucleotides
GFF3734 - RNA:NAD 2'-phosphotransferase, at 231,596 to 232,132
GFF3734
GFF3735 - 16S rRNA (cytidine(1402)-2'-O)-methyltransferase (EC 2.1.1.198), at 232,129 to 233,076
GFF3735
GFF3736 - UPF0102 protein YraN, at 233,123 to 233,491
GFF3736
GFF3737 - DnaA initiator-associating protein DiaA, at 233,577 to 234,221
GFF3737
GFF3738 - Uncharacterized protein YraP, at 234,218 to 235,030
GFF3738
Position (kb) 233
234 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 232.172 kb on + strand at 232.172 kb on + strand at 232.172 kb on + strand at 232.172 kb on + strand at 232.172 kb on + strand at 232.172 kb on + strand at 232.172 kb on + strand at 232.173 kb on - strand at 232.173 kb on - strand at 232.198 kb on + strand at 232.199 kb on - strand at 232.199 kb on - strand at 232.428 kb on + strand, within GFF3735 at 232.754 kb on + strand, within GFF3735 at 232.754 kb on + strand, within GFF3735 at 232.985 kb on + strand at 232.985 kb on + strand at 232.985 kb on + strand at 232.986 kb on - strand at 232.986 kb on - strand at 232.986 kb on - strand at 232.986 kb on - strand at 232.986 kb on - strand at 232.986 kb on - strand at 233.223 kb on - strand, within GFF3736 at 233.393 kb on + strand, within GFF3736 at 233.393 kb on + strand, within GFF3736 at 233.394 kb on - strand, within GFF3736 at 233.564 kb on - strand at 233.564 kb on - strand at 233.564 kb on - strand at 233.628 kb on + strand at 233.629 kb on - strand at 233.629 kb on - strand at 233.629 kb on - strand at 233.629 kb on - strand at 233.629 kb on - strand at 234.194 kb on - strand at 234.194 kb on - strand at 234.455 kb on + strand, within GFF3738 at 234.455 kb on + strand, within GFF3738 at 234.455 kb on + strand, within GFF3738 at 234.455 kb on + strand, within GFF3738 at 234.455 kb on + strand, within GFF3738 at 234.455 kb on + strand, within GFF3738 at 234.455 kb on + strand, within GFF3738 at 234.455 kb on + strand, within GFF3738 at 234.455 kb on + strand, within GFF3738 at 234.455 kb on + strand, within GFF3738 at 234.455 kb on + strand, within GFF3738 at 234.455 kb on + strand, within GFF3738 at 234.456 kb on - strand, within GFF3738 at 234.456 kb on - strand, within GFF3738 at 234.456 kb on - strand, within GFF3738 at 234.456 kb on - strand, within GFF3738 at 234.456 kb on - strand, within GFF3738 at 234.456 kb on - strand, within GFF3738 at 234.456 kb on - strand, within GFF3738 at 234.456 kb on - strand, within GFF3738 at 234.456 kb on - strand, within GFF3738 at 234.456 kb on - strand, within GFF3738 at 234.456 kb on - strand, within GFF3738 at 234.456 kb on - strand, within GFF3738 at 234.473 kb on + strand, within GFF3738 at 234.473 kb on + strand, within GFF3738 at 234.473 kb on + strand, within GFF3738 at 234.473 kb on + strand, within GFF3738 at 234.474 kb on - strand, within GFF3738 at 234.474 kb on - strand, within GFF3738 at 234.474 kb on - strand, within GFF3738 at 234.474 kb on - strand, within GFF3738
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 232,172 + +1.2 232,172 + +0.4 232,172 + -0.7 232,172 + +0.1 232,172 + +0.3 232,172 + +0.1 232,172 + +0.4 232,173 - -0.2 232,173 - -1.7 232,198 + +0.0 232,199 - -2.2 232,199 - -1.3 232,428 + GFF3735 0.32 -1.8 232,754 + GFF3735 0.66 -0.0 232,754 + GFF3735 0.66 +3.7 232,985 + -2.3 232,985 + -0.5 232,985 + -0.7 232,986 - -1.3 232,986 - +1.7 232,986 - -0.4 232,986 - +0.1 232,986 - -0.7 232,986 - -1.0 233,223 - GFF3736 0.27 -1.2 233,393 + GFF3736 0.73 -0.9 233,393 + GFF3736 0.73 -0.3 233,394 - GFF3736 0.73 -1.1 233,564 - -0.4 233,564 - +0.3 233,564 - -0.5 233,628 + -0.9 233,629 - -0.8 233,629 - -1.1 233,629 - +0.3 233,629 - +0.5 233,629 - -0.1 234,194 - -0.6 234,194 - -0.4 234,455 + GFF3738 0.29 -1.8 234,455 + GFF3738 0.29 -0.3 234,455 + GFF3738 0.29 +0.7 234,455 + GFF3738 0.29 -1.2 234,455 + GFF3738 0.29 -2.9 234,455 + GFF3738 0.29 -0.6 234,455 + GFF3738 0.29 -0.3 234,455 + GFF3738 0.29 -0.2 234,455 + GFF3738 0.29 -1.9 234,455 + GFF3738 0.29 +0.7 234,455 + GFF3738 0.29 -0.7 234,455 + GFF3738 0.29 -0.4 234,456 - GFF3738 0.29 -0.7 234,456 - GFF3738 0.29 -0.5 234,456 - GFF3738 0.29 +1.7 234,456 - GFF3738 0.29 -1.3 234,456 - GFF3738 0.29 -0.3 234,456 - GFF3738 0.29 -0.2 234,456 - GFF3738 0.29 -0.2 234,456 - GFF3738 0.29 -0.3 234,456 - GFF3738 0.29 -0.9 234,456 - GFF3738 0.29 -0.5 234,456 - GFF3738 0.29 -0.1 234,456 - GFF3738 0.29 -0.2 234,473 + GFF3738 0.31 -1.3 234,473 + GFF3738 0.31 -0.5 234,473 + GFF3738 0.31 -1.3 234,473 + GFF3738 0.31 -0.6 234,474 - GFF3738 0.31 -0.2 234,474 - GFF3738 0.31 -0.7 234,474 - GFF3738 0.31 -0.7 234,474 - GFF3738 0.31 -0.3
Or see this region's nucleotide sequence