Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3460 and GFF3461 are separated by 18 nucleotides GFF3461 and GFF3462 overlap by 4 nucleotides GFF3462 and GFF3463 are separated by 4 nucleotides GFF3463 and GFF3464 are separated by 5 nucleotides
GFF3460 - Urea ABC transporter, ATPase protein UrtE, at 282,286 to 282,975
GFF3460
GFF3461 - Urea ABC transporter, ATPase protein UrtD, at 282,994 to 283,794
GFF3461
GFF3462 - Urea ABC transporter, permease protein UrtC, at 283,791 to 284,846
GFF3462
GFF3463 - Urea ABC transporter, permease protein UrtB, at 284,851 to 285,753
GFF3463
GFF3464 - Urea ABC transporter, substrate-binding protein UrtA, at 285,759 to 286,952
GFF3464
Position (kb) 283
284
285 Strain fitness (log2 ratio) -2
-1
0
1
2 at 283.007 kb on + strand at 283.008 kb on - strand at 283.897 kb on + strand, within GFF3462 at 283.930 kb on + strand, within GFF3462 at 284.006 kb on - strand, within GFF3462 at 284.074 kb on + strand, within GFF3462 at 284.074 kb on + strand, within GFF3462 at 284.075 kb on - strand, within GFF3462 at 284.146 kb on + strand, within GFF3462 at 284.147 kb on - strand, within GFF3462 at 284.269 kb on + strand, within GFF3462 at 284.419 kb on + strand, within GFF3462 at 284.420 kb on - strand, within GFF3462 at 284.452 kb on + strand, within GFF3462 at 284.464 kb on + strand, within GFF3462 at 284.465 kb on - strand, within GFF3462 at 284.465 kb on - strand, within GFF3462 at 284.465 kb on - strand, within GFF3462 at 284.465 kb on - strand, within GFF3462 at 284.723 kb on - strand, within GFF3462 at 284.723 kb on - strand, within GFF3462 at 284.723 kb on - strand, within GFF3462 at 284.894 kb on + strand at 284.894 kb on + strand at 284.895 kb on - strand at 284.895 kb on - strand at 284.895 kb on - strand at 284.954 kb on + strand, within GFF3463 at 284.954 kb on + strand, within GFF3463 at 284.955 kb on - strand, within GFF3463 at 285.059 kb on + strand, within GFF3463 at 285.060 kb on - strand, within GFF3463 at 285.060 kb on - strand, within GFF3463 at 285.060 kb on - strand, within GFF3463 at 285.060 kb on - strand, within GFF3463 at 285.060 kb on - strand, within GFF3463 at 285.060 kb on - strand, within GFF3463 at 285.254 kb on + strand, within GFF3463 at 285.398 kb on + strand, within GFF3463 at 285.461 kb on + strand, within GFF3463 at 285.495 kb on - strand, within GFF3463 at 285.545 kb on + strand, within GFF3463 at 285.546 kb on - strand, within GFF3463 at 285.546 kb on - strand, within GFF3463 at 285.596 kb on + strand, within GFF3463 at 285.596 kb on + strand, within GFF3463 at 285.597 kb on - strand, within GFF3463 at 285.605 kb on + strand, within GFF3463 at 285.606 kb on - strand, within GFF3463
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 283,007 + -1.2 283,008 - -1.2 283,897 + GFF3462 0.10 -2.4 283,930 + GFF3462 0.13 +0.6 284,006 - GFF3462 0.20 +0.9 284,074 + GFF3462 0.27 -1.1 284,074 + GFF3462 0.27 +0.5 284,075 - GFF3462 0.27 -0.5 284,146 + GFF3462 0.34 +0.7 284,147 - GFF3462 0.34 -0.6 284,269 + GFF3462 0.45 +0.1 284,419 + GFF3462 0.59 +0.4 284,420 - GFF3462 0.60 -0.4 284,452 + GFF3462 0.63 -0.4 284,464 + GFF3462 0.64 -0.3 284,465 - GFF3462 0.64 -0.3 284,465 - GFF3462 0.64 -0.5 284,465 - GFF3462 0.64 +0.7 284,465 - GFF3462 0.64 -1.4 284,723 - GFF3462 0.88 -0.3 284,723 - GFF3462 0.88 -1.1 284,723 - GFF3462 0.88 -2.0 284,894 + -1.9 284,894 + -0.9 284,895 - +0.5 284,895 - -0.0 284,895 - -0.9 284,954 + GFF3463 0.11 -1.6 284,954 + GFF3463 0.11 -0.1 284,955 - GFF3463 0.12 -0.7 285,059 + GFF3463 0.23 +0.9 285,060 - GFF3463 0.23 -0.4 285,060 - GFF3463 0.23 -0.3 285,060 - GFF3463 0.23 -0.3 285,060 - GFF3463 0.23 -0.1 285,060 - GFF3463 0.23 +0.6 285,060 - GFF3463 0.23 -0.0 285,254 + GFF3463 0.45 +0.1 285,398 + GFF3463 0.61 -0.2 285,461 + GFF3463 0.68 -0.4 285,495 - GFF3463 0.71 +1.2 285,545 + GFF3463 0.77 +0.2 285,546 - GFF3463 0.77 +0.5 285,546 - GFF3463 0.77 +0.4 285,596 + GFF3463 0.83 +0.5 285,596 + GFF3463 0.83 +0.6 285,597 - GFF3463 0.83 -0.6 285,605 + GFF3463 0.83 +2.2 285,606 - GFF3463 0.84 -0.3
Or see this region's nucleotide sequence