Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3414 and GFF3415 are separated by 10 nucleotides GFF3415 and GFF3416 are separated by 41 nucleotides GFF3416 and GFF3417 overlap by 4 nucleotides GFF3417 and GFF3418 overlap by 8 nucleotides
GFF3414 - Fumarylacetoacetase (EC 3.7.1.2), at 237,999 to 239,324
GFF3414
GFF3415 - Cyclase, at 239,335 to 240,141
GFF3415
GFF3416 - Alkanesulfonates transport system permease protein, at 240,183 to 240,962
GFF3416
GFF3417 - Alkanesulfonates transport system permease protein, at 240,959 to 241,729
GFF3417
GFF3418 - ABC transporter, ATP-binding protein (cluster 1, maltose/g3p/polyamine/iron); ABC transporter, ATP-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 241,722 to 242,501
GFF3418
Position (kb) 240
241 Strain fitness (log2 ratio) -2
-1
0
1
2 at 239.627 kb on + strand, within GFF3415 at 239.627 kb on + strand, within GFF3415 at 239.627 kb on + strand, within GFF3415 at 239.627 kb on + strand, within GFF3415 at 239.627 kb on + strand, within GFF3415 at 239.627 kb on + strand, within GFF3415 at 239.627 kb on + strand, within GFF3415 at 239.628 kb on - strand, within GFF3415 at 239.628 kb on - strand, within GFF3415 at 239.628 kb on - strand, within GFF3415 at 239.628 kb on - strand, within GFF3415 at 239.628 kb on - strand, within GFF3415 at 239.628 kb on - strand, within GFF3415 at 239.642 kb on + strand, within GFF3415 at 239.642 kb on + strand, within GFF3415 at 239.642 kb on + strand, within GFF3415 at 239.669 kb on + strand, within GFF3415 at 239.669 kb on + strand, within GFF3415 at 239.670 kb on - strand, within GFF3415 at 239.670 kb on - strand, within GFF3415 at 239.670 kb on - strand, within GFF3415 at 239.685 kb on - strand, within GFF3415 at 239.786 kb on + strand, within GFF3415 at 239.787 kb on - strand, within GFF3415 at 239.787 kb on - strand, within GFF3415 at 239.915 kb on + strand, within GFF3415 at 239.916 kb on - strand, within GFF3415 at 240.035 kb on + strand, within GFF3415 at 240.035 kb on + strand, within GFF3415 at 240.035 kb on + strand, within GFF3415 at 240.035 kb on + strand, within GFF3415 at 240.035 kb on + strand, within GFF3415 at 240.036 kb on - strand, within GFF3415 at 240.036 kb on - strand, within GFF3415 at 240.056 kb on + strand, within GFF3415 at 240.448 kb on + strand, within GFF3416 at 240.449 kb on - strand, within GFF3416 at 240.836 kb on + strand, within GFF3416 at 240.946 kb on + strand at 241.653 kb on + strand at 241.654 kb on - strand at 241.670 kb on + strand at 241.775 kb on - strand at 241.900 kb on + strand, within GFF3418 at 241.900 kb on + strand, within GFF3418 at 241.901 kb on - strand, within GFF3418 at 241.901 kb on - strand, within GFF3418 at 241.940 kb on + strand, within GFF3418
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 239,627 + GFF3415 0.36 -0.6 239,627 + GFF3415 0.36 -0.3 239,627 + GFF3415 0.36 -1.0 239,627 + GFF3415 0.36 -0.3 239,627 + GFF3415 0.36 -1.0 239,627 + GFF3415 0.36 -0.5 239,627 + GFF3415 0.36 +0.5 239,628 - GFF3415 0.36 +0.3 239,628 - GFF3415 0.36 -0.7 239,628 - GFF3415 0.36 -0.2 239,628 - GFF3415 0.36 +0.1 239,628 - GFF3415 0.36 +1.0 239,628 - GFF3415 0.36 -0.1 239,642 + GFF3415 0.38 -0.6 239,642 + GFF3415 0.38 +1.7 239,642 + GFF3415 0.38 +0.1 239,669 + GFF3415 0.41 +0.4 239,669 + GFF3415 0.41 +0.1 239,670 - GFF3415 0.42 -0.6 239,670 - GFF3415 0.42 -0.5 239,670 - GFF3415 0.42 -1.4 239,685 - GFF3415 0.43 -1.1 239,786 + GFF3415 0.56 +0.1 239,787 - GFF3415 0.56 +0.6 239,787 - GFF3415 0.56 +1.4 239,915 + GFF3415 0.72 -0.6 239,916 - GFF3415 0.72 +1.2 240,035 + GFF3415 0.87 -0.4 240,035 + GFF3415 0.87 -0.3 240,035 + GFF3415 0.87 +0.1 240,035 + GFF3415 0.87 -0.6 240,035 + GFF3415 0.87 +0.8 240,036 - GFF3415 0.87 +2.5 240,036 - GFF3415 0.87 -0.3 240,056 + GFF3415 0.89 -0.9 240,448 + GFF3416 0.34 +0.8 240,449 - GFF3416 0.34 +0.3 240,836 + GFF3416 0.84 +2.3 240,946 + -1.4 241,653 + -2.0 241,654 - -0.3 241,670 + -0.2 241,775 - -0.4 241,900 + GFF3418 0.23 +0.1 241,900 + GFF3418 0.23 +0.0 241,901 - GFF3418 0.23 +0.7 241,901 - GFF3418 0.23 +0.8 241,940 + GFF3418 0.28 +1.7
Or see this region's nucleotide sequence