Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3322 and GFF3323 are separated by 181 nucleotides GFF3323 and GFF3324 are separated by 7 nucleotides GFF3324 and GFF3325 are separated by 17 nucleotides GFF3325 and GFF3326 are separated by 8 nucleotides
GFF3322 - hypothetical protein, at 148,892 to 149,752
GFF3322
GFF3323 - Tricarboxylate transport transcriptional regulator TctD, at 149,934 to 150,578
GFF3323
GFF3324 - hypothetical protein, at 150,586 to 151,980
GFF3324
GFF3325 - Uncharacterized protein YaeR with similarity to glyoxylase family, at 151,998 to 152,753
GFF3325
GFF3326 - hypothetical protein, at 152,762 to 153,223
GFF3326
Position (kb) 150
151
152 Strain fitness (log2 ratio) -1
0
1
2 at 149.613 kb on + strand, within GFF3322 at 149.789 kb on + strand at 149.790 kb on - strand at 149.790 kb on - strand at 149.790 kb on - strand at 149.790 kb on - strand at 149.790 kb on - strand at 149.790 kb on - strand at 149.790 kb on - strand at 149.800 kb on + strand at 149.800 kb on + strand at 149.800 kb on + strand at 149.801 kb on - strand at 149.801 kb on - strand at 149.801 kb on - strand at 149.868 kb on + strand at 150.555 kb on + strand at 150.555 kb on + strand at 150.725 kb on - strand at 150.770 kb on - strand, within GFF3324 at 150.871 kb on + strand, within GFF3324 at 150.872 kb on - strand, within GFF3324 at 150.976 kb on + strand, within GFF3324 at 150.976 kb on + strand, within GFF3324 at 150.976 kb on + strand, within GFF3324 at 150.977 kb on - strand, within GFF3324 at 151.106 kb on - strand, within GFF3324 at 151.430 kb on + strand, within GFF3324 at 151.430 kb on + strand, within GFF3324 at 151.430 kb on + strand, within GFF3324 at 151.431 kb on - strand, within GFF3324 at 151.431 kb on - strand, within GFF3324 at 151.431 kb on - strand, within GFF3324 at 151.431 kb on - strand, within GFF3324 at 151.431 kb on - strand, within GFF3324 at 151.657 kb on + strand, within GFF3324 at 151.658 kb on - strand, within GFF3324 at 151.658 kb on - strand, within GFF3324 at 151.658 kb on - strand, within GFF3324 at 151.717 kb on + strand, within GFF3324 at 151.732 kb on - strand, within GFF3324 at 152.122 kb on + strand, within GFF3325 at 152.123 kb on - strand, within GFF3325 at 152.232 kb on + strand, within GFF3325 at 152.233 kb on - strand, within GFF3325 at 152.377 kb on + strand, within GFF3325 at 152.378 kb on - strand, within GFF3325 at 152.378 kb on - strand, within GFF3325 at 152.378 kb on - strand, within GFF3325 at 152.434 kb on + strand, within GFF3325 at 152.434 kb on + strand, within GFF3325 at 152.434 kb on + strand, within GFF3325 at 152.435 kb on - strand, within GFF3325 at 152.686 kb on + strand at 152.687 kb on - strand at 152.687 kb on - strand at 152.708 kb on - strand at 152.820 kb on + strand, within GFF3326 at 152.820 kb on + strand, within GFF3326 at 152.820 kb on + strand, within GFF3326 at 152.821 kb on - strand, within GFF3326 at 152.821 kb on - strand, within GFF3326 at 152.886 kb on + strand, within GFF3326 at 152.887 kb on - strand, within GFF3326 at 152.887 kb on - strand, within GFF3326 at 152.887 kb on - strand, within GFF3326
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 149,613 + GFF3322 0.84 -0.6 149,789 + -0.1 149,790 - +0.5 149,790 - -0.4 149,790 - +1.7 149,790 - -1.1 149,790 - -0.1 149,790 - +0.7 149,790 - +1.3 149,800 + -0.6 149,800 + -0.9 149,800 + +2.7 149,801 - +0.6 149,801 - -0.4 149,801 - +0.4 149,868 + -0.7 150,555 + -0.4 150,555 + +1.1 150,725 - +0.1 150,770 - GFF3324 0.13 +0.1 150,871 + GFF3324 0.20 -0.9 150,872 - GFF3324 0.21 +0.7 150,976 + GFF3324 0.28 +1.1 150,976 + GFF3324 0.28 -0.4 150,976 + GFF3324 0.28 +0.7 150,977 - GFF3324 0.28 +0.3 151,106 - GFF3324 0.37 +0.4 151,430 + GFF3324 0.61 +0.3 151,430 + GFF3324 0.61 +0.3 151,430 + GFF3324 0.61 +0.0 151,431 - GFF3324 0.61 +0.0 151,431 - GFF3324 0.61 +0.0 151,431 - GFF3324 0.61 +0.2 151,431 - GFF3324 0.61 -0.7 151,431 - GFF3324 0.61 -0.1 151,657 + GFF3324 0.77 -0.6 151,658 - GFF3324 0.77 -0.1 151,658 - GFF3324 0.77 +0.3 151,658 - GFF3324 0.77 +0.1 151,717 + GFF3324 0.81 -0.9 151,732 - GFF3324 0.82 +0.5 152,122 + GFF3325 0.16 -0.1 152,123 - GFF3325 0.17 -0.7 152,232 + GFF3325 0.31 +0.7 152,233 - GFF3325 0.31 -0.5 152,377 + GFF3325 0.50 -1.7 152,378 - GFF3325 0.50 -1.6 152,378 - GFF3325 0.50 -0.3 152,378 - GFF3325 0.50 +0.5 152,434 + GFF3325 0.58 -1.4 152,434 + GFF3325 0.58 -0.3 152,434 + GFF3325 0.58 -0.0 152,435 - GFF3325 0.58 +0.2 152,686 + +0.0 152,687 - -0.1 152,687 - -0.7 152,708 - -0.6 152,820 + GFF3326 0.13 -0.4 152,820 + GFF3326 0.13 +0.1 152,820 + GFF3326 0.13 +0.2 152,821 - GFF3326 0.13 -0.1 152,821 - GFF3326 0.13 +0.3 152,886 + GFF3326 0.27 +0.3 152,887 - GFF3326 0.27 -1.6 152,887 - GFF3326 0.27 -0.6 152,887 - GFF3326 0.27 +0.2
Or see this region's nucleotide sequence