Strain Fitness in Variovorax sp. SCN45 around GFF323

Experiment: M9 with Supernatant from Cyptococcus

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF322 and GFF323 overlap by 4 nucleotidesGFF323 and GFF324 are separated by 38 nucleotidesGFF324 and GFF325 are separated by 154 nucleotides GFF322 - GTP cyclohydrolase II homolog, at 354,009 to 355,151 GFF322 GFF323 - Possible pyrimidine-degrading protein DUF1688, at 355,148 to 356,554 GFF323 GFF324 - Uracil phosphoribosyltransferase (EC 2.4.2.9), at 356,593 to 357,222 GFF324 GFF325 - Nucleoside ABC transporter, substrate-binding protein, at 357,377 to 358,381 GFF325 Position (kb) 355 356 357Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 354.261 kb on + strand, within GFF322at 354.261 kb on + strand, within GFF322at 354.417 kb on + strand, within GFF322at 354.418 kb on - strand, within GFF322at 354.498 kb on + strand, within GFF322at 354.498 kb on + strand, within GFF322at 354.498 kb on + strand, within GFF322at 354.499 kb on - strand, within GFF322at 354.499 kb on - strand, within GFF322at 354.499 kb on - strand, within GFF322at 354.499 kb on - strand, within GFF322at 354.564 kb on + strand, within GFF322at 354.564 kb on + strand, within GFF322at 354.564 kb on + strand, within GFF322at 354.564 kb on + strand, within GFF322at 354.564 kb on + strand, within GFF322at 354.564 kb on + strand, within GFF322at 354.565 kb on - strand, within GFF322at 354.565 kb on - strand, within GFF322at 354.565 kb on - strand, within GFF322at 354.565 kb on - strand, within GFF322at 354.565 kb on - strand, within GFF322at 354.681 kb on + strand, within GFF322at 354.681 kb on + strand, within GFF322at 354.682 kb on - strand, within GFF322at 354.798 kb on + strand, within GFF322at 354.798 kb on + strand, within GFF322at 354.798 kb on + strand, within GFF322at 354.799 kb on - strand, within GFF322at 354.799 kb on - strand, within GFF322at 354.799 kb on - strand, within GFF322at 354.843 kb on + strand, within GFF322at 354.844 kb on - strand, within GFF322at 355.080 kb on + strandat 355.081 kb on - strandat 355.081 kb on - strandat 355.242 kb on - strandat 355.242 kb on - strandat 355.613 kb on + strand, within GFF323at 355.613 kb on + strand, within GFF323at 355.613 kb on + strand, within GFF323at 355.614 kb on - strand, within GFF323at 355.674 kb on - strand, within GFF323at 355.674 kb on - strand, within GFF323at 355.674 kb on - strand, within GFF323at 355.674 kb on - strand, within GFF323at 355.674 kb on - strand, within GFF323at 355.722 kb on - strand, within GFF323at 355.722 kb on - strand, within GFF323at 356.183 kb on + strand, within GFF323at 356.184 kb on - strand, within GFF323at 356.259 kb on - strand, within GFF323at 356.506 kb on + strandat 356.506 kb on + strandat 356.552 kb on + strandat 356.553 kb on - strandat 356.579 kb on + strandat 356.579 kb on + strandat 356.580 kb on - strandat 356.580 kb on - strandat 356.716 kb on + strand, within GFF324at 356.716 kb on + strand, within GFF324at 356.716 kb on + strand, within GFF324at 356.717 kb on - strand, within GFF324at 356.717 kb on - strand, within GFF324at 356.717 kb on - strand, within GFF324at 356.717 kb on - strand, within GFF324at 356.899 kb on + strand, within GFF324at 356.900 kb on - strand, within GFF324at 356.932 kb on + strand, within GFF324at 356.932 kb on + strand, within GFF324at 356.933 kb on - strand, within GFF324at 356.933 kb on - strand, within GFF324at 356.933 kb on - strand, within GFF324at 356.933 kb on - strand, within GFF324at 356.933 kb on - strand, within GFF324at 356.935 kb on + strand, within GFF324at 356.935 kb on + strand, within GFF324at 356.935 kb on + strand, within GFF324at 356.935 kb on + strand, within GFF324at 356.935 kb on + strand, within GFF324at 356.935 kb on + strand, within GFF324at 356.935 kb on + strand, within GFF324at 356.935 kb on + strand, within GFF324at 356.935 kb on + strand, within GFF324at 356.935 kb on + strand, within GFF324at 356.936 kb on - strand, within GFF324at 356.936 kb on - strand, within GFF324at 356.936 kb on - strand, within GFF324at 356.936 kb on - strand, within GFF324at 356.936 kb on - strand, within GFF324at 356.936 kb on - strand, within GFF324at 356.936 kb on - strand, within GFF324at 357.130 kb on + strand, within GFF324at 357.130 kb on + strand, within GFF324at 357.130 kb on + strand, within GFF324at 357.130 kb on + strand, within GFF324at 357.130 kb on + strand, within GFF324at 357.131 kb on - strand, within GFF324at 357.131 kb on - strand, within GFF324at 357.131 kb on - strand, within GFF324at 357.169 kb on + strandat 357.169 kb on + strandat 357.170 kb on - strandat 357.170 kb on - strandat 357.170 kb on - strandat 357.170 kb on - strandat 357.221 kb on - strandat 357.221 kb on - strandat 357.359 kb on - strandat 357.491 kb on + strand, within GFF325at 357.491 kb on + strand, within GFF325at 357.491 kb on + strand, within GFF325at 357.491 kb on + strand, within GFF325at 357.491 kb on + strand, within GFF325at 357.491 kb on + strand, within GFF325at 357.491 kb on + strand, within GFF325at 357.491 kb on + strand, within GFF325at 357.491 kb on + strand, within GFF325at 357.492 kb on - strand, within GFF325at 357.492 kb on - strand, within GFF325at 357.492 kb on - strand, within GFF325at 357.492 kb on - strand, within GFF325at 357.492 kb on - strand, within GFF325at 357.492 kb on - strand, within GFF325at 357.492 kb on - strand, within GFF325at 357.492 kb on - strand, within GFF325at 357.492 kb on - strand, within GFF325

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus
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354,261 + GFF322 0.22 +0.6
354,261 + GFF322 0.22 +0.0
354,417 + GFF322 0.36 -0.3
354,418 - GFF322 0.36 -1.3
354,498 + GFF322 0.43 -0.5
354,498 + GFF322 0.43 -0.3
354,498 + GFF322 0.43 -0.9
354,499 - GFF322 0.43 +0.3
354,499 - GFF322 0.43 +0.0
354,499 - GFF322 0.43 -2.2
354,499 - GFF322 0.43 -1.5
354,564 + GFF322 0.49 -1.9
354,564 + GFF322 0.49 -0.1
354,564 + GFF322 0.49 -0.1
354,564 + GFF322 0.49 +0.3
354,564 + GFF322 0.49 +0.1
354,564 + GFF322 0.49 +0.3
354,565 - GFF322 0.49 +0.2
354,565 - GFF322 0.49 -1.2
354,565 - GFF322 0.49 -1.5
354,565 - GFF322 0.49 -0.6
354,565 - GFF322 0.49 +0.2
354,681 + GFF322 0.59 -0.1
354,681 + GFF322 0.59 +0.1
354,682 - GFF322 0.59 +0.0
354,798 + GFF322 0.69 -0.8
354,798 + GFF322 0.69 -0.1
354,798 + GFF322 0.69 -0.4
354,799 - GFF322 0.69 -0.4
354,799 - GFF322 0.69 +0.2
354,799 - GFF322 0.69 +0.1
354,843 + GFF322 0.73 -1.5
354,844 - GFF322 0.73 +0.1
355,080 + -1.5
355,081 - -1.3
355,081 - +0.2
355,242 - +0.1
355,242 - -1.3
355,613 + GFF323 0.33 -1.5
355,613 + GFF323 0.33 +0.0
355,613 + GFF323 0.33 -0.2
355,614 - GFF323 0.33 -0.6
355,674 - GFF323 0.37 +0.0
355,674 - GFF323 0.37 -0.7
355,674 - GFF323 0.37 -0.1
355,674 - GFF323 0.37 -0.5
355,674 - GFF323 0.37 -0.9
355,722 - GFF323 0.41 +0.4
355,722 - GFF323 0.41 +0.9
356,183 + GFF323 0.74 -0.6
356,184 - GFF323 0.74 -0.8
356,259 - GFF323 0.79 +0.9
356,506 + -0.6
356,506 + +0.3
356,552 + -0.7
356,553 - +0.5
356,579 + -0.9
356,579 + -1.1
356,580 - -0.3
356,580 - +0.8
356,716 + GFF324 0.20 -0.4
356,716 + GFF324 0.20 -0.5
356,716 + GFF324 0.20 -0.1
356,717 - GFF324 0.20 -0.6
356,717 - GFF324 0.20 -1.2
356,717 - GFF324 0.20 -0.2
356,717 - GFF324 0.20 -0.8
356,899 + GFF324 0.49 -0.8
356,900 - GFF324 0.49 +0.0
356,932 + GFF324 0.54 -0.1
356,932 + GFF324 0.54 +0.9
356,933 - GFF324 0.54 +0.7
356,933 - GFF324 0.54 -0.6
356,933 - GFF324 0.54 -0.6
356,933 - GFF324 0.54 -2.7
356,933 - GFF324 0.54 -0.8
356,935 + GFF324 0.54 -2.2
356,935 + GFF324 0.54 -0.1
356,935 + GFF324 0.54 +2.0
356,935 + GFF324 0.54 +0.2
356,935 + GFF324 0.54 +0.2
356,935 + GFF324 0.54 +0.4
356,935 + GFF324 0.54 -0.5
356,935 + GFF324 0.54 -0.4
356,935 + GFF324 0.54 -0.7
356,935 + GFF324 0.54 -0.1
356,936 - GFF324 0.54 -0.4
356,936 - GFF324 0.54 +0.5
356,936 - GFF324 0.54 +1.6
356,936 - GFF324 0.54 -0.3
356,936 - GFF324 0.54 +0.0
356,936 - GFF324 0.54 -0.1
356,936 - GFF324 0.54 -1.2
357,130 + GFF324 0.85 -1.7
357,130 + GFF324 0.85 -0.3
357,130 + GFF324 0.85 -0.3
357,130 + GFF324 0.85 +0.0
357,130 + GFF324 0.85 -0.1
357,131 - GFF324 0.85 -0.4
357,131 - GFF324 0.85 -0.2
357,131 - GFF324 0.85 +0.1
357,169 + -1.1
357,169 + -0.7
357,170 - +1.9
357,170 - +1.3
357,170 - +0.3
357,170 - +1.8
357,221 - -0.3
357,221 - -3.2
357,359 - -0.9
357,491 + GFF325 0.11 +1.0
357,491 + GFF325 0.11 +0.3
357,491 + GFF325 0.11 -0.1
357,491 + GFF325 0.11 +0.2
357,491 + GFF325 0.11 -0.6
357,491 + GFF325 0.11 -0.1
357,491 + GFF325 0.11 +0.3
357,491 + GFF325 0.11 +0.9
357,491 + GFF325 0.11 -0.1
357,492 - GFF325 0.11 +0.4
357,492 - GFF325 0.11 -0.1
357,492 - GFF325 0.11 -0.0
357,492 - GFF325 0.11 -1.3
357,492 - GFF325 0.11 +0.2
357,492 - GFF325 0.11 -0.8
357,492 - GFF325 0.11 -1.3
357,492 - GFF325 0.11 -0.9
357,492 - GFF325 0.11 -0.9

Or see this region's nucleotide sequence