Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3033 and GFF3034 are separated by 74 nucleotides GFF3034 and GFF3035 are separated by 96 nucleotides GFF3035 and GFF3036 are separated by 9 nucleotides GFF3036 and GFF3037 are separated by 6 nucleotides GFF3037 and GFF3038 are separated by 11 nucleotides
GFF3033 - hypothetical protein, at 465,132 to 466,934
GFF3033
GFF3034 - no description, at 467,009 to 467,281
GFF3034
GFF3035 - Ferrichrome-iron receptor, at 467,378 to 469,573
GFF3035
GFF3036 - no description, at 469,583 to 470,005
GFF3036
GFF3037 - no description, at 470,012 to 470,479
GFF3037
GFF3038 - hypothetical protein, at 470,491 to 471,174
GFF3038
Position (kb) 467
468
469
470 Strain fitness (log2 ratio) -2
-1
0
1
2 at 466.431 kb on + strand, within GFF3033 at 466.431 kb on + strand, within GFF3033 at 466.431 kb on + strand, within GFF3033 at 467.033 kb on + strand at 467.034 kb on - strand at 467.034 kb on - strand at 467.034 kb on - strand at 467.034 kb on - strand at 467.229 kb on - strand, within GFF3034 at 467.789 kb on + strand, within GFF3035 at 467.789 kb on + strand, within GFF3035 at 467.789 kb on + strand, within GFF3035 at 467.789 kb on + strand, within GFF3035 at 467.789 kb on + strand, within GFF3035 at 467.789 kb on + strand, within GFF3035 at 467.789 kb on + strand, within GFF3035 at 467.790 kb on - strand, within GFF3035 at 467.790 kb on - strand, within GFF3035 at 467.790 kb on - strand, within GFF3035 at 467.790 kb on - strand, within GFF3035 at 467.790 kb on - strand, within GFF3035 at 467.790 kb on - strand, within GFF3035 at 467.790 kb on - strand, within GFF3035 at 467.862 kb on - strand, within GFF3035 at 467.963 kb on + strand, within GFF3035 at 467.964 kb on - strand, within GFF3035 at 468.122 kb on + strand, within GFF3035 at 468.122 kb on + strand, within GFF3035 at 468.123 kb on - strand, within GFF3035 at 468.251 kb on + strand, within GFF3035 at 468.263 kb on + strand, within GFF3035 at 468.353 kb on + strand, within GFF3035 at 468.407 kb on + strand, within GFF3035 at 468.408 kb on - strand, within GFF3035 at 468.453 kb on - strand, within GFF3035 at 468.564 kb on - strand, within GFF3035 at 468.614 kb on + strand, within GFF3035 at 468.632 kb on + strand, within GFF3035 at 468.847 kb on + strand, within GFF3035 at 468.848 kb on - strand, within GFF3035 at 468.848 kb on - strand, within GFF3035 at 468.851 kb on + strand, within GFF3035 at 468.920 kb on + strand, within GFF3035 at 468.929 kb on + strand, within GFF3035 at 468.929 kb on + strand, within GFF3035 at 468.930 kb on - strand, within GFF3035 at 468.930 kb on - strand, within GFF3035 at 468.930 kb on - strand, within GFF3035 at 469.016 kb on + strand, within GFF3035 at 469.095 kb on - strand, within GFF3035 at 469.139 kb on + strand, within GFF3035 at 469.139 kb on + strand, within GFF3035 at 469.241 kb on + strand, within GFF3035 at 469.242 kb on - strand, within GFF3035 at 469.242 kb on - strand, within GFF3035 at 469.299 kb on - strand, within GFF3035 at 469.346 kb on + strand, within GFF3035 at 469.347 kb on - strand, within GFF3035 at 469.425 kb on - strand at 469.517 kb on + strand at 469.517 kb on + strand at 469.571 kb on + strand at 469.710 kb on - strand, within GFF3036 at 469.721 kb on + strand, within GFF3036 at 469.743 kb on - strand, within GFF3036 at 469.743 kb on - strand, within GFF3036 at 469.743 kb on - strand, within GFF3036 at 469.895 kb on + strand, within GFF3036 at 469.896 kb on - strand, within GFF3036 at 470.351 kb on + strand, within GFF3037 at 470.351 kb on + strand, within GFF3037 at 470.351 kb on + strand, within GFF3037 at 470.352 kb on - strand, within GFF3037
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 466,431 + GFF3033 0.72 +0.1 466,431 + GFF3033 0.72 -2.1 466,431 + GFF3033 0.72 -1.3 467,033 + -0.6 467,034 - -0.6 467,034 - -0.3 467,034 - -0.4 467,034 - -0.5 467,229 - GFF3034 0.81 -0.5 467,789 + GFF3035 0.19 -1.3 467,789 + GFF3035 0.19 -0.2 467,789 + GFF3035 0.19 -0.3 467,789 + GFF3035 0.19 +0.5 467,789 + GFF3035 0.19 -0.4 467,789 + GFF3035 0.19 -0.7 467,789 + GFF3035 0.19 -0.5 467,790 - GFF3035 0.19 -0.7 467,790 - GFF3035 0.19 +0.1 467,790 - GFF3035 0.19 -0.3 467,790 - GFF3035 0.19 -0.4 467,790 - GFF3035 0.19 -0.2 467,790 - GFF3035 0.19 +0.7 467,790 - GFF3035 0.19 -1.1 467,862 - GFF3035 0.22 +0.4 467,963 + GFF3035 0.27 +0.3 467,964 - GFF3035 0.27 -0.9 468,122 + GFF3035 0.34 -1.2 468,122 + GFF3035 0.34 -1.5 468,123 - GFF3035 0.34 -0.6 468,251 + GFF3035 0.40 +1.4 468,263 + GFF3035 0.40 +0.7 468,353 + GFF3035 0.44 -0.5 468,407 + GFF3035 0.47 -0.1 468,408 - GFF3035 0.47 -0.3 468,453 - GFF3035 0.49 +0.5 468,564 - GFF3035 0.54 -0.2 468,614 + GFF3035 0.56 -0.1 468,632 + GFF3035 0.57 -0.2 468,847 + GFF3035 0.67 -0.3 468,848 - GFF3035 0.67 +0.7 468,848 - GFF3035 0.67 -0.2 468,851 + GFF3035 0.67 -0.2 468,920 + GFF3035 0.70 +0.4 468,929 + GFF3035 0.71 +1.2 468,929 + GFF3035 0.71 -0.2 468,930 - GFF3035 0.71 -1.1 468,930 - GFF3035 0.71 +0.1 468,930 - GFF3035 0.71 -0.2 469,016 + GFF3035 0.75 +2.0 469,095 - GFF3035 0.78 +2.1 469,139 + GFF3035 0.80 -0.6 469,139 + GFF3035 0.80 -0.4 469,241 + GFF3035 0.85 +1.8 469,242 - GFF3035 0.85 +0.0 469,242 - GFF3035 0.85 +0.7 469,299 - GFF3035 0.87 +1.2 469,346 + GFF3035 0.90 +0.3 469,347 - GFF3035 0.90 -1.0 469,425 - +0.2 469,517 + +0.2 469,517 + -0.3 469,571 + -1.1 469,710 - GFF3036 0.30 +0.7 469,721 + GFF3036 0.33 -0.3 469,743 - GFF3036 0.38 +0.1 469,743 - GFF3036 0.38 +0.7 469,743 - GFF3036 0.38 -0.1 469,895 + GFF3036 0.74 +0.2 469,896 - GFF3036 0.74 -0.7 470,351 + GFF3037 0.72 +0.3 470,351 + GFF3037 0.72 +0.0 470,351 + GFF3037 0.72 -1.4 470,352 - GFF3037 0.73 -0.7
Or see this region's nucleotide sequence