Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3003 and GFF3004 are separated by 6 nucleotides GFF3004 and GFF3005 are separated by 5 nucleotides GFF3005 and GFF3006 overlap by 4 nucleotides
GFF3003 - Cobalt-containing nitrile hydratase subunit alpha (EC 4.2.1.84), at 432,323 to 432,952
GFF3003
GFF3004 - Thymidylate synthase (EC 2.1.1.45), at 432,959 to 433,828
GFF3004
GFF3005 - Dihydrofolate reductase (EC 1.5.1.3), at 433,834 to 434,355
GFF3005
GFF3006 - hypothetical protein, at 434,352 to 435,473
GFF3006
Position (kb) 433
434
435 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 432.858 kb on + strand, within GFF3003 at 432.858 kb on + strand, within GFF3003 at 432.858 kb on + strand, within GFF3003 at 432.859 kb on - strand, within GFF3003 at 432.859 kb on - strand, within GFF3003 at 432.859 kb on - strand, within GFF3003 at 432.859 kb on - strand, within GFF3003 at 432.859 kb on - strand, within GFF3003 at 432.875 kb on + strand, within GFF3003 at 432.876 kb on - strand, within GFF3003 at 432.876 kb on - strand, within GFF3003 at 432.876 kb on - strand, within GFF3003 at 432.958 kb on + strand at 432.958 kb on + strand at 432.958 kb on + strand at 432.959 kb on - strand at 432.959 kb on - strand at 432.959 kb on - strand at 432.959 kb on - strand at 432.959 kb on - strand at 432.959 kb on - strand at 432.963 kb on + strand at 432.963 kb on + strand at 432.964 kb on - strand at 434.610 kb on + strand, within GFF3006 at 434.611 kb on - strand, within GFF3006 at 434.611 kb on - strand, within GFF3006 at 435.063 kb on + strand, within GFF3006 at 435.063 kb on + strand, within GFF3006 at 435.064 kb on - strand, within GFF3006 at 435.064 kb on - strand, within GFF3006 at 435.082 kb on + strand, within GFF3006 at 435.082 kb on + strand, within GFF3006 at 435.082 kb on + strand, within GFF3006 at 435.082 kb on + strand, within GFF3006 at 435.083 kb on - strand, within GFF3006 at 435.083 kb on - strand, within GFF3006 at 435.083 kb on - strand, within GFF3006 at 435.083 kb on - strand, within GFF3006 at 435.090 kb on + strand, within GFF3006 at 435.297 kb on + strand, within GFF3006 at 435.297 kb on + strand, within GFF3006 at 435.297 kb on + strand, within GFF3006 at 435.297 kb on + strand, within GFF3006 at 435.298 kb on - strand, within GFF3006 at 435.298 kb on - strand, within GFF3006 at 435.298 kb on - strand, within GFF3006
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 432,858 + GFF3003 0.85 +0.2 432,858 + GFF3003 0.85 -0.6 432,858 + GFF3003 0.85 +0.7 432,859 - GFF3003 0.85 -0.5 432,859 - GFF3003 0.85 +0.1 432,859 - GFF3003 0.85 -0.7 432,859 - GFF3003 0.85 +0.5 432,859 - GFF3003 0.85 -0.0 432,875 + GFF3003 0.88 +0.9 432,876 - GFF3003 0.88 -0.1 432,876 - GFF3003 0.88 +1.5 432,876 - GFF3003 0.88 +1.2 432,958 + -0.1 432,958 + -1.8 432,958 + +0.5 432,959 - -0.5 432,959 - +0.6 432,959 - +1.2 432,959 - +0.3 432,959 - +0.2 432,959 - -0.2 432,963 + -0.4 432,963 + -0.3 432,964 - +0.1 434,610 + GFF3006 0.23 +0.0 434,611 - GFF3006 0.23 -0.4 434,611 - GFF3006 0.23 +0.8 435,063 + GFF3006 0.63 -0.4 435,063 + GFF3006 0.63 -1.8 435,064 - GFF3006 0.63 -0.4 435,064 - GFF3006 0.63 -2.7 435,082 + GFF3006 0.65 +0.6 435,082 + GFF3006 0.65 +0.5 435,082 + GFF3006 0.65 +0.3 435,082 + GFF3006 0.65 -1.8 435,083 - GFF3006 0.65 -0.4 435,083 - GFF3006 0.65 -0.2 435,083 - GFF3006 0.65 -0.1 435,083 - GFF3006 0.65 -0.5 435,090 + GFF3006 0.66 -1.1 435,297 + GFF3006 0.84 -0.5 435,297 + GFF3006 0.84 -0.3 435,297 + GFF3006 0.84 +0.7 435,297 + GFF3006 0.84 -1.3 435,298 - GFF3006 0.84 -0.4 435,298 - GFF3006 0.84 +3.3 435,298 - GFF3006 0.84 +1.7
Or see this region's nucleotide sequence