Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3001 and GFF3002 overlap by 4 nucleotides GFF3002 and GFF3003 overlap by 4 nucleotides GFF3003 and GFF3004 are separated by 6 nucleotides GFF3004 and GFF3005 are separated by 5 nucleotides
GFF3001 - Metal chaperone, involved in Zn homeostasis, at 431,296 to 431,664
GFF3001
GFF3002 - Cobalt-containing nitrile hydratase subunit beta (EC 4.2.1.84), at 431,661 to 432,326
GFF3002
GFF3003 - Cobalt-containing nitrile hydratase subunit alpha (EC 4.2.1.84), at 432,323 to 432,952
GFF3003
GFF3004 - Thymidylate synthase (EC 2.1.1.45), at 432,959 to 433,828
GFF3004
GFF3005 - Dihydrofolate reductase (EC 1.5.1.3), at 433,834 to 434,355
GFF3005
Position (kb)
432
433 Strain fitness (log2 ratio)
-2
-1
0
1 at 431.379 kb on - strand, within GFF3001 at 431.453 kb on + strand, within GFF3001 at 431.453 kb on + strand, within GFF3001 at 431.454 kb on - strand, within GFF3001 at 431.454 kb on - strand, within GFF3001 at 431.454 kb on - strand, within GFF3001 at 431.454 kb on - strand, within GFF3001 at 431.454 kb on - strand, within GFF3001 at 431.456 kb on + strand, within GFF3001 at 431.456 kb on + strand, within GFF3001 at 431.456 kb on + strand, within GFF3001 at 431.456 kb on + strand, within GFF3001 at 431.457 kb on - strand, within GFF3001 at 431.457 kb on - strand, within GFF3001 at 431.457 kb on - strand, within GFF3001 at 431.680 kb on + strand at 431.681 kb on - strand at 431.681 kb on - strand at 431.767 kb on + strand, within GFF3002 at 431.767 kb on + strand, within GFF3002 at 431.767 kb on + strand, within GFF3002 at 431.767 kb on + strand, within GFF3002 at 431.767 kb on + strand, within GFF3002 at 431.768 kb on - strand, within GFF3002 at 431.768 kb on - strand, within GFF3002 at 431.768 kb on - strand, within GFF3002 at 431.768 kb on - strand, within GFF3002 at 431.768 kb on - strand, within GFF3002 at 431.768 kb on - strand, within GFF3002 at 431.867 kb on - strand, within GFF3002 at 431.867 kb on - strand, within GFF3002 at 431.867 kb on - strand, within GFF3002 at 432.025 kb on - strand, within GFF3002 at 432.025 kb on - strand, within GFF3002 at 432.025 kb on - strand, within GFF3002 at 432.121 kb on + strand, within GFF3002 at 432.121 kb on + strand, within GFF3002 at 432.124 kb on + strand, within GFF3002 at 432.124 kb on + strand, within GFF3002 at 432.124 kb on + strand, within GFF3002 at 432.124 kb on + strand, within GFF3002 at 432.124 kb on + strand, within GFF3002 at 432.125 kb on - strand, within GFF3002 at 432.125 kb on - strand, within GFF3002 at 432.125 kb on - strand, within GFF3002 at 432.125 kb on - strand, within GFF3002 at 432.125 kb on - strand, within GFF3002 at 432.125 kb on - strand, within GFF3002 at 432.125 kb on - strand, within GFF3002 at 432.125 kb on - strand, within GFF3002 at 432.125 kb on - strand, within GFF3002 at 432.125 kb on - strand, within GFF3002 at 432.125 kb on - strand, within GFF3002 at 432.140 kb on - strand, within GFF3002 at 432.319 kb on + strand at 432.319 kb on + strand at 432.319 kb on + strand at 432.319 kb on + strand at 432.319 kb on + strand at 432.319 kb on + strand at 432.319 kb on + strand at 432.319 kb on + strand at 432.319 kb on + strand at 432.320 kb on - strand at 432.320 kb on - strand at 432.320 kb on - strand at 432.320 kb on - strand at 432.320 kb on - strand at 432.320 kb on - strand at 432.320 kb on - strand at 432.457 kb on - strand, within GFF3003 at 432.579 kb on + strand, within GFF3003 at 432.580 kb on - strand, within GFF3003 at 432.580 kb on - strand, within GFF3003 at 432.580 kb on - strand, within GFF3003 at 432.702 kb on + strand, within GFF3003 at 432.819 kb on + strand, within GFF3003 at 432.819 kb on + strand, within GFF3003 at 432.820 kb on - strand, within GFF3003 at 432.820 kb on - strand, within GFF3003 at 432.820 kb on - strand, within GFF3003 at 432.820 kb on - strand, within GFF3003 at 432.820 kb on - strand, within GFF3003 at 432.820 kb on - strand, within GFF3003 at 432.820 kb on - strand, within GFF3003 at 432.858 kb on + strand, within GFF3003 at 432.858 kb on + strand, within GFF3003 at 432.858 kb on + strand, within GFF3003 at 432.859 kb on - strand, within GFF3003 at 432.859 kb on - strand, within GFF3003 at 432.859 kb on - strand, within GFF3003 at 432.859 kb on - strand, within GFF3003 at 432.859 kb on - strand, within GFF3003 at 432.875 kb on + strand, within GFF3003 at 432.876 kb on - strand, within GFF3003 at 432.876 kb on - strand, within GFF3003 at 432.876 kb on - strand, within GFF3003 at 432.958 kb on + strand at 432.958 kb on + strand at 432.958 kb on + strand at 432.959 kb on - strand at 432.959 kb on - strand at 432.959 kb on - strand at 432.959 kb on - strand at 432.959 kb on - strand at 432.959 kb on - strand at 432.963 kb on + strand at 432.963 kb on + strand at 432.964 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 431,379 - GFF3001 0.22 +0.4 431,453 + GFF3001 0.43 +1.0 431,453 + GFF3001 0.43 +0.2 431,454 - GFF3001 0.43 -1.1 431,454 - GFF3001 0.43 -0.3 431,454 - GFF3001 0.43 -1.3 431,454 - GFF3001 0.43 -0.5 431,454 - GFF3001 0.43 +0.2 431,456 + GFF3001 0.43 +0.9 431,456 + GFF3001 0.43 +0.2 431,456 + GFF3001 0.43 -0.5 431,456 + GFF3001 0.43 +0.3 431,457 - GFF3001 0.44 -0.8 431,457 - GFF3001 0.44 -0.2 431,457 - GFF3001 0.44 -2.6 431,680 + +0.3 431,681 - -0.2 431,681 - -0.2 431,767 + GFF3002 0.16 +0.3 431,767 + GFF3002 0.16 -1.6 431,767 + GFF3002 0.16 +0.2 431,767 + GFF3002 0.16 -0.5 431,767 + GFF3002 0.16 -0.7 431,768 - GFF3002 0.16 -0.2 431,768 - GFF3002 0.16 +0.0 431,768 - GFF3002 0.16 -0.8 431,768 - GFF3002 0.16 +0.2 431,768 - GFF3002 0.16 +1.0 431,768 - GFF3002 0.16 -0.4 431,867 - GFF3002 0.31 +1.3 431,867 - GFF3002 0.31 -0.6 431,867 - GFF3002 0.31 +0.3 432,025 - GFF3002 0.55 -0.4 432,025 - GFF3002 0.55 -2.4 432,025 - GFF3002 0.55 -1.5 432,121 + GFF3002 0.69 +0.7 432,121 + GFF3002 0.69 -0.5 432,124 + GFF3002 0.70 +0.4 432,124 + GFF3002 0.70 -0.9 432,124 + GFF3002 0.70 -1.1 432,124 + GFF3002 0.70 -0.7 432,124 + GFF3002 0.70 -0.3 432,125 - GFF3002 0.70 -1.1 432,125 - GFF3002 0.70 +1.0 432,125 - GFF3002 0.70 -0.3 432,125 - GFF3002 0.70 +0.1 432,125 - GFF3002 0.70 +0.4 432,125 - GFF3002 0.70 -0.3 432,125 - GFF3002 0.70 -0.1 432,125 - GFF3002 0.70 -0.4 432,125 - GFF3002 0.70 -2.2 432,125 - GFF3002 0.70 -0.0 432,125 - GFF3002 0.70 +0.7 432,140 - GFF3002 0.72 -0.2 432,319 + -2.6 432,319 + -0.1 432,319 + +0.7 432,319 + +1.1 432,319 + -0.2 432,319 + +0.2 432,319 + -0.5 432,319 + -0.4 432,319 + -0.5 432,320 - -0.3 432,320 - -0.8 432,320 - +0.0 432,320 - +0.7 432,320 - -0.5 432,320 - -0.4 432,320 - -0.1 432,457 - GFF3003 0.21 +0.9 432,579 + GFF3003 0.41 -0.8 432,580 - GFF3003 0.41 +1.0 432,580 - GFF3003 0.41 +1.5 432,580 - GFF3003 0.41 -0.5 432,702 + GFF3003 0.60 -0.1 432,819 + GFF3003 0.79 +1.1 432,819 + GFF3003 0.79 -1.4 432,820 - GFF3003 0.79 -0.6 432,820 - GFF3003 0.79 +0.3 432,820 - GFF3003 0.79 -0.1 432,820 - GFF3003 0.79 -0.0 432,820 - GFF3003 0.79 +1.2 432,820 - GFF3003 0.79 -0.5 432,820 - GFF3003 0.79 -0.8 432,858 + GFF3003 0.85 +0.2 432,858 + GFF3003 0.85 -0.6 432,858 + GFF3003 0.85 +0.7 432,859 - GFF3003 0.85 -0.5 432,859 - GFF3003 0.85 +0.1 432,859 - GFF3003 0.85 -0.7 432,859 - GFF3003 0.85 +0.5 432,859 - GFF3003 0.85 -0.0 432,875 + GFF3003 0.88 +0.9 432,876 - GFF3003 0.88 -0.1 432,876 - GFF3003 0.88 +1.5 432,876 - GFF3003 0.88 +1.2 432,958 + -0.1 432,958 + -1.8 432,958 + +0.5 432,959 - -0.5 432,959 - +0.6 432,959 - +1.2 432,959 - +0.3 432,959 - +0.2 432,959 - -0.2 432,963 + -0.4 432,963 + -0.3 432,964 - +0.1
Or see this region's nucleotide sequence