Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2988 and GFF2989 are separated by 8 nucleotides GFF2989 and GFF2990 are separated by 50 nucleotides GFF2990 and GFF2991 are separated by 18 nucleotides
GFF2988 - O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) @ O-succinylhomoserine sulfhydrylase (EC 2.5.1.48), at 418,335 to 419,639
GFF2988
GFF2989 - hypothetical protein, at 419,648 to 421,054
GFF2989
GFF2990 - Probable hydrolase protein (EC 3.-.-.-), at 421,105 to 421,926
GFF2990
GFF2991 - BUG/TctC family periplasmic protein, at 421,945 to 422,904
GFF2991
Position (kb) 419
420
421
422 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 418.671 kb on + strand, within GFF2988 at 418.698 kb on + strand, within GFF2988 at 418.698 kb on + strand, within GFF2988 at 418.698 kb on + strand, within GFF2988 at 418.698 kb on + strand, within GFF2988 at 418.699 kb on - strand, within GFF2988 at 418.908 kb on + strand, within GFF2988 at 418.908 kb on + strand, within GFF2988 at 418.908 kb on + strand, within GFF2988 at 418.908 kb on + strand, within GFF2988 at 418.908 kb on + strand, within GFF2988 at 418.908 kb on + strand, within GFF2988 at 418.909 kb on - strand, within GFF2988 at 418.947 kb on + strand, within GFF2988 at 418.948 kb on - strand, within GFF2988 at 418.948 kb on - strand, within GFF2988 at 419.064 kb on + strand, within GFF2988 at 419.064 kb on + strand, within GFF2988 at 419.065 kb on - strand, within GFF2988 at 419.668 kb on - strand at 419.753 kb on + strand at 419.754 kb on - strand at 419.754 kb on - strand at 419.966 kb on + strand, within GFF2989 at 419.967 kb on - strand, within GFF2989 at 420.029 kb on + strand, within GFF2989 at 420.029 kb on + strand, within GFF2989 at 420.029 kb on + strand, within GFF2989 at 420.030 kb on - strand, within GFF2989 at 420.134 kb on + strand, within GFF2989 at 420.134 kb on + strand, within GFF2989 at 420.201 kb on + strand, within GFF2989 at 420.230 kb on + strand, within GFF2989 at 420.231 kb on - strand, within GFF2989 at 420.589 kb on - strand, within GFF2989 at 420.674 kb on + strand, within GFF2989 at 420.674 kb on + strand, within GFF2989 at 420.707 kb on + strand, within GFF2989 at 420.707 kb on + strand, within GFF2989 at 420.708 kb on - strand, within GFF2989 at 420.708 kb on - strand, within GFF2989 at 420.708 kb on - strand, within GFF2989 at 420.708 kb on - strand, within GFF2989 at 420.905 kb on + strand, within GFF2989 at 420.906 kb on - strand, within GFF2989 at 420.906 kb on - strand, within GFF2989 at 420.906 kb on - strand, within GFF2989 at 421.138 kb on + strand at 421.138 kb on + strand at 421.138 kb on + strand at 421.138 kb on + strand at 421.138 kb on + strand at 421.139 kb on - strand at 421.139 kb on - strand at 421.139 kb on - strand at 421.139 kb on - strand at 421.139 kb on - strand at 421.139 kb on - strand at 421.139 kb on - strand at 421.139 kb on - strand at 421.139 kb on - strand at 421.139 kb on - strand at 421.139 kb on - strand at 421.139 kb on - strand at 421.139 kb on - strand at 421.139 kb on - strand at 421.139 kb on - strand at 421.139 kb on - strand at 421.139 kb on - strand at 421.144 kb on + strand at 421.144 kb on + strand at 421.144 kb on + strand at 421.145 kb on - strand at 421.145 kb on - strand at 421.145 kb on - strand at 421.145 kb on - strand at 421.145 kb on - strand at 421.145 kb on - strand at 421.613 kb on - strand, within GFF2990 at 421.702 kb on + strand, within GFF2990 at 421.703 kb on - strand, within GFF2990 at 421.703 kb on - strand, within GFF2990
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 418,671 + GFF2988 0.26 +0.5 418,698 + GFF2988 0.28 -1.0 418,698 + GFF2988 0.28 -0.5 418,698 + GFF2988 0.28 -0.1 418,698 + GFF2988 0.28 -0.3 418,699 - GFF2988 0.28 -1.3 418,908 + GFF2988 0.44 +0.2 418,908 + GFF2988 0.44 +0.4 418,908 + GFF2988 0.44 +0.5 418,908 + GFF2988 0.44 +0.1 418,908 + GFF2988 0.44 +0.2 418,908 + GFF2988 0.44 +0.0 418,909 - GFF2988 0.44 +0.3 418,947 + GFF2988 0.47 -0.1 418,948 - GFF2988 0.47 +0.3 418,948 - GFF2988 0.47 +0.0 419,064 + GFF2988 0.56 -0.1 419,064 + GFF2988 0.56 -0.3 419,065 - GFF2988 0.56 +0.1 419,668 - -0.3 419,753 + -0.7 419,754 - -1.3 419,754 - -1.3 419,966 + GFF2989 0.23 -1.6 419,967 - GFF2989 0.23 -0.7 420,029 + GFF2989 0.27 +1.8 420,029 + GFF2989 0.27 -0.9 420,029 + GFF2989 0.27 -1.8 420,030 - GFF2989 0.27 -0.8 420,134 + GFF2989 0.35 -0.1 420,134 + GFF2989 0.35 -0.4 420,201 + GFF2989 0.39 -0.1 420,230 + GFF2989 0.41 -1.1 420,231 - GFF2989 0.41 -1.3 420,589 - GFF2989 0.67 -0.5 420,674 + GFF2989 0.73 +0.2 420,674 + GFF2989 0.73 -0.6 420,707 + GFF2989 0.75 +1.1 420,707 + GFF2989 0.75 -0.7 420,708 - GFF2989 0.75 -0.4 420,708 - GFF2989 0.75 -0.1 420,708 - GFF2989 0.75 +0.6 420,708 - GFF2989 0.75 -0.1 420,905 + GFF2989 0.89 -0.1 420,906 - GFF2989 0.89 -0.9 420,906 - GFF2989 0.89 -0.3 420,906 - GFF2989 0.89 -0.3 421,138 + -0.2 421,138 + -0.2 421,138 + +0.4 421,138 + -0.3 421,138 + +0.8 421,139 - -0.4 421,139 - -0.2 421,139 - +0.0 421,139 - +0.0 421,139 - -0.8 421,139 - -2.9 421,139 - -0.4 421,139 - -1.1 421,139 - +1.2 421,139 - -1.2 421,139 - -0.3 421,139 - -2.1 421,139 - -1.5 421,139 - -0.2 421,139 - +0.4 421,139 - -0.2 421,139 - -0.8 421,144 + -0.1 421,144 + -0.6 421,144 + +0.4 421,145 - -0.1 421,145 - -1.5 421,145 - -0.7 421,145 - +0.1 421,145 - -0.1 421,145 - -0.2 421,613 - GFF2990 0.62 +0.0 421,702 + GFF2990 0.73 -1.7 421,703 - GFF2990 0.73 +0.4 421,703 - GFF2990 0.73 -1.2
Or see this region's nucleotide sequence