Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2912 and GFF2913 overlap by 4 nucleotides GFF2913 and GFF2914 overlap by 17 nucleotides GFF2914 and GFF2915 are separated by 56 nucleotides GFF2915 and GFF2916 are separated by 14 nucleotides
GFF2912 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 339,993 to 340,742
GFF2912
GFF2913 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 340,739 to 341,395
GFF2913
GFF2914 - Glutamine ABC transporter, ATP-binding protein GlnQ, at 341,379 to 342,107
GFF2914
GFF2915 - ABC transporter, substrate-binding protein (cluster 3, basic aa/glutamine/opines), at 342,164 to 342,958
GFF2915
GFF2916 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 342,973 to 343,794
GFF2916
Position (kb) 341
342
343 Strain fitness (log2 ratio) -2
-1
0
1
2 at 340.434 kb on + strand, within GFF2912 at 340.434 kb on + strand, within GFF2912 at 340.435 kb on - strand, within GFF2912 at 340.563 kb on + strand, within GFF2912 at 340.563 kb on + strand, within GFF2912 at 340.563 kb on + strand, within GFF2912 at 340.563 kb on + strand, within GFF2912 at 340.563 kb on + strand, within GFF2912 at 340.563 kb on + strand, within GFF2912 at 340.564 kb on - strand, within GFF2912 at 340.564 kb on - strand, within GFF2912 at 340.584 kb on + strand, within GFF2912 at 340.585 kb on - strand, within GFF2912 at 340.635 kb on + strand, within GFF2912 at 340.635 kb on + strand, within GFF2912 at 340.635 kb on + strand, within GFF2912 at 340.635 kb on + strand, within GFF2912 at 340.635 kb on + strand, within GFF2912 at 340.775 kb on + strand at 340.775 kb on + strand at 340.775 kb on + strand at 340.776 kb on - strand at 340.776 kb on - strand at 340.776 kb on - strand at 340.776 kb on - strand at 340.776 kb on - strand at 340.907 kb on + strand, within GFF2913 at 340.908 kb on - strand, within GFF2913 at 340.908 kb on - strand, within GFF2913 at 340.908 kb on - strand, within GFF2913 at 340.908 kb on - strand, within GFF2913 at 340.958 kb on + strand, within GFF2913 at 340.959 kb on - strand, within GFF2913 at 341.027 kb on + strand, within GFF2913 at 341.027 kb on + strand, within GFF2913 at 341.028 kb on - strand, within GFF2913 at 341.028 kb on - strand, within GFF2913 at 341.028 kb on - strand, within GFF2913 at 341.321 kb on + strand, within GFF2913 at 341.322 kb on - strand, within GFF2913 at 341.410 kb on - strand at 341.411 kb on + strand at 341.411 kb on + strand at 341.411 kb on + strand at 341.412 kb on - strand at 341.412 kb on - strand at 341.412 kb on - strand at 341.643 kb on + strand, within GFF2914 at 341.644 kb on - strand, within GFF2914 at 341.644 kb on - strand, within GFF2914 at 341.644 kb on - strand, within GFF2914 at 341.644 kb on - strand, within GFF2914 at 341.644 kb on - strand, within GFF2914 at 341.644 kb on - strand, within GFF2914 at 342.009 kb on + strand, within GFF2914 at 342.009 kb on + strand, within GFF2914 at 342.009 kb on + strand, within GFF2914 at 342.009 kb on + strand, within GFF2914 at 342.009 kb on + strand, within GFF2914 at 342.010 kb on - strand, within GFF2914 at 342.105 kb on + strand at 342.106 kb on - strand at 342.141 kb on - strand at 342.669 kb on - strand, within GFF2915 at 342.908 kb on + strand at 342.908 kb on + strand at 342.909 kb on - strand at 342.917 kb on + strand at 342.917 kb on + strand at 342.918 kb on - strand at 342.918 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 340,434 + GFF2912 0.59 +0.8 340,434 + GFF2912 0.59 +0.1 340,435 - GFF2912 0.59 +0.9 340,563 + GFF2912 0.76 +0.1 340,563 + GFF2912 0.76 +0.2 340,563 + GFF2912 0.76 -0.2 340,563 + GFF2912 0.76 -0.1 340,563 + GFF2912 0.76 +0.3 340,563 + GFF2912 0.76 -1.9 340,564 - GFF2912 0.76 +0.7 340,564 - GFF2912 0.76 -0.8 340,584 + GFF2912 0.79 -1.8 340,585 - GFF2912 0.79 -0.6 340,635 + GFF2912 0.86 -0.9 340,635 + GFF2912 0.86 -0.4 340,635 + GFF2912 0.86 -0.9 340,635 + GFF2912 0.86 -1.3 340,635 + GFF2912 0.86 +0.2 340,775 + -1.3 340,775 + -0.2 340,775 + -0.5 340,776 - +1.0 340,776 - -0.5 340,776 - -1.0 340,776 - -0.1 340,776 - +2.5 340,907 + GFF2913 0.26 +1.0 340,908 - GFF2913 0.26 -1.3 340,908 - GFF2913 0.26 -2.1 340,908 - GFF2913 0.26 -0.1 340,908 - GFF2913 0.26 -0.1 340,958 + GFF2913 0.33 -1.5 340,959 - GFF2913 0.33 +0.7 341,027 + GFF2913 0.44 +1.9 341,027 + GFF2913 0.44 -0.8 341,028 - GFF2913 0.44 -1.9 341,028 - GFF2913 0.44 -0.1 341,028 - GFF2913 0.44 -0.3 341,321 + GFF2913 0.89 -1.2 341,322 - GFF2913 0.89 +0.2 341,410 - -1.1 341,411 + +0.5 341,411 + -1.3 341,411 + +0.5 341,412 - +0.8 341,412 - -0.1 341,412 - -0.7 341,643 + GFF2914 0.36 -1.1 341,644 - GFF2914 0.36 -0.5 341,644 - GFF2914 0.36 -0.4 341,644 - GFF2914 0.36 -2.7 341,644 - GFF2914 0.36 -0.5 341,644 - GFF2914 0.36 -1.5 341,644 - GFF2914 0.36 -0.5 342,009 + GFF2914 0.86 +0.2 342,009 + GFF2914 0.86 +0.6 342,009 + GFF2914 0.86 -2.5 342,009 + GFF2914 0.86 +1.2 342,009 + GFF2914 0.86 -0.7 342,010 - GFF2914 0.87 +2.4 342,105 + +0.1 342,106 - +0.7 342,141 - -0.0 342,669 - GFF2915 0.64 +1.5 342,908 + +0.1 342,908 + +0.7 342,909 - +0.1 342,917 + +0.7 342,917 + -1.8 342,918 - -0.9 342,918 - +0.2
Or see this region's nucleotide sequence