Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2655 and GFF2656 are separated by 155 nucleotides GFF2656 and GFF2657 overlap by 4 nucleotides
GFF2655 - Efflux ABC transporter for glutathione/L-cysteine, essential for assembly of bd-type respiratory oxidases => CydD subunit, at 75,489 to 77,177
GFF2655
GFF2656 - Acyltransferase 3, at 77,333 to 78,508
GFF2656
GFF2657 - putative oxidoreductase, nitronate monooxygenase family, at 78,505 to 79,467
GFF2657
Position (kb)
77
78
79 Strain fitness (log2 ratio)
-2
-1
0
1 at 76.402 kb on + strand, within GFF2655 at 76.402 kb on + strand, within GFF2655 at 76.402 kb on + strand, within GFF2655 at 76.402 kb on + strand, within GFF2655 at 76.402 kb on + strand, within GFF2655 at 76.402 kb on + strand, within GFF2655 at 76.402 kb on + strand, within GFF2655 at 76.403 kb on - strand, within GFF2655 at 76.403 kb on - strand, within GFF2655 at 76.403 kb on - strand, within GFF2655 at 76.403 kb on - strand, within GFF2655 at 76.403 kb on - strand, within GFF2655 at 76.403 kb on - strand, within GFF2655 at 76.403 kb on - strand, within GFF2655 at 76.403 kb on - strand, within GFF2655 at 76.724 kb on - strand, within GFF2655 at 76.724 kb on - strand, within GFF2655 at 77.348 kb on + strand at 77.349 kb on - strand at 77.349 kb on - strand at 77.425 kb on - strand at 77.565 kb on + strand, within GFF2656 at 77.805 kb on + strand, within GFF2656 at 77.805 kb on + strand, within GFF2656 at 77.806 kb on - strand, within GFF2656 at 77.806 kb on - strand, within GFF2656 at 77.806 kb on - strand, within GFF2656 at 77.821 kb on - strand, within GFF2656 at 78.015 kb on + strand, within GFF2656 at 78.016 kb on - strand, within GFF2656 at 78.042 kb on + strand, within GFF2656 at 78.043 kb on - strand, within GFF2656 at 78.094 kb on + strand, within GFF2656 at 78.094 kb on + strand, within GFF2656 at 78.094 kb on + strand, within GFF2656 at 78.094 kb on + strand, within GFF2656 at 78.094 kb on + strand, within GFF2656 at 78.094 kb on + strand, within GFF2656 at 78.095 kb on - strand, within GFF2656 at 78.095 kb on - strand, within GFF2656 at 78.095 kb on - strand, within GFF2656 at 78.095 kb on - strand, within GFF2656 at 78.095 kb on - strand, within GFF2656 at 78.354 kb on + strand, within GFF2656 at 78.379 kb on - strand, within GFF2656 at 78.536 kb on + strand at 78.537 kb on - strand at 78.734 kb on + strand, within GFF2657 at 78.735 kb on - strand, within GFF2657 at 78.809 kb on + strand, within GFF2657 at 78.809 kb on + strand, within GFF2657 at 78.809 kb on + strand, within GFF2657 at 78.809 kb on + strand, within GFF2657 at 78.810 kb on - strand, within GFF2657 at 79.101 kb on - strand, within GFF2657 at 79.101 kb on - strand, within GFF2657 at 79.101 kb on - strand, within GFF2657 at 79.101 kb on - strand, within GFF2657 at 79.166 kb on + strand, within GFF2657 at 79.166 kb on + strand, within GFF2657 at 79.166 kb on + strand, within GFF2657 at 79.166 kb on + strand, within GFF2657 at 79.167 kb on - strand, within GFF2657 at 79.271 kb on + strand, within GFF2657 at 79.271 kb on + strand, within GFF2657 at 79.496 kb on + strand at 79.496 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 76,402 + GFF2655 0.54 -0.7 76,402 + GFF2655 0.54 -1.0 76,402 + GFF2655 0.54 -1.1 76,402 + GFF2655 0.54 -1.2 76,402 + GFF2655 0.54 +0.6 76,402 + GFF2655 0.54 -0.6 76,402 + GFF2655 0.54 -0.1 76,403 - GFF2655 0.54 -0.3 76,403 - GFF2655 0.54 -0.5 76,403 - GFF2655 0.54 -0.1 76,403 - GFF2655 0.54 +0.2 76,403 - GFF2655 0.54 -0.8 76,403 - GFF2655 0.54 +0.5 76,403 - GFF2655 0.54 -0.1 76,403 - GFF2655 0.54 +0.2 76,724 - GFF2655 0.73 +0.4 76,724 - GFF2655 0.73 -0.4 77,348 + -0.3 77,349 - +1.5 77,349 - -0.7 77,425 - +0.4 77,565 + GFF2656 0.20 -0.1 77,805 + GFF2656 0.40 +0.3 77,805 + GFF2656 0.40 +0.3 77,806 - GFF2656 0.40 +0.2 77,806 - GFF2656 0.40 -0.7 77,806 - GFF2656 0.40 -0.2 77,821 - GFF2656 0.41 +0.0 78,015 + GFF2656 0.58 -0.2 78,016 - GFF2656 0.58 +0.1 78,042 + GFF2656 0.60 +0.2 78,043 - GFF2656 0.60 +0.9 78,094 + GFF2656 0.65 -0.6 78,094 + GFF2656 0.65 -0.1 78,094 + GFF2656 0.65 -0.2 78,094 + GFF2656 0.65 -0.1 78,094 + GFF2656 0.65 +0.4 78,094 + GFF2656 0.65 +0.4 78,095 - GFF2656 0.65 +1.3 78,095 - GFF2656 0.65 -1.0 78,095 - GFF2656 0.65 +0.1 78,095 - GFF2656 0.65 +0.2 78,095 - GFF2656 0.65 -0.3 78,354 + GFF2656 0.87 +0.2 78,379 - GFF2656 0.89 -0.3 78,536 + -0.7 78,537 - +0.0 78,734 + GFF2657 0.24 -0.5 78,735 - GFF2657 0.24 -1.3 78,809 + GFF2657 0.32 +0.1 78,809 + GFF2657 0.32 -2.2 78,809 + GFF2657 0.32 -1.0 78,809 + GFF2657 0.32 +0.2 78,810 - GFF2657 0.32 -0.5 79,101 - GFF2657 0.62 -0.1 79,101 - GFF2657 0.62 -1.3 79,101 - GFF2657 0.62 -0.3 79,101 - GFF2657 0.62 +0.3 79,166 + GFF2657 0.69 -0.1 79,166 + GFF2657 0.69 +0.7 79,166 + GFF2657 0.69 -0.8 79,166 + GFF2657 0.69 +0.1 79,167 - GFF2657 0.69 -0.2 79,271 + GFF2657 0.80 -0.5 79,271 + GFF2657 0.80 -0.2 79,496 + +0.2 79,496 + -0.3
Or see this region's nucleotide sequence