Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2652 and GFF2653 overlap by 4 nucleotides GFF2653 and GFF2654 are separated by 47 nucleotides
GFF2652 - Cytochrome d ubiquinol oxidase subunit II (EC 1.10.3.-), at 70,965 to 72,119
GFF2652
GFF2653 - Cytochrome d ubiquinol oxidase subunit I (EC 1.10.3.-), at 72,116 to 73,729
GFF2653
GFF2654 - Efflux ABC transporter for glutathione/L-cysteine, essential for assembly of bd-type respiratory oxidases => CydC subunit, at 73,777 to 75,492
GFF2654
Position (kb)
72
73
74 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 71.329 kb on + strand, within GFF2652 at 71.329 kb on + strand, within GFF2652 at 71.461 kb on + strand, within GFF2652 at 71.461 kb on + strand, within GFF2652 at 71.462 kb on - strand, within GFF2652 at 71.462 kb on - strand, within GFF2652 at 71.462 kb on - strand, within GFF2652 at 71.462 kb on - strand, within GFF2652 at 71.462 kb on - strand, within GFF2652 at 71.497 kb on + strand, within GFF2652 at 71.497 kb on + strand, within GFF2652 at 71.497 kb on + strand, within GFF2652 at 71.636 kb on - strand, within GFF2652 at 71.845 kb on + strand, within GFF2652 at 71.846 kb on - strand, within GFF2652 at 71.869 kb on + strand, within GFF2652 at 71.870 kb on - strand, within GFF2652 at 72.094 kb on + strand at 72.094 kb on + strand at 72.095 kb on - strand at 72.270 kb on + strand at 72.270 kb on + strand at 72.270 kb on + strand at 72.271 kb on - strand at 72.636 kb on + strand, within GFF2653 at 72.636 kb on + strand, within GFF2653 at 72.637 kb on - strand, within GFF2653 at 72.637 kb on - strand, within GFF2653 at 72.637 kb on - strand, within GFF2653 at 72.754 kb on - strand, within GFF2653 at 72.754 kb on - strand, within GFF2653 at 72.876 kb on + strand, within GFF2653 at 72.876 kb on + strand, within GFF2653 at 72.876 kb on + strand, within GFF2653 at 73.111 kb on - strand, within GFF2653 at 73.152 kb on + strand, within GFF2653 at 73.152 kb on + strand, within GFF2653 at 73.152 kb on + strand, within GFF2653 at 73.153 kb on - strand, within GFF2653 at 73.153 kb on - strand, within GFF2653 at 73.457 kb on - strand, within GFF2653 at 73.477 kb on - strand, within GFF2653 at 73.479 kb on + strand, within GFF2653 at 73.480 kb on - strand, within GFF2653 at 73.608 kb on + strand at 73.609 kb on - strand at 73.674 kb on + strand at 73.675 kb on - strand at 73.675 kb on - strand at 73.745 kb on + strand at 73.745 kb on + strand at 73.746 kb on - strand at 73.746 kb on - strand at 73.746 kb on - strand at 74.583 kb on + strand, within GFF2654
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 71,329 + GFF2652 0.32 +1.3 71,329 + GFF2652 0.32 -0.0 71,461 + GFF2652 0.43 +0.4 71,461 + GFF2652 0.43 -0.5 71,462 - GFF2652 0.43 +1.2 71,462 - GFF2652 0.43 -0.5 71,462 - GFF2652 0.43 +0.0 71,462 - GFF2652 0.43 -0.5 71,462 - GFF2652 0.43 -0.5 71,497 + GFF2652 0.46 +0.0 71,497 + GFF2652 0.46 -0.1 71,497 + GFF2652 0.46 +0.5 71,636 - GFF2652 0.58 -1.7 71,845 + GFF2652 0.76 +0.7 71,846 - GFF2652 0.76 +0.7 71,869 + GFF2652 0.78 -1.8 71,870 - GFF2652 0.78 +2.5 72,094 + +0.1 72,094 + -0.3 72,095 - +0.5 72,270 + +0.5 72,270 + -0.8 72,270 + -0.3 72,271 - +0.1 72,636 + GFF2653 0.32 +1.3 72,636 + GFF2653 0.32 -0.6 72,637 - GFF2653 0.32 -0.3 72,637 - GFF2653 0.32 -0.5 72,637 - GFF2653 0.32 +0.4 72,754 - GFF2653 0.40 -1.3 72,754 - GFF2653 0.40 +1.0 72,876 + GFF2653 0.47 +0.0 72,876 + GFF2653 0.47 -0.7 72,876 + GFF2653 0.47 -1.1 73,111 - GFF2653 0.62 -0.9 73,152 + GFF2653 0.64 +0.3 73,152 + GFF2653 0.64 -1.1 73,152 + GFF2653 0.64 -2.1 73,153 - GFF2653 0.64 -2.1 73,153 - GFF2653 0.64 -0.2 73,457 - GFF2653 0.83 -0.3 73,477 - GFF2653 0.84 +0.6 73,479 + GFF2653 0.84 -0.8 73,480 - GFF2653 0.85 -0.8 73,608 + -0.3 73,609 - -1.5 73,674 + -0.0 73,675 - -0.8 73,675 - -1.3 73,745 + -1.2 73,745 + -0.6 73,746 - +0.1 73,746 - -0.8 73,746 - -2.6 74,583 + GFF2654 0.47 -0.6
Or see this region's nucleotide sequence