Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2299 and GFF2300 are separated by 25 nucleotides GFF2300 and GFF2301 overlap by 1 nucleotides GFF2301 and GFF2302 are separated by 52 nucleotides GFF2302 and GFF2303 are separated by 77 nucleotides GFF2303 and GFF2304 are separated by 36 nucleotides
GFF2299 - hypothetical protein, at 353,428 to 353,544
GFF2299
GFF2300 - Cytochrome oxidase biogenesis protein Cox11-CtaG, copper delivery to Cox1, at 353,570 to 354,196
GFF2300
GFF2301 - no description, at 354,196 to 354,417
GFF2301
GFF2302 - Cytochrome c oxidase polypeptide III (EC 1.9.3.1), at 354,470 to 355,351
GFF2302
GFF2303 - Uncharacterized DUF2909 protein BB4826, at 355,429 to 355,650
GFF2303
GFF2304 - Cytochrome oxidase biogenesis protein Surf1, facilitates heme A insertion, at 355,687 to 356,541
GFF2304
Position (kb) 354
355
356 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 353.587 kb on + strand at 353.587 kb on + strand at 353.587 kb on + strand at 353.587 kb on + strand at 353.588 kb on - strand at 353.657 kb on + strand, within GFF2300 at 353.658 kb on - strand, within GFF2300 at 353.676 kb on - strand, within GFF2300 at 353.676 kb on - strand, within GFF2300 at 353.676 kb on - strand, within GFF2300 at 353.905 kb on - strand, within GFF2300 at 353.906 kb on + strand, within GFF2300 at 353.906 kb on + strand, within GFF2300 at 353.906 kb on + strand, within GFF2300 at 353.906 kb on + strand, within GFF2300 at 353.906 kb on + strand, within GFF2300 at 353.906 kb on + strand, within GFF2300 at 353.906 kb on + strand, within GFF2300 at 353.906 kb on + strand, within GFF2300 at 353.906 kb on + strand, within GFF2300 at 353.906 kb on + strand, within GFF2300 at 353.906 kb on + strand, within GFF2300 at 353.906 kb on + strand, within GFF2300 at 353.907 kb on - strand, within GFF2300 at 353.907 kb on - strand, within GFF2300 at 353.907 kb on - strand, within GFF2300 at 353.907 kb on - strand, within GFF2300 at 353.907 kb on - strand, within GFF2300 at 353.907 kb on - strand, within GFF2300 at 353.960 kb on + strand, within GFF2300 at 353.960 kb on + strand, within GFF2300 at 353.961 kb on - strand, within GFF2300 at 353.961 kb on - strand, within GFF2300 at 353.984 kb on + strand, within GFF2300 at 353.984 kb on + strand, within GFF2300 at 353.984 kb on + strand, within GFF2300 at 354.020 kb on + strand, within GFF2300 at 354.020 kb on + strand, within GFF2300 at 354.020 kb on + strand, within GFF2300 at 354.021 kb on - strand, within GFF2300 at 354.113 kb on + strand, within GFF2300 at 354.113 kb on + strand, within GFF2300 at 354.113 kb on + strand, within GFF2300 at 354.113 kb on + strand, within GFF2300 at 354.113 kb on + strand, within GFF2300 at 354.113 kb on + strand, within GFF2300 at 354.114 kb on - strand, within GFF2300 at 354.114 kb on - strand, within GFF2300 at 354.114 kb on - strand, within GFF2300 at 354.114 kb on - strand, within GFF2300 at 354.301 kb on + strand, within GFF2301 at 354.301 kb on + strand, within GFF2301 at 354.469 kb on + strand at 354.469 kb on + strand at 354.469 kb on + strand at 354.469 kb on + strand at 354.470 kb on - strand at 354.470 kb on - strand at 354.470 kb on - strand at 354.470 kb on - strand at 354.470 kb on - strand at 354.470 kb on - strand at 354.470 kb on - strand at 354.500 kb on + strand at 354.500 kb on + strand at 354.500 kb on + strand at 354.503 kb on + strand at 354.503 kb on + strand at 354.503 kb on + strand at 354.503 kb on + strand at 354.503 kb on + strand at 354.503 kb on + strand at 354.504 kb on - strand at 354.504 kb on - strand at 354.504 kb on - strand at 354.504 kb on - strand at 354.504 kb on - strand at 354.504 kb on - strand at 354.504 kb on - strand at 354.696 kb on - strand, within GFF2302 at 354.742 kb on + strand, within GFF2302 at 354.742 kb on + strand, within GFF2302 at 354.742 kb on + strand, within GFF2302 at 354.742 kb on + strand, within GFF2302 at 354.742 kb on + strand, within GFF2302 at 354.743 kb on - strand, within GFF2302 at 354.743 kb on - strand, within GFF2302 at 355.094 kb on + strand, within GFF2302 at 355.094 kb on + strand, within GFF2302 at 355.094 kb on + strand, within GFF2302 at 355.095 kb on - strand, within GFF2302 at 355.100 kb on + strand, within GFF2302 at 355.100 kb on + strand, within GFF2302 at 355.100 kb on + strand, within GFF2302 at 355.100 kb on + strand, within GFF2302 at 355.100 kb on + strand, within GFF2302 at 355.101 kb on - strand, within GFF2302 at 355.101 kb on - strand, within GFF2302 at 355.101 kb on - strand, within GFF2302 at 355.101 kb on - strand, within GFF2302 at 355.112 kb on + strand, within GFF2302 at 355.112 kb on + strand, within GFF2302 at 355.112 kb on + strand, within GFF2302 at 355.113 kb on - strand, within GFF2302 at 355.113 kb on - strand, within GFF2302 at 355.113 kb on - strand, within GFF2302 at 355.113 kb on - strand, within GFF2302 at 355.113 kb on - strand, within GFF2302 at 355.148 kb on + strand, within GFF2302 at 355.148 kb on + strand, within GFF2302 at 355.148 kb on + strand, within GFF2302 at 355.148 kb on + strand, within GFF2302 at 355.149 kb on - strand, within GFF2302 at 355.149 kb on - strand, within GFF2302 at 355.149 kb on - strand, within GFF2302 at 355.149 kb on - strand, within GFF2302 at 355.149 kb on - strand, within GFF2302 at 355.292 kb on + strand at 355.292 kb on + strand at 355.328 kb on + strand at 355.328 kb on + strand at 355.328 kb on + strand at 355.328 kb on + strand at 355.328 kb on + strand at 355.328 kb on + strand at 355.328 kb on + strand at 355.328 kb on + strand at 355.329 kb on - strand at 355.329 kb on - strand at 355.329 kb on - strand at 355.329 kb on - strand at 355.329 kb on - strand at 355.329 kb on - strand at 355.329 kb on - strand at 355.340 kb on + strand at 355.341 kb on - strand at 355.430 kb on + strand at 355.430 kb on + strand at 355.430 kb on + strand at 355.430 kb on + strand at 355.430 kb on + strand at 355.430 kb on + strand at 355.430 kb on + strand at 355.430 kb on + strand at 355.430 kb on + strand at 355.430 kb on + strand at 355.430 kb on + strand at 355.430 kb on + strand at 355.430 kb on + strand at 355.431 kb on - strand at 355.431 kb on - strand at 355.431 kb on - strand at 355.431 kb on - strand at 355.431 kb on - strand at 355.431 kb on - strand at 355.431 kb on - strand at 355.431 kb on - strand at 355.431 kb on - strand at 355.431 kb on - strand at 355.431 kb on - strand at 355.463 kb on + strand, within GFF2303 at 355.463 kb on + strand, within GFF2303 at 355.463 kb on + strand, within GFF2303 at 355.463 kb on + strand, within GFF2303 at 355.463 kb on + strand, within GFF2303 at 355.463 kb on + strand, within GFF2303 at 355.463 kb on + strand, within GFF2303 at 355.464 kb on - strand, within GFF2303 at 355.464 kb on - strand, within GFF2303 at 355.464 kb on - strand, within GFF2303 at 355.464 kb on - strand, within GFF2303 at 355.464 kb on - strand, within GFF2303 at 355.643 kb on + strand at 355.644 kb on - strand at 355.644 kb on - strand at 355.644 kb on - strand at 355.660 kb on + strand at 355.660 kb on + strand at 355.661 kb on - strand at 355.661 kb on - strand at 355.661 kb on - strand at 355.705 kb on + strand at 355.705 kb on + strand at 355.705 kb on + strand at 355.706 kb on - strand at 355.706 kb on - strand at 355.907 kb on - strand, within GFF2304 at 355.963 kb on + strand, within GFF2304 at 355.963 kb on + strand, within GFF2304 at 355.963 kb on + strand, within GFF2304 at 355.964 kb on - strand, within GFF2304 at 356.002 kb on + strand, within GFF2304 at 356.002 kb on + strand, within GFF2304 at 356.002 kb on + strand, within GFF2304 at 356.002 kb on + strand, within GFF2304 at 356.002 kb on + strand, within GFF2304 at 356.002 kb on + strand, within GFF2304 at 356.003 kb on - strand, within GFF2304 at 356.003 kb on - strand, within GFF2304 at 356.003 kb on - strand, within GFF2304 at 356.003 kb on - strand, within GFF2304 at 356.003 kb on - strand, within GFF2304 at 356.003 kb on - strand, within GFF2304 at 356.269 kb on + strand, within GFF2304 at 356.270 kb on - strand, within GFF2304 at 356.335 kb on - strand, within GFF2304
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 353,587 + -0.9 353,587 + -0.4 353,587 + -1.8 353,587 + -0.5 353,588 - -1.9 353,657 + GFF2300 0.14 -1.8 353,658 - GFF2300 0.14 +0.1 353,676 - GFF2300 0.17 -1.1 353,676 - GFF2300 0.17 -1.7 353,676 - GFF2300 0.17 -1.0 353,905 - GFF2300 0.53 -3.0 353,906 + GFF2300 0.54 -0.2 353,906 + GFF2300 0.54 +0.1 353,906 + GFF2300 0.54 -1.1 353,906 + GFF2300 0.54 -1.9 353,906 + GFF2300 0.54 -1.9 353,906 + GFF2300 0.54 -2.2 353,906 + GFF2300 0.54 -1.1 353,906 + GFF2300 0.54 -0.5 353,906 + GFF2300 0.54 +1.7 353,906 + GFF2300 0.54 -0.8 353,906 + GFF2300 0.54 -2.5 353,906 + GFF2300 0.54 -0.9 353,907 - GFF2300 0.54 -1.4 353,907 - GFF2300 0.54 -1.0 353,907 - GFF2300 0.54 -2.4 353,907 - GFF2300 0.54 +0.1 353,907 - GFF2300 0.54 -1.6 353,907 - GFF2300 0.54 -2.2 353,960 + GFF2300 0.62 +0.2 353,960 + GFF2300 0.62 +0.5 353,961 - GFF2300 0.62 -0.9 353,961 - GFF2300 0.62 -1.4 353,984 + GFF2300 0.66 +1.4 353,984 + GFF2300 0.66 -1.3 353,984 + GFF2300 0.66 -0.6 354,020 + GFF2300 0.72 +0.1 354,020 + GFF2300 0.72 -1.0 354,020 + GFF2300 0.72 -3.4 354,021 - GFF2300 0.72 -1.2 354,113 + GFF2300 0.87 -0.3 354,113 + GFF2300 0.87 -0.6 354,113 + GFF2300 0.87 +0.6 354,113 + GFF2300 0.87 -0.2 354,113 + GFF2300 0.87 -0.9 354,113 + GFF2300 0.87 -1.1 354,114 - GFF2300 0.87 -1.9 354,114 - GFF2300 0.87 -1.2 354,114 - GFF2300 0.87 -1.5 354,114 - GFF2300 0.87 -0.5 354,301 + GFF2301 0.47 -0.2 354,301 + GFF2301 0.47 -0.7 354,469 + -1.2 354,469 + -1.4 354,469 + -0.8 354,469 + +0.0 354,470 - -0.3 354,470 - +0.4 354,470 - -1.3 354,470 - -0.4 354,470 - -2.2 354,470 - -1.0 354,470 - -0.8 354,500 + -1.1 354,500 + -1.1 354,500 + -0.3 354,503 + +0.4 354,503 + -1.4 354,503 + -0.6 354,503 + -1.6 354,503 + -0.9 354,503 + -0.3 354,504 - -1.1 354,504 - -2.4 354,504 - -0.1 354,504 - -1.3 354,504 - -2.3 354,504 - -1.3 354,504 - -0.1 354,696 - GFF2302 0.26 -0.6 354,742 + GFF2302 0.31 -0.3 354,742 + GFF2302 0.31 -1.5 354,742 + GFF2302 0.31 -1.4 354,742 + GFF2302 0.31 -0.5 354,742 + GFF2302 0.31 -0.6 354,743 - GFF2302 0.31 -0.9 354,743 - GFF2302 0.31 +0.7 355,094 + GFF2302 0.71 -0.3 355,094 + GFF2302 0.71 -1.1 355,094 + GFF2302 0.71 -2.0 355,095 - GFF2302 0.71 -1.1 355,100 + GFF2302 0.71 -1.1 355,100 + GFF2302 0.71 -0.3 355,100 + GFF2302 0.71 -0.6 355,100 + GFF2302 0.71 -2.8 355,100 + GFF2302 0.71 -1.0 355,101 - GFF2302 0.72 -1.8 355,101 - GFF2302 0.72 -1.7 355,101 - GFF2302 0.72 +1.1 355,101 - GFF2302 0.72 -1.5 355,112 + GFF2302 0.73 -1.5 355,112 + GFF2302 0.73 +0.0 355,112 + GFF2302 0.73 -0.9 355,113 - GFF2302 0.73 -1.3 355,113 - GFF2302 0.73 -1.2 355,113 - GFF2302 0.73 -0.0 355,113 - GFF2302 0.73 -1.3 355,113 - GFF2302 0.73 -1.9 355,148 + GFF2302 0.77 -0.5 355,148 + GFF2302 0.77 +0.1 355,148 + GFF2302 0.77 -0.8 355,148 + GFF2302 0.77 -0.1 355,149 - GFF2302 0.77 -0.9 355,149 - GFF2302 0.77 -0.2 355,149 - GFF2302 0.77 +0.2 355,149 - GFF2302 0.77 -0.5 355,149 - GFF2302 0.77 -0.4 355,292 + -2.6 355,292 + -0.3 355,328 + -1.1 355,328 + -2.6 355,328 + -1.1 355,328 + -1.4 355,328 + -0.2 355,328 + -0.2 355,328 + -0.3 355,328 + -0.2 355,329 - -0.5 355,329 - -0.9 355,329 - -0.7 355,329 - -1.2 355,329 - -3.1 355,329 - -0.4 355,329 - -0.6 355,340 + -0.9 355,341 - -0.7 355,430 + -0.6 355,430 + -0.6 355,430 + -0.8 355,430 + -1.5 355,430 + +0.6 355,430 + +0.2 355,430 + -0.0 355,430 + +0.4 355,430 + -0.9 355,430 + -0.8 355,430 + +0.8 355,430 + +0.4 355,430 + -0.5 355,431 - +0.3 355,431 - -0.4 355,431 - +0.0 355,431 - -0.6 355,431 - -1.3 355,431 - -0.4 355,431 - +0.3 355,431 - -0.6 355,431 - -1.1 355,431 - +1.1 355,431 - +0.3 355,463 + GFF2303 0.15 +0.3 355,463 + GFF2303 0.15 -0.3 355,463 + GFF2303 0.15 -1.4 355,463 + GFF2303 0.15 -0.7 355,463 + GFF2303 0.15 -0.6 355,463 + GFF2303 0.15 -0.4 355,463 + GFF2303 0.15 +0.7 355,464 - GFF2303 0.16 -1.0 355,464 - GFF2303 0.16 -0.6 355,464 - GFF2303 0.16 -1.0 355,464 - GFF2303 0.16 +0.5 355,464 - GFF2303 0.16 +1.1 355,643 + -1.3 355,644 - -2.7 355,644 - -1.0 355,644 - -0.9 355,660 + -0.1 355,660 + -0.5 355,661 - -0.9 355,661 - -0.5 355,661 - -0.9 355,705 + -0.4 355,705 + -1.6 355,705 + -0.4 355,706 - -0.4 355,706 - -1.4 355,907 - GFF2304 0.26 -0.7 355,963 + GFF2304 0.32 -1.5 355,963 + GFF2304 0.32 -1.1 355,963 + GFF2304 0.32 -0.2 355,964 - GFF2304 0.32 -0.7 356,002 + GFF2304 0.37 -1.6 356,002 + GFF2304 0.37 -0.2 356,002 + GFF2304 0.37 -2.3 356,002 + GFF2304 0.37 -0.9 356,002 + GFF2304 0.37 -1.1 356,002 + GFF2304 0.37 -0.2 356,003 - GFF2304 0.37 -0.3 356,003 - GFF2304 0.37 -1.6 356,003 - GFF2304 0.37 -1.9 356,003 - GFF2304 0.37 -0.9 356,003 - GFF2304 0.37 -0.9 356,003 - GFF2304 0.37 -1.5 356,269 + GFF2304 0.68 -1.5 356,270 - GFF2304 0.68 -0.7 356,335 - GFF2304 0.76 -1.7
Or see this region's nucleotide sequence