Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2252 and GFF2253 are separated by 4 nucleotides GFF2253 and GFF2254 are separated by 58 nucleotides GFF2254 and GFF2255 are separated by 8 nucleotides
GFF2252 - RNA-2',3'-PO4:RNA-5'-OH ligase, at 308,221 to 309,456
GFF2252
GFF2253 - no description, at 309,461 to 309,748
GFF2253
GFF2254 - HYPOTHETICAL/UNKNOWN PROTEIN, at 309,807 to 310,448
GFF2254
GFF2255 - Ribonuclease Z (EC 3.1.26.11), at 310,457 to 311,413
GFF2255
Position (kb)
309
310 Strain fitness (log2 ratio)
-2
-1
0
1 at 308.936 kb on - strand, within GFF2252 at 309.076 kb on + strand, within GFF2252 at 309.076 kb on + strand, within GFF2252 at 309.076 kb on + strand, within GFF2252 at 309.076 kb on + strand, within GFF2252 at 309.077 kb on - strand, within GFF2252 at 309.265 kb on - strand, within GFF2252 at 309.422 kb on - strand at 309.422 kb on - strand at 309.422 kb on - strand at 309.578 kb on + strand, within GFF2253 at 309.578 kb on + strand, within GFF2253 at 309.579 kb on - strand, within GFF2253 at 309.745 kb on - strand at 309.745 kb on - strand at 309.747 kb on - strand at 309.747 kb on - strand at 309.747 kb on - strand at 309.747 kb on - strand at 309.747 kb on - strand at 309.747 kb on - strand at 309.780 kb on + strand at 309.781 kb on - strand at 309.781 kb on - strand at 309.788 kb on + strand at 309.969 kb on + strand, within GFF2254 at 309.970 kb on - strand, within GFF2254 at 309.970 kb on - strand, within GFF2254 at 309.982 kb on - strand, within GFF2254 at 310.003 kb on - strand, within GFF2254 at 310.003 kb on - strand, within GFF2254 at 310.003 kb on - strand, within GFF2254 at 310.077 kb on + strand, within GFF2254 at 310.078 kb on - strand, within GFF2254 at 310.078 kb on - strand, within GFF2254 at 310.078 kb on - strand, within GFF2254 at 310.078 kb on - strand, within GFF2254 at 310.097 kb on + strand, within GFF2254 at 310.098 kb on - strand, within GFF2254 at 310.387 kb on - strand at 310.664 kb on + strand, within GFF2255
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 308,936 - GFF2252 0.58 -0.8 309,076 + GFF2252 0.69 -0.6 309,076 + GFF2252 0.69 -0.6 309,076 + GFF2252 0.69 +0.6 309,076 + GFF2252 0.69 -0.3 309,077 - GFF2252 0.69 -0.9 309,265 - GFF2252 0.84 +0.6 309,422 - +0.7 309,422 - -0.1 309,422 - -0.1 309,578 + GFF2253 0.41 -0.5 309,578 + GFF2253 0.41 -0.2 309,579 - GFF2253 0.41 -1.8 309,745 - +0.4 309,745 - +0.7 309,747 - -0.8 309,747 - +0.1 309,747 - -0.9 309,747 - -0.9 309,747 - +0.1 309,747 - -0.3 309,780 + -1.3 309,781 - -0.7 309,781 - -0.8 309,788 + -0.7 309,969 + GFF2254 0.25 +0.1 309,970 - GFF2254 0.25 -0.2 309,970 - GFF2254 0.25 +0.4 309,982 - GFF2254 0.27 -0.1 310,003 - GFF2254 0.31 +0.2 310,003 - GFF2254 0.31 +0.1 310,003 - GFF2254 0.31 +0.1 310,077 + GFF2254 0.42 -0.6 310,078 - GFF2254 0.42 -0.6 310,078 - GFF2254 0.42 +0.3 310,078 - GFF2254 0.42 +0.3 310,078 - GFF2254 0.42 -1.1 310,097 + GFF2254 0.45 -0.8 310,098 - GFF2254 0.45 -0.9 310,387 - -0.5 310,664 + GFF2255 0.22 +0.4
Or see this region's nucleotide sequence