Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2157 and GFF2158 are separated by 8 nucleotides GFF2158 and GFF2159 overlap by 4 nucleotides GFF2159 and GFF2160 overlap by 1 nucleotides GFF2160 and GFF2161 overlap by 4 nucleotides
GFF2157 - UPF0301 protein YqgE, at 210,912 to 211,511
GFF2157
GFF2158 - Putative pre-16S rRNA nuclease YqgF, at 211,520 to 211,969
GFF2158
GFF2159 - Pyrimidine operon regulatory protein PyrR, at 211,966 to 212,463
GFF2159
GFF2160 - Aspartate carbamoyltransferase (EC 2.1.3.2), at 212,463 to 213,425
GFF2160
GFF2161 - Dihydroorotase (EC 3.5.2.3), at 213,422 to 214,711
GFF2161
Position (kb) 212
213
214 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 211.996 kb on + strand at 211.996 kb on + strand at 211.996 kb on + strand at 211.997 kb on - strand at 211.997 kb on - strand at 212.018 kb on + strand, within GFF2159 at 212.019 kb on - strand, within GFF2159 at 212.245 kb on + strand, within GFF2159 at 212.245 kb on + strand, within GFF2159 at 212.245 kb on + strand, within GFF2159 at 212.245 kb on + strand, within GFF2159 at 212.246 kb on - strand, within GFF2159 at 212.246 kb on - strand, within GFF2159 at 212.246 kb on - strand, within GFF2159 at 212.246 kb on - strand, within GFF2159 at 212.246 kb on - strand, within GFF2159 at 212.461 kb on + strand at 212.461 kb on + strand at 212.461 kb on + strand at 212.461 kb on + strand at 212.461 kb on + strand at 212.461 kb on + strand at 212.461 kb on + strand at 212.461 kb on + strand at 212.461 kb on + strand at 212.461 kb on + strand at 212.462 kb on - strand at 212.462 kb on - strand at 212.462 kb on - strand at 212.462 kb on - strand at 212.462 kb on - strand at 212.462 kb on - strand at 212.462 kb on - strand at 212.462 kb on - strand at 212.462 kb on - strand at 212.462 kb on - strand at 212.462 kb on - strand at 212.462 kb on - strand at 212.469 kb on + strand at 212.469 kb on + strand at 212.469 kb on + strand at 212.469 kb on + strand at 212.469 kb on + strand at 212.470 kb on - strand at 212.470 kb on - strand at 212.470 kb on - strand at 212.470 kb on - strand at 212.838 kb on + strand, within GFF2160 at 212.838 kb on + strand, within GFF2160 at 212.839 kb on - strand, within GFF2160 at 212.931 kb on + strand, within GFF2160 at 212.931 kb on + strand, within GFF2160 at 212.931 kb on + strand, within GFF2160 at 212.931 kb on + strand, within GFF2160 at 212.931 kb on + strand, within GFF2160 at 212.931 kb on + strand, within GFF2160 at 212.931 kb on + strand, within GFF2160 at 212.932 kb on - strand, within GFF2160 at 212.932 kb on - strand, within GFF2160 at 212.932 kb on - strand, within GFF2160 at 212.932 kb on - strand, within GFF2160 at 212.932 kb on - strand, within GFF2160 at 212.932 kb on - strand, within GFF2160 at 212.932 kb on - strand, within GFF2160 at 212.932 kb on - strand, within GFF2160 at 212.932 kb on - strand, within GFF2160 at 212.932 kb on - strand, within GFF2160 at 212.932 kb on - strand, within GFF2160 at 212.932 kb on - strand, within GFF2160 at 212.932 kb on - strand, within GFF2160 at 212.946 kb on + strand, within GFF2160 at 212.946 kb on + strand, within GFF2160 at 212.947 kb on - strand, within GFF2160 at 213.413 kb on + strand at 213.414 kb on - strand at 213.632 kb on + strand, within GFF2161 at 213.932 kb on + strand, within GFF2161 at 213.932 kb on + strand, within GFF2161 at 213.932 kb on + strand, within GFF2161 at 213.933 kb on - strand, within GFF2161 at 213.933 kb on - strand, within GFF2161 at 213.951 kb on - strand, within GFF2161 at 214.236 kb on - strand, within GFF2161
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 211,996 + +0.9 211,996 + -1.0 211,996 + -1.7 211,997 - +0.1 211,997 - +0.1 212,018 + GFF2159 0.10 -0.1 212,019 - GFF2159 0.11 -1.8 212,245 + GFF2159 0.56 -1.3 212,245 + GFF2159 0.56 -0.7 212,245 + GFF2159 0.56 -0.0 212,245 + GFF2159 0.56 -2.3 212,246 - GFF2159 0.56 -0.5 212,246 - GFF2159 0.56 -1.0 212,246 - GFF2159 0.56 -0.4 212,246 - GFF2159 0.56 -0.5 212,246 - GFF2159 0.56 -1.4 212,461 + +0.1 212,461 + -0.3 212,461 + -0.6 212,461 + -1.0 212,461 + +0.7 212,461 + -0.5 212,461 + -0.7 212,461 + -0.6 212,461 + +0.1 212,461 + -0.1 212,462 - +0.1 212,462 - -0.2 212,462 - -0.1 212,462 - +1.0 212,462 - -0.0 212,462 - +1.1 212,462 - -0.3 212,462 - -0.8 212,462 - -0.7 212,462 - +0.1 212,462 - -0.9 212,462 - -0.9 212,469 + -0.3 212,469 + -0.6 212,469 + +0.3 212,469 + +0.3 212,469 + -0.4 212,470 - -0.4 212,470 - +0.6 212,470 - +1.3 212,470 - -1.4 212,838 + GFF2160 0.39 -0.9 212,838 + GFF2160 0.39 +0.2 212,839 - GFF2160 0.39 -2.5 212,931 + GFF2160 0.49 +0.9 212,931 + GFF2160 0.49 +0.7 212,931 + GFF2160 0.49 +0.0 212,931 + GFF2160 0.49 +0.4 212,931 + GFF2160 0.49 +2.1 212,931 + GFF2160 0.49 -1.4 212,931 + GFF2160 0.49 -0.2 212,932 - GFF2160 0.49 +0.0 212,932 - GFF2160 0.49 +0.1 212,932 - GFF2160 0.49 -0.7 212,932 - GFF2160 0.49 -0.6 212,932 - GFF2160 0.49 +0.3 212,932 - GFF2160 0.49 -0.8 212,932 - GFF2160 0.49 +1.3 212,932 - GFF2160 0.49 +0.0 212,932 - GFF2160 0.49 -0.1 212,932 - GFF2160 0.49 +0.7 212,932 - GFF2160 0.49 -1.4 212,932 - GFF2160 0.49 -0.0 212,932 - GFF2160 0.49 -0.1 212,946 + GFF2160 0.50 +0.6 212,946 + GFF2160 0.50 +1.7 212,947 - GFF2160 0.50 +0.3 213,413 + -0.5 213,414 - -0.1 213,632 + GFF2161 0.16 +2.7 213,932 + GFF2161 0.40 -0.4 213,932 + GFF2161 0.40 -2.5 213,932 + GFF2161 0.40 +0.2 213,933 - GFF2161 0.40 +3.0 213,933 - GFF2161 0.40 +0.3 213,951 - GFF2161 0.41 -0.6 214,236 - GFF2161 0.63 -2.0
Or see this region's nucleotide sequence