Strain Fitness in Variovorax sp. SCN45 around GFF215

Experiment: M9 with Supernatant from Cyptococcus

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF213 and GFF214 are separated by 99 nucleotidesGFF214 and GFF215 are separated by 15 nucleotidesGFF215 and GFF216 are separated by 13 nucleotidesGFF216 and GFF217 are separated by 86 nucleotides GFF213 - tmRNA-binding protein SmpB, at 238,538 to 239,014 GFF213 GFF214 - Putative transmembrane protein, at 239,114 to 239,494 GFF214 GFF215 - RNA polymerase sigma-54 factor RpoN, at 239,510 to 240,025 GFF215 GFF216 - Transmembrane regulator protein PrtR, at 240,039 to 240,863 GFF216 GFF217 - Orf21; putative lipoprotein, at 240,950 to 241,333 GFF217 Position (kb) 239 240 241Strain fitness (log2 ratio) -2 -1 0 1 2at 238.558 kb on + strandat 238.558 kb on + strandat 238.558 kb on + strandat 238.558 kb on + strandat 238.558 kb on + strandat 238.558 kb on + strandat 238.558 kb on + strandat 238.558 kb on + strandat 238.558 kb on + strandat 238.559 kb on - strandat 238.559 kb on - strandat 238.559 kb on - strandat 238.559 kb on - strandat 238.559 kb on - strandat 238.559 kb on - strandat 238.559 kb on - strandat 238.559 kb on - strandat 238.596 kb on - strand, within GFF213at 238.596 kb on - strand, within GFF213at 238.596 kb on - strand, within GFF213at 238.601 kb on + strand, within GFF213at 238.601 kb on + strand, within GFF213at 238.601 kb on + strand, within GFF213at 238.602 kb on - strand, within GFF213at 238.602 kb on - strand, within GFF213at 238.602 kb on - strand, within GFF213at 238.694 kb on + strand, within GFF213at 238.695 kb on - strand, within GFF213at 238.856 kb on + strand, within GFF213at 238.856 kb on + strand, within GFF213at 238.856 kb on + strand, within GFF213at 238.856 kb on + strand, within GFF213at 239.039 kb on - strandat 239.039 kb on - strandat 239.039 kb on - strandat 239.243 kb on + strand, within GFF214at 239.243 kb on + strand, within GFF214at 239.243 kb on + strand, within GFF214at 239.243 kb on + strand, within GFF214at 239.243 kb on + strand, within GFF214at 239.244 kb on - strand, within GFF214at 239.244 kb on - strand, within GFF214at 239.244 kb on - strand, within GFF214at 239.244 kb on - strand, within GFF214at 239.244 kb on - strand, within GFF214at 239.414 kb on + strand, within GFF214at 239.414 kb on + strand, within GFF214at 239.414 kb on + strand, within GFF214at 239.414 kb on + strand, within GFF214at 239.415 kb on - strand, within GFF214at 239.415 kb on - strand, within GFF214at 239.417 kb on + strand, within GFF214at 239.417 kb on + strand, within GFF214at 239.417 kb on + strand, within GFF214at 239.418 kb on - strand, within GFF214at 239.418 kb on - strand, within GFF214at 239.418 kb on - strand, within GFF214at 239.418 kb on - strand, within GFF214at 239.418 kb on - strand, within GFF214at 239.418 kb on - strand, within GFF214at 239.556 kb on - strandat 239.888 kb on + strand, within GFF215at 239.888 kb on + strand, within GFF215at 239.889 kb on - strand, within GFF215at 239.889 kb on - strand, within GFF215at 239.889 kb on - strand, within GFF215at 239.889 kb on - strand, within GFF215at 240.459 kb on + strand, within GFF216at 240.459 kb on + strand, within GFF216at 240.459 kb on + strand, within GFF216at 240.460 kb on - strand, within GFF216at 240.460 kb on - strand, within GFF216at 240.579 kb on + strand, within GFF216at 240.579 kb on + strand, within GFF216at 240.579 kb on + strand, within GFF216at 240.579 kb on + strand, within GFF216at 240.580 kb on - strand, within GFF216at 240.580 kb on - strand, within GFF216at 240.580 kb on - strand, within GFF216at 240.580 kb on - strand, within GFF216at 240.580 kb on - strand, within GFF216at 240.580 kb on - strand, within GFF216at 240.580 kb on - strand, within GFF216at 240.672 kb on + strand, within GFF216at 240.672 kb on + strand, within GFF216at 240.672 kb on + strand, within GFF216at 240.672 kb on + strand, within GFF216at 240.672 kb on + strand, within GFF216at 240.672 kb on + strand, within GFF216at 240.672 kb on + strand, within GFF216at 240.673 kb on - strand, within GFF216at 240.673 kb on - strand, within GFF216at 240.673 kb on - strand, within GFF216at 240.673 kb on - strand, within GFF216at 240.765 kb on + strand, within GFF216at 240.765 kb on + strand, within GFF216at 240.765 kb on + strand, within GFF216at 240.765 kb on + strand, within GFF216at 240.765 kb on + strand, within GFF216at 240.766 kb on - strand, within GFF216at 240.766 kb on - strand, within GFF216at 240.846 kb on + strandat 240.861 kb on + strandat 240.861 kb on + strandat 240.872 kb on + strandat 240.873 kb on - strandat 240.873 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus
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238,558 + -0.6
238,558 + +0.7
238,558 + -0.7
238,558 + -0.4
238,558 + +0.1
238,558 + +1.3
238,558 + +1.3
238,558 + -2.2
238,558 + -1.4
238,559 - +0.4
238,559 - -0.6
238,559 - -1.1
238,559 - -0.1
238,559 - +0.4
238,559 - +0.1
238,559 - -0.4
238,559 - +0.7
238,596 - GFF213 0.12 +0.0
238,596 - GFF213 0.12 +1.3
238,596 - GFF213 0.12 -1.1
238,601 + GFF213 0.13 -1.6
238,601 + GFF213 0.13 +1.3
238,601 + GFF213 0.13 -1.3
238,602 - GFF213 0.13 -0.7
238,602 - GFF213 0.13 -1.1
238,602 - GFF213 0.13 -0.3
238,694 + GFF213 0.33 -0.7
238,695 - GFF213 0.33 -0.1
238,856 + GFF213 0.67 -0.7
238,856 + GFF213 0.67 -0.7
238,856 + GFF213 0.67 +0.3
238,856 + GFF213 0.67 -1.3
239,039 - +0.5
239,039 - +0.8
239,039 - +1.0
239,243 + GFF214 0.34 -0.1
239,243 + GFF214 0.34 +0.2
239,243 + GFF214 0.34 -1.0
239,243 + GFF214 0.34 -0.7
239,243 + GFF214 0.34 -0.6
239,244 - GFF214 0.34 -0.1
239,244 - GFF214 0.34 -1.3
239,244 - GFF214 0.34 -0.7
239,244 - GFF214 0.34 -0.3
239,244 - GFF214 0.34 -0.6
239,414 + GFF214 0.79 +0.3
239,414 + GFF214 0.79 +0.5
239,414 + GFF214 0.79 -0.8
239,414 + GFF214 0.79 +1.0
239,415 - GFF214 0.79 +0.3
239,415 - GFF214 0.79 -0.7
239,417 + GFF214 0.80 -1.2
239,417 + GFF214 0.80 +0.1
239,417 + GFF214 0.80 -1.6
239,418 - GFF214 0.80 +1.0
239,418 - GFF214 0.80 -0.4
239,418 - GFF214 0.80 +1.3
239,418 - GFF214 0.80 -0.1
239,418 - GFF214 0.80 -0.1
239,418 - GFF214 0.80 +0.3
239,556 - -0.4
239,888 + GFF215 0.73 +0.8
239,888 + GFF215 0.73 +0.5
239,889 - GFF215 0.73 -0.6
239,889 - GFF215 0.73 -0.2
239,889 - GFF215 0.73 -1.1
239,889 - GFF215 0.73 +0.6
240,459 + GFF216 0.51 -1.1
240,459 + GFF216 0.51 +0.2
240,459 + GFF216 0.51 +1.3
240,460 - GFF216 0.51 -0.0
240,460 - GFF216 0.51 -1.3
240,579 + GFF216 0.65 +0.4
240,579 + GFF216 0.65 -0.1
240,579 + GFF216 0.65 -1.2
240,579 + GFF216 0.65 +1.0
240,580 - GFF216 0.66 -0.9
240,580 - GFF216 0.66 -0.7
240,580 - GFF216 0.66 +0.2
240,580 - GFF216 0.66 -1.1
240,580 - GFF216 0.66 +0.4
240,580 - GFF216 0.66 -0.7
240,580 - GFF216 0.66 +0.1
240,672 + GFF216 0.77 -0.2
240,672 + GFF216 0.77 -0.7
240,672 + GFF216 0.77 -0.3
240,672 + GFF216 0.77 -0.2
240,672 + GFF216 0.77 -1.2
240,672 + GFF216 0.77 -1.1
240,672 + GFF216 0.77 -1.2
240,673 - GFF216 0.77 -2.4
240,673 - GFF216 0.77 -0.1
240,673 - GFF216 0.77 +0.7
240,673 - GFF216 0.77 -0.3
240,765 + GFF216 0.88 -2.2
240,765 + GFF216 0.88 +0.5
240,765 + GFF216 0.88 -0.1
240,765 + GFF216 0.88 -0.3
240,765 + GFF216 0.88 -0.1
240,766 - GFF216 0.88 +0.8
240,766 - GFF216 0.88 -1.4
240,846 + +0.8
240,861 + -2.1
240,861 + +0.5
240,872 + +1.8
240,873 - -0.9
240,873 - +0.1

Or see this region's nucleotide sequence