Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2125 and GFF2126 are separated by 321 nucleotides GFF2126 and GFF2127 overlap by 4 nucleotides GFF2127 and GFF2128 are separated by 30 nucleotides GFF2128 and GFF2129 are separated by 96 nucleotides
GFF2125 - hypothetical protein, at 175,001 to 175,351
GFF2125
GFF2126 - hypothetical protein, at 175,673 to 176,338
GFF2126
GFF2127 - Type II/IV secretion system secretin RcpA/CpaC, associated with Flp pilus assembly, at 176,335 to 177,612
GFF2127
GFF2128 - Enoyl-CoA hydratase (EC 4.2.1.17), at 177,643 to 178,422
GFF2128
GFF2129 - small multidrug resistance family (SMR) protein, at 178,519 to 178,854
GFF2129
Position (kb) 176
177
178 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 175.392 kb on + strand at 175.392 kb on + strand at 175.444 kb on + strand at 175.444 kb on + strand at 175.444 kb on + strand at 175.445 kb on - strand at 175.445 kb on - strand at 175.648 kb on + strand at 175.648 kb on + strand at 175.648 kb on + strand at 175.649 kb on - strand at 175.790 kb on + strand, within GFF2126 at 175.791 kb on - strand, within GFF2126 at 176.783 kb on + strand, within GFF2127 at 176.926 kb on - strand, within GFF2127 at 176.926 kb on - strand, within GFF2127 at 176.926 kb on - strand, within GFF2127 at 176.926 kb on - strand, within GFF2127 at 177.062 kb on + strand, within GFF2127 at 177.062 kb on + strand, within GFF2127 at 177.063 kb on - strand, within GFF2127 at 177.086 kb on - strand, within GFF2127 at 177.086 kb on - strand, within GFF2127 at 177.086 kb on - strand, within GFF2127 at 177.086 kb on - strand, within GFF2127 at 177.086 kb on - strand, within GFF2127 at 177.196 kb on + strand, within GFF2127 at 177.196 kb on + strand, within GFF2127 at 177.473 kb on - strand, within GFF2127 at 177.821 kb on + strand, within GFF2128 at 178.172 kb on + strand, within GFF2128 at 178.172 kb on + strand, within GFF2128 at 178.172 kb on + strand, within GFF2128 at 178.172 kb on + strand, within GFF2128 at 178.172 kb on + strand, within GFF2128 at 178.172 kb on + strand, within GFF2128 at 178.172 kb on + strand, within GFF2128 at 178.184 kb on + strand, within GFF2128 at 178.184 kb on + strand, within GFF2128 at 178.184 kb on + strand, within GFF2128 at 178.184 kb on + strand, within GFF2128 at 178.184 kb on + strand, within GFF2128 at 178.185 kb on - strand, within GFF2128 at 178.185 kb on - strand, within GFF2128 at 178.185 kb on - strand, within GFF2128 at 178.185 kb on - strand, within GFF2128 at 178.185 kb on - strand, within GFF2128 at 178.202 kb on + strand, within GFF2128 at 178.202 kb on + strand, within GFF2128 at 178.202 kb on + strand, within GFF2128 at 178.202 kb on + strand, within GFF2128 at 178.202 kb on + strand, within GFF2128 at 178.203 kb on - strand, within GFF2128 at 178.203 kb on - strand, within GFF2128 at 178.203 kb on - strand, within GFF2128 at 178.203 kb on - strand, within GFF2128 at 178.415 kb on + strand at 178.415 kb on + strand at 178.416 kb on - strand at 178.461 kb on - strand at 178.461 kb on - strand at 178.462 kb on + strand at 178.462 kb on + strand at 178.462 kb on + strand at 178.463 kb on - strand at 178.506 kb on + strand at 178.507 kb on - strand at 178.507 kb on - strand at 178.507 kb on - strand at 178.531 kb on + strand at 178.531 kb on + strand at 178.531 kb on + strand at 178.531 kb on + strand at 178.531 kb on + strand at 178.531 kb on + strand at 178.531 kb on + strand at 178.531 kb on + strand at 178.531 kb on + strand at 178.532 kb on - strand at 178.537 kb on + strand at 178.537 kb on + strand at 178.538 kb on - strand at 178.538 kb on - strand at 178.538 kb on - strand at 178.538 kb on - strand at 178.538 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 175,392 + +0.4 175,392 + +0.0 175,444 + -2.0 175,444 + +1.0 175,444 + -1.0 175,445 - +0.1 175,445 - -0.0 175,648 + -1.8 175,648 + +1.3 175,648 + -0.2 175,649 - -0.6 175,790 + GFF2126 0.18 -1.2 175,791 - GFF2126 0.18 -0.3 176,783 + GFF2127 0.35 +0.5 176,926 - GFF2127 0.46 +0.8 176,926 - GFF2127 0.46 -1.8 176,926 - GFF2127 0.46 -0.0 176,926 - GFF2127 0.46 -2.2 177,062 + GFF2127 0.57 -0.5 177,062 + GFF2127 0.57 -1.3 177,063 - GFF2127 0.57 -2.9 177,086 - GFF2127 0.59 +0.1 177,086 - GFF2127 0.59 -0.9 177,086 - GFF2127 0.59 -0.4 177,086 - GFF2127 0.59 -0.5 177,086 - GFF2127 0.59 -0.7 177,196 + GFF2127 0.67 -0.4 177,196 + GFF2127 0.67 +0.4 177,473 - GFF2127 0.89 -0.5 177,821 + GFF2128 0.23 +0.3 178,172 + GFF2128 0.68 -0.5 178,172 + GFF2128 0.68 -2.5 178,172 + GFF2128 0.68 +0.7 178,172 + GFF2128 0.68 -0.5 178,172 + GFF2128 0.68 -0.6 178,172 + GFF2128 0.68 -1.2 178,172 + GFF2128 0.68 -1.0 178,184 + GFF2128 0.69 -0.1 178,184 + GFF2128 0.69 -0.4 178,184 + GFF2128 0.69 -0.2 178,184 + GFF2128 0.69 -0.9 178,184 + GFF2128 0.69 -2.0 178,185 - GFF2128 0.69 -0.3 178,185 - GFF2128 0.69 +0.1 178,185 - GFF2128 0.69 -0.4 178,185 - GFF2128 0.69 +0.7 178,185 - GFF2128 0.69 -2.4 178,202 + GFF2128 0.72 -0.8 178,202 + GFF2128 0.72 -1.1 178,202 + GFF2128 0.72 -2.1 178,202 + GFF2128 0.72 -1.0 178,202 + GFF2128 0.72 -0.4 178,203 - GFF2128 0.72 -0.5 178,203 - GFF2128 0.72 +0.0 178,203 - GFF2128 0.72 -0.6 178,203 - GFF2128 0.72 -1.1 178,415 + -0.3 178,415 + -0.3 178,416 - +0.6 178,461 - -1.0 178,461 - -0.7 178,462 + +0.2 178,462 + -0.3 178,462 + -0.3 178,463 - +0.2 178,506 + +0.5 178,507 - -0.5 178,507 - -2.6 178,507 - +0.2 178,531 + +0.4 178,531 + +0.9 178,531 + -1.5 178,531 + -0.7 178,531 + +1.4 178,531 + -1.8 178,531 + -0.9 178,531 + -1.4 178,531 + -0.6 178,532 - +0.0 178,537 + -0.1 178,537 + -0.4 178,538 - -2.3 178,538 - -1.0 178,538 - +0.1 178,538 - +0.0 178,538 - +1.1
Or see this region's nucleotide sequence