Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2090 and GFF2091 are separated by 7 nucleotides GFF2091 and GFF2092 overlap by 4 nucleotides GFF2092 and GFF2093 are separated by 2 nucleotides
GFF2090 - Holliday junction ATP-dependent DNA helicase RuvB (EC 3.6.4.12), at 137,923 to 138,987
GFF2090
GFF2091 - Cobalt/zinc/cadmium resistance protein CzcD, at 138,995 to 139,948
GFF2091
GFF2092 - hypothetical protein, at 139,945 to 140,064
GFF2092
GFF2093 - Fatty acid hydroxylase family (carotene hydroxylase/sterol desaturase), at 140,067 to 141,809
GFF2093
Position (kb)
138
139
140 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 138.040 kb on + strand, within GFF2090 at 138.041 kb on - strand, within GFF2090 at 138.346 kb on + strand, within GFF2090 at 138.347 kb on - strand, within GFF2090 at 138.347 kb on - strand, within GFF2090 at 138.499 kb on + strand, within GFF2090 at 138.500 kb on - strand, within GFF2090 at 138.500 kb on - strand, within GFF2090 at 138.500 kb on - strand, within GFF2090 at 138.500 kb on - strand, within GFF2090 at 138.877 kb on + strand, within GFF2090 at 138.877 kb on + strand, within GFF2090 at 139.230 kb on - strand, within GFF2091 at 139.535 kb on + strand, within GFF2091 at 139.535 kb on + strand, within GFF2091 at 139.535 kb on + strand, within GFF2091 at 139.823 kb on + strand, within GFF2091 at 139.823 kb on + strand, within GFF2091 at 139.824 kb on - strand, within GFF2091 at 139.946 kb on + strand at 139.946 kb on + strand at 140.061 kb on - strand at 140.061 kb on - strand at 140.113 kb on + strand at 140.113 kb on + strand at 140.113 kb on + strand at 140.113 kb on + strand at 140.114 kb on - strand at 140.114 kb on - strand at 140.114 kb on - strand at 140.116 kb on + strand at 140.116 kb on + strand at 140.116 kb on + strand at 140.116 kb on + strand at 140.116 kb on + strand at 140.116 kb on + strand at 140.117 kb on - strand at 140.117 kb on - strand at 140.117 kb on - strand at 140.359 kb on + strand, within GFF2093 at 140.359 kb on + strand, within GFF2093 at 140.359 kb on + strand, within GFF2093 at 140.359 kb on + strand, within GFF2093 at 140.359 kb on + strand, within GFF2093 at 140.359 kb on + strand, within GFF2093 at 140.359 kb on + strand, within GFF2093 at 140.359 kb on + strand, within GFF2093 at 140.359 kb on + strand, within GFF2093 at 140.359 kb on + strand, within GFF2093 at 140.359 kb on + strand, within GFF2093 at 140.359 kb on + strand, within GFF2093 at 140.360 kb on - strand, within GFF2093 at 140.360 kb on - strand, within GFF2093 at 140.360 kb on - strand, within GFF2093 at 140.360 kb on - strand, within GFF2093 at 140.360 kb on - strand, within GFF2093 at 140.360 kb on - strand, within GFF2093 at 140.360 kb on - strand, within GFF2093 at 140.360 kb on - strand, within GFF2093 at 140.360 kb on - strand, within GFF2093 at 140.360 kb on - strand, within GFF2093 at 140.443 kb on + strand, within GFF2093 at 140.443 kb on + strand, within GFF2093 at 140.443 kb on + strand, within GFF2093 at 140.443 kb on + strand, within GFF2093 at 140.443 kb on + strand, within GFF2093 at 140.443 kb on + strand, within GFF2093 at 140.444 kb on - strand, within GFF2093 at 140.444 kb on - strand, within GFF2093 at 140.511 kb on + strand, within GFF2093 at 140.511 kb on + strand, within GFF2093 at 140.511 kb on + strand, within GFF2093 at 140.511 kb on + strand, within GFF2093 at 140.512 kb on - strand, within GFF2093 at 140.512 kb on - strand, within GFF2093 at 140.512 kb on - strand, within GFF2093 at 140.512 kb on - strand, within GFF2093 at 140.641 kb on + strand, within GFF2093 at 140.641 kb on + strand, within GFF2093 at 140.641 kb on + strand, within GFF2093 at 140.641 kb on + strand, within GFF2093 at 140.641 kb on + strand, within GFF2093 at 140.641 kb on + strand, within GFF2093 at 140.641 kb on + strand, within GFF2093 at 140.642 kb on - strand, within GFF2093 at 140.642 kb on - strand, within GFF2093 at 140.642 kb on - strand, within GFF2093 at 140.706 kb on + strand, within GFF2093 at 140.706 kb on + strand, within GFF2093 at 140.706 kb on + strand, within GFF2093 at 140.706 kb on + strand, within GFF2093 at 140.707 kb on - strand, within GFF2093 at 140.707 kb on - strand, within GFF2093
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 138,040 + GFF2090 0.11 -0.5 138,041 - GFF2090 0.11 +0.1 138,346 + GFF2090 0.40 -2.6 138,347 - GFF2090 0.40 -0.1 138,347 - GFF2090 0.40 +0.7 138,499 + GFF2090 0.54 -0.9 138,500 - GFF2090 0.54 -0.1 138,500 - GFF2090 0.54 -1.1 138,500 - GFF2090 0.54 -1.2 138,500 - GFF2090 0.54 +0.7 138,877 + GFF2090 0.90 -2.9 138,877 + GFF2090 0.90 -0.9 139,230 - GFF2091 0.25 +0.7 139,535 + GFF2091 0.57 +0.8 139,535 + GFF2091 0.57 +0.3 139,535 + GFF2091 0.57 +1.4 139,823 + GFF2091 0.87 +0.2 139,823 + GFF2091 0.87 -0.1 139,824 - GFF2091 0.87 -0.1 139,946 + -0.4 139,946 + +0.5 140,061 - +0.7 140,061 - -0.4 140,113 + -0.8 140,113 + -0.1 140,113 + -0.3 140,113 + -1.1 140,114 - -0.5 140,114 - -1.8 140,114 - -0.8 140,116 + +0.1 140,116 + -1.1 140,116 + -0.2 140,116 + -0.9 140,116 + +0.7 140,116 + +0.5 140,117 - -0.9 140,117 - -0.4 140,117 - +0.4 140,359 + GFF2093 0.17 -0.2 140,359 + GFF2093 0.17 +1.1 140,359 + GFF2093 0.17 -0.0 140,359 + GFF2093 0.17 +0.4 140,359 + GFF2093 0.17 -0.4 140,359 + GFF2093 0.17 +0.7 140,359 + GFF2093 0.17 -0.4 140,359 + GFF2093 0.17 +0.0 140,359 + GFF2093 0.17 -0.3 140,359 + GFF2093 0.17 +0.6 140,359 + GFF2093 0.17 -0.3 140,359 + GFF2093 0.17 +2.7 140,360 - GFF2093 0.17 -1.5 140,360 - GFF2093 0.17 +0.3 140,360 - GFF2093 0.17 +0.8 140,360 - GFF2093 0.17 +4.5 140,360 - GFF2093 0.17 -1.0 140,360 - GFF2093 0.17 +0.1 140,360 - GFF2093 0.17 -0.6 140,360 - GFF2093 0.17 -0.2 140,360 - GFF2093 0.17 +0.5 140,360 - GFF2093 0.17 -0.2 140,443 + GFF2093 0.22 +1.0 140,443 + GFF2093 0.22 -0.3 140,443 + GFF2093 0.22 +0.3 140,443 + GFF2093 0.22 -0.1 140,443 + GFF2093 0.22 +1.7 140,443 + GFF2093 0.22 +0.0 140,444 - GFF2093 0.22 -0.5 140,444 - GFF2093 0.22 +2.3 140,511 + GFF2093 0.25 +0.7 140,511 + GFF2093 0.25 +0.0 140,511 + GFF2093 0.25 +0.4 140,511 + GFF2093 0.25 +1.1 140,512 - GFF2093 0.26 -0.1 140,512 - GFF2093 0.26 -0.4 140,512 - GFF2093 0.26 +0.2 140,512 - GFF2093 0.26 -0.2 140,641 + GFF2093 0.33 +0.1 140,641 + GFF2093 0.33 -0.3 140,641 + GFF2093 0.33 -0.0 140,641 + GFF2093 0.33 -0.1 140,641 + GFF2093 0.33 -0.6 140,641 + GFF2093 0.33 -1.3 140,641 + GFF2093 0.33 -0.1 140,642 - GFF2093 0.33 +0.1 140,642 - GFF2093 0.33 -3.3 140,642 - GFF2093 0.33 +0.4 140,706 + GFF2093 0.37 -0.1 140,706 + GFF2093 0.37 -0.2 140,706 + GFF2093 0.37 -1.4 140,706 + GFF2093 0.37 -0.1 140,707 - GFF2093 0.37 -0.4 140,707 - GFF2093 0.37 +0.9
Or see this region's nucleotide sequence