Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1940 and GFF1941 are separated by 8 nucleotides GFF1941 and GFF1942 are separated by 71 nucleotides GFF1942 and GFF1943 are separated by 51 nucleotides
GFF1940 - Adenylosuccinate synthetase-like protein, at 633,764 to 635,107
GFF1940
GFF1941 - hypothetical protein, at 635,116 to 635,544
GFF1941
GFF1942 - no description, at 635,616 to 635,741
GFF1942
GFF1943 - DNA polymerase III alpha subunit (EC 2.7.7.7), at 635,793 to 638,579
GFF1943
Position (kb) 635
636 Strain fitness (log2 ratio) -1
0
1 at 634.778 kb on + strand, within GFF1940 at 634.976 kb on + strand at 634.977 kb on - strand at 634.977 kb on - strand at 635.125 kb on + strand at 635.125 kb on + strand at 635.398 kb on + strand, within GFF1941 at 635.399 kb on - strand, within GFF1941 at 635.613 kb on + strand at 635.614 kb on - strand at 635.617 kb on + strand at 635.618 kb on - strand at 635.618 kb on - strand at 635.618 kb on - strand at 635.814 kb on + strand at 635.814 kb on + strand at 635.814 kb on + strand at 635.829 kb on - strand at 636.088 kb on - strand, within GFF1943 at 636.123 kb on + strand, within GFF1943 at 636.123 kb on + strand, within GFF1943 at 636.124 kb on - strand, within GFF1943
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 634,778 + GFF1940 0.75 +1.7 634,976 + -0.4 634,977 - -0.3 634,977 - +0.1 635,125 + +0.0 635,125 + +0.4 635,398 + GFF1941 0.66 -1.3 635,399 - GFF1941 0.66 -1.0 635,613 + -1.1 635,614 - -0.2 635,617 + -0.4 635,618 - +0.7 635,618 - -0.5 635,618 - +0.5 635,814 + +0.0 635,814 + -1.0 635,814 + -1.7 635,829 - -1.0 636,088 - GFF1943 0.11 -1.0 636,123 + GFF1943 0.12 -0.6 636,123 + GFF1943 0.12 -0.6 636,124 - GFF1943 0.12 -0.9
Or see this region's nucleotide sequence