Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1647 and GFF1648 are separated by 128 nucleotides GFF1648 and GFF1649 are separated by 159 nucleotides GFF1649 and GFF1650 are separated by 104 nucleotides
GFF1647 - Acyl-homoserine lactone acylase PvdQ (EC 3.5.1.-), quorum-quenching, at 285,963 to 288,191
GFF1647
GFF1648 - Ligand-binding SRPBCC domain protein family, at 288,320 to 288,766
GFF1648
GFF1649 - hypothetical protein, at 288,926 to 289,591
GFF1649
GFF1650 - NmrA-like, at 289,696 to 290,781
GFF1650
Position (kb) 288
289 Strain fitness (log2 ratio) -2
-1
0
1 at 287.428 kb on + strand, within GFF1647 at 287.429 kb on - strand, within GFF1647 at 287.429 kb on - strand, within GFF1647 at 287.452 kb on + strand, within GFF1647 at 287.650 kb on + strand, within GFF1647 at 287.686 kb on + strand, within GFF1647 at 287.686 kb on + strand, within GFF1647 at 287.794 kb on + strand, within GFF1647 at 287.795 kb on - strand, within GFF1647 at 287.795 kb on - strand, within GFF1647 at 287.795 kb on - strand, within GFF1647 at 287.795 kb on - strand, within GFF1647 at 287.962 kb on + strand, within GFF1647 at 287.984 kb on - strand at 288.290 kb on - strand at 288.439 kb on - strand, within GFF1648 at 288.519 kb on + strand, within GFF1648 at 288.520 kb on - strand, within GFF1648 at 288.520 kb on - strand, within GFF1648 at 288.552 kb on + strand, within GFF1648 at 288.553 kb on - strand, within GFF1648 at 288.553 kb on - strand, within GFF1648 at 288.574 kb on + strand, within GFF1648 at 288.575 kb on - strand, within GFF1648 at 288.708 kb on + strand, within GFF1648 at 288.807 kb on + strand at 288.808 kb on - strand at 288.883 kb on + strand at 288.883 kb on + strand at 288.884 kb on - strand at 288.983 kb on + strand at 288.983 kb on + strand at 288.983 kb on + strand at 288.983 kb on + strand at 288.983 kb on + strand at 288.983 kb on + strand at 288.984 kb on - strand at 288.984 kb on - strand at 288.984 kb on - strand at 288.984 kb on - strand at 288.984 kb on - strand at 288.984 kb on - strand at 288.984 kb on - strand at 288.984 kb on - strand at 289.158 kb on - strand, within GFF1649 at 289.276 kb on + strand, within GFF1649 at 289.287 kb on + strand, within GFF1649 at 289.287 kb on + strand, within GFF1649 at 289.287 kb on + strand, within GFF1649 at 289.288 kb on - strand, within GFF1649 at 289.288 kb on - strand, within GFF1649 at 289.288 kb on - strand, within GFF1649 at 289.288 kb on - strand, within GFF1649 at 289.489 kb on + strand, within GFF1649 at 289.589 kb on + strand at 289.589 kb on + strand at 289.589 kb on + strand at 289.590 kb on - strand at 289.719 kb on + strand at 289.720 kb on - strand at 289.753 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 287,428 + GFF1647 0.66 +0.9 287,429 - GFF1647 0.66 +0.3 287,429 - GFF1647 0.66 -1.6 287,452 + GFF1647 0.67 +0.9 287,650 + GFF1647 0.76 +0.6 287,686 + GFF1647 0.77 +0.9 287,686 + GFF1647 0.77 -0.8 287,794 + GFF1647 0.82 +0.2 287,795 - GFF1647 0.82 +0.6 287,795 - GFF1647 0.82 +0.5 287,795 - GFF1647 0.82 -0.0 287,795 - GFF1647 0.82 +0.8 287,962 + GFF1647 0.90 -0.7 287,984 - -0.2 288,290 - -0.9 288,439 - GFF1648 0.27 +0.7 288,519 + GFF1648 0.45 -2.4 288,520 - GFF1648 0.45 -1.0 288,520 - GFF1648 0.45 -0.5 288,552 + GFF1648 0.52 +0.3 288,553 - GFF1648 0.52 +0.3 288,553 - GFF1648 0.52 +0.2 288,574 + GFF1648 0.57 +1.1 288,575 - GFF1648 0.57 -1.1 288,708 + GFF1648 0.87 -1.0 288,807 + -0.4 288,808 - -0.1 288,883 + -1.4 288,883 + -0.3 288,884 - -2.1 288,983 + -0.9 288,983 + -0.2 288,983 + -0.2 288,983 + -0.4 288,983 + +1.7 288,983 + +1.7 288,984 - -0.5 288,984 - +0.3 288,984 - -0.2 288,984 - -0.4 288,984 - +0.2 288,984 - -0.4 288,984 - +0.9 288,984 - -0.6 289,158 - GFF1649 0.35 +1.3 289,276 + GFF1649 0.53 -0.7 289,287 + GFF1649 0.54 -0.7 289,287 + GFF1649 0.54 -0.5 289,287 + GFF1649 0.54 -1.7 289,288 - GFF1649 0.54 -0.8 289,288 - GFF1649 0.54 -0.2 289,288 - GFF1649 0.54 +0.2 289,288 - GFF1649 0.54 +0.8 289,489 + GFF1649 0.85 -1.5 289,589 + -1.7 289,589 + -1.3 289,589 + -0.8 289,590 - -0.1 289,719 + -0.0 289,720 - -0.9 289,753 - +0.9
Or see this region's nucleotide sequence