Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1555 and GFF1556 are separated by 99 nucleotides GFF1556 and GFF1557 overlap by 4 nucleotides
GFF1555 - Transcriptional regulator, LysR family, at 153,567 to 154,460
GFF1555
GFF1556 - Tricarboxylate transport protein TctC, at 154,560 to 155,540
GFF1556
GFF1557 - ortholog of Bordetella pertussis (BX470248) BP3300, at 155,537 to 156,514
GFF1557
Position (kb)
154
155
156 Strain fitness (log2 ratio)
-2
-1
0
1 at 153.899 kb on - strand, within GFF1555 at 153.964 kb on + strand, within GFF1555 at 153.964 kb on + strand, within GFF1555 at 153.965 kb on - strand, within GFF1555 at 153.965 kb on - strand, within GFF1555 at 153.965 kb on - strand, within GFF1555 at 153.965 kb on - strand, within GFF1555 at 153.980 kb on + strand, within GFF1555 at 153.981 kb on - strand, within GFF1555 at 153.981 kb on - strand, within GFF1555 at 154.158 kb on + strand, within GFF1555 at 154.158 kb on + strand, within GFF1555 at 154.159 kb on - strand, within GFF1555 at 154.189 kb on + strand, within GFF1555 at 154.190 kb on - strand, within GFF1555 at 154.267 kb on - strand, within GFF1555 at 154.272 kb on + strand, within GFF1555 at 154.273 kb on - strand, within GFF1555 at 154.273 kb on - strand, within GFF1555 at 154.273 kb on - strand, within GFF1555 at 154.396 kb on - strand at 154.563 kb on + strand at 154.563 kb on + strand at 154.564 kb on - strand at 154.564 kb on - strand at 154.564 kb on - strand at 154.564 kb on - strand at 154.650 kb on + strand at 154.650 kb on + strand at 154.834 kb on - strand, within GFF1556 at 154.899 kb on + strand, within GFF1556 at 155.029 kb on - strand, within GFF1556 at 155.029 kb on - strand, within GFF1556 at 155.029 kb on - strand, within GFF1556 at 155.029 kb on - strand, within GFF1556 at 155.034 kb on + strand, within GFF1556 at 155.035 kb on - strand, within GFF1556 at 155.035 kb on - strand, within GFF1556 at 155.205 kb on + strand, within GFF1556 at 155.503 kb on - strand at 156.425 kb on + strand at 156.426 kb on - strand at 156.426 kb on - strand at 156.426 kb on - strand at 156.426 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 153,899 - GFF1555 0.37 +0.4 153,964 + GFF1555 0.44 -0.4 153,964 + GFF1555 0.44 +0.3 153,965 - GFF1555 0.45 +0.3 153,965 - GFF1555 0.45 +0.3 153,965 - GFF1555 0.45 -0.6 153,965 - GFF1555 0.45 -1.4 153,980 + GFF1555 0.46 +0.2 153,981 - GFF1555 0.46 +0.2 153,981 - GFF1555 0.46 -0.4 154,158 + GFF1555 0.66 +0.1 154,158 + GFF1555 0.66 -0.5 154,159 - GFF1555 0.66 -0.3 154,189 + GFF1555 0.70 +0.1 154,190 - GFF1555 0.70 -0.1 154,267 - GFF1555 0.78 -0.7 154,272 + GFF1555 0.79 -1.2 154,273 - GFF1555 0.79 -0.6 154,273 - GFF1555 0.79 -0.3 154,273 - GFF1555 0.79 -0.4 154,396 - +0.3 154,563 + -0.4 154,563 + -0.4 154,564 - -0.3 154,564 - -0.1 154,564 - +0.2 154,564 - +0.6 154,650 + -1.9 154,650 + -1.3 154,834 - GFF1556 0.28 +0.5 154,899 + GFF1556 0.35 -1.2 155,029 - GFF1556 0.48 +1.7 155,029 - GFF1556 0.48 +0.7 155,029 - GFF1556 0.48 -1.2 155,029 - GFF1556 0.48 +0.3 155,034 + GFF1556 0.48 +0.3 155,035 - GFF1556 0.48 +0.1 155,035 - GFF1556 0.48 +0.2 155,205 + GFF1556 0.66 +1.7 155,503 - -0.5 156,425 + -0.3 156,426 - +0.1 156,426 - -0.3 156,426 - +0.3 156,426 - +0.3
Or see this region's nucleotide sequence