Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1517 and GFF1518 are separated by 47 nucleotides GFF1518 and GFF1519 overlap by 4 nucleotides GFF1519 and GFF1520 are separated by 158 nucleotides GFF1520 and GFF1521 are separated by 25 nucleotides
GFF1517 - TRAP-type transport system, small permease component, predicted N-acetylneuraminate transporter, at 112,820 to 113,383
GFF1517
GFF1518 - 3-dehydroquinate dehydratase II (EC 4.2.1.10), at 113,431 to 113,901
GFF1518
GFF1519 - TRAP-type C4-dicarboxylate transport system, periplasmic component, at 113,898 to 114,899
GFF1519
GFF1520 - Transcriptional regulator, IclR family, at 115,058 to 115,840
GFF1520
GFF1521 - TRAP-type C4-dicarboxylate transport system, periplasmic component, at 115,866 to 116,885
GFF1521
Position (kb)
113
114
115 Strain fitness (log2 ratio)
-1
0
1
2 at 112.998 kb on + strand, within GFF1517 at 112.999 kb on - strand, within GFF1517 at 113.094 kb on + strand, within GFF1517 at 113.094 kb on + strand, within GFF1517 at 113.094 kb on + strand, within GFF1517 at 113.280 kb on + strand, within GFF1517 at 113.486 kb on + strand, within GFF1518 at 113.487 kb on - strand, within GFF1518 at 113.814 kb on - strand, within GFF1518 at 113.814 kb on - strand, within GFF1518 at 113.817 kb on + strand, within GFF1518 at 113.817 kb on + strand, within GFF1518 at 113.817 kb on + strand, within GFF1518 at 113.818 kb on - strand, within GFF1518 at 113.818 kb on - strand, within GFF1518 at 113.818 kb on - strand, within GFF1518 at 113.818 kb on - strand, within GFF1518 at 113.818 kb on - strand, within GFF1518 at 113.818 kb on - strand, within GFF1518 at 113.818 kb on - strand, within GFF1518 at 113.956 kb on + strand at 114.103 kb on + strand, within GFF1519 at 114.103 kb on + strand, within GFF1519 at 114.202 kb on + strand, within GFF1519 at 114.203 kb on - strand, within GFF1519 at 114.203 kb on - strand, within GFF1519 at 114.203 kb on - strand, within GFF1519 at 114.313 kb on + strand, within GFF1519 at 114.313 kb on + strand, within GFF1519 at 114.313 kb on + strand, within GFF1519 at 114.476 kb on - strand, within GFF1519 at 114.709 kb on + strand, within GFF1519 at 114.709 kb on + strand, within GFF1519 at 114.710 kb on - strand, within GFF1519 at 114.884 kb on + strand at 114.884 kb on + strand at 114.885 kb on - strand at 114.997 kb on - strand at 115.004 kb on + strand at 115.005 kb on - strand at 115.005 kb on - strand at 115.097 kb on + strand at 115.205 kb on + strand, within GFF1520 at 115.235 kb on + strand, within GFF1520 at 115.235 kb on + strand, within GFF1520 at 115.235 kb on + strand, within GFF1520 at 115.236 kb on - strand, within GFF1520 at 115.236 kb on - strand, within GFF1520 at 115.425 kb on - strand, within GFF1520 at 115.425 kb on - strand, within GFF1520 at 115.554 kb on - strand, within GFF1520 at 115.595 kb on + strand, within GFF1520 at 115.595 kb on + strand, within GFF1520 at 115.595 kb on + strand, within GFF1520 at 115.595 kb on + strand, within GFF1520 at 115.595 kb on + strand, within GFF1520 at 115.595 kb on + strand, within GFF1520 at 115.595 kb on + strand, within GFF1520 at 115.595 kb on + strand, within GFF1520 at 115.595 kb on + strand, within GFF1520 at 115.596 kb on - strand, within GFF1520 at 115.596 kb on - strand, within GFF1520 at 115.596 kb on - strand, within GFF1520 at 115.596 kb on - strand, within GFF1520 at 115.596 kb on - strand, within GFF1520 at 115.596 kb on - strand, within GFF1520 at 115.822 kb on - strand at 115.822 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 112,998 + GFF1517 0.32 +0.1 112,999 - GFF1517 0.32 +0.2 113,094 + GFF1517 0.49 -0.6 113,094 + GFF1517 0.49 +0.4 113,094 + GFF1517 0.49 +0.0 113,280 + GFF1517 0.82 +0.5 113,486 + GFF1518 0.12 -0.3 113,487 - GFF1518 0.12 +0.3 113,814 - GFF1518 0.81 -0.4 113,814 - GFF1518 0.81 -0.4 113,817 + GFF1518 0.82 +0.2 113,817 + GFF1518 0.82 -0.1 113,817 + GFF1518 0.82 +1.1 113,818 - GFF1518 0.82 -0.3 113,818 - GFF1518 0.82 -0.1 113,818 - GFF1518 0.82 -0.4 113,818 - GFF1518 0.82 -0.8 113,818 - GFF1518 0.82 -0.3 113,818 - GFF1518 0.82 +1.4 113,818 - GFF1518 0.82 +0.5 113,956 + -0.4 114,103 + GFF1519 0.20 +0.7 114,103 + GFF1519 0.20 +0.1 114,202 + GFF1519 0.30 -1.7 114,203 - GFF1519 0.30 +1.6 114,203 - GFF1519 0.30 -0.5 114,203 - GFF1519 0.30 -1.4 114,313 + GFF1519 0.41 +0.1 114,313 + GFF1519 0.41 +0.6 114,313 + GFF1519 0.41 -1.0 114,476 - GFF1519 0.58 +0.3 114,709 + GFF1519 0.81 +0.7 114,709 + GFF1519 0.81 -0.7 114,710 - GFF1519 0.81 +0.6 114,884 + +0.0 114,884 + -0.7 114,885 - -0.2 114,997 - +0.1 115,004 + +0.1 115,005 - -1.2 115,005 - -0.3 115,097 + +0.2 115,205 + GFF1520 0.19 -0.6 115,235 + GFF1520 0.23 +0.9 115,235 + GFF1520 0.23 +1.2 115,235 + GFF1520 0.23 +0.7 115,236 - GFF1520 0.23 -1.3 115,236 - GFF1520 0.23 +2.2 115,425 - GFF1520 0.47 -0.8 115,425 - GFF1520 0.47 -0.7 115,554 - GFF1520 0.63 -0.3 115,595 + GFF1520 0.69 +1.1 115,595 + GFF1520 0.69 -0.8 115,595 + GFF1520 0.69 -1.3 115,595 + GFF1520 0.69 +0.7 115,595 + GFF1520 0.69 -0.4 115,595 + GFF1520 0.69 -0.7 115,595 + GFF1520 0.69 +0.0 115,595 + GFF1520 0.69 -0.6 115,595 + GFF1520 0.69 -1.7 115,596 - GFF1520 0.69 -0.2 115,596 - GFF1520 0.69 -0.1 115,596 - GFF1520 0.69 -0.7 115,596 - GFF1520 0.69 -1.3 115,596 - GFF1520 0.69 -0.0 115,596 - GFF1520 0.69 -0.9 115,822 - -0.4 115,822 - +0.5
Or see this region's nucleotide sequence