Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1485 and GFF1486 are separated by 159 nucleotides GFF1486 and GFF1487 overlap by 4 nucleotides GFF1487 and GFF1488 are separated by 39 nucleotides
GFF1485 - Transcriptional regulator, AraC family, at 77,628 to 78,449
GFF1485
GFF1486 - Tetrapartite efflux system, inner membrane component FusBC-like, at 78,609 to 80,672
GFF1486
GFF1487 - Tetrapartite efflux system component, FusD-like, at 80,669 to 80,875
GFF1487
GFF1488 - Tetrapartite efflux system, membrane fusion component FusE-like, at 80,915 to 81,913
GFF1488
Position (kb) 78
79
80
81 Strain fitness (log2 ratio) -2
-1
0
1
2 at 77.684 kb on + strand at 77.684 kb on + strand at 77.684 kb on + strand at 77.686 kb on + strand at 77.686 kb on + strand at 77.687 kb on - strand at 77.687 kb on - strand at 77.961 kb on + strand, within GFF1485 at 78.076 kb on + strand, within GFF1485 at 78.077 kb on - strand, within GFF1485 at 78.187 kb on + strand, within GFF1485 at 78.188 kb on - strand, within GFF1485 at 78.332 kb on + strand, within GFF1485 at 78.597 kb on + strand at 78.597 kb on + strand at 78.598 kb on - strand at 78.684 kb on + strand at 78.684 kb on + strand at 78.684 kb on + strand at 78.685 kb on - strand at 78.685 kb on - strand at 78.685 kb on - strand at 78.738 kb on + strand at 78.738 kb on + strand at 78.738 kb on + strand at 78.739 kb on - strand at 78.739 kb on - strand at 78.903 kb on + strand, within GFF1486 at 78.904 kb on - strand, within GFF1486 at 78.936 kb on + strand, within GFF1486 at 78.936 kb on + strand, within GFF1486 at 78.937 kb on - strand, within GFF1486 at 78.937 kb on - strand, within GFF1486 at 78.937 kb on - strand, within GFF1486 at 79.726 kb on + strand, within GFF1486 at 79.726 kb on + strand, within GFF1486 at 79.726 kb on + strand, within GFF1486 at 79.727 kb on - strand, within GFF1486 at 80.013 kb on + strand, within GFF1486 at 80.013 kb on + strand, within GFF1486 at 80.013 kb on + strand, within GFF1486 at 80.215 kb on + strand, within GFF1486 at 80.215 kb on + strand, within GFF1486 at 80.216 kb on - strand, within GFF1486 at 80.216 kb on - strand, within GFF1486 at 80.287 kb on - strand, within GFF1486 at 80.696 kb on + strand, within GFF1487 at 80.696 kb on + strand, within GFF1487 at 80.696 kb on + strand, within GFF1487 at 80.696 kb on + strand, within GFF1487 at 80.696 kb on + strand, within GFF1487 at 80.696 kb on + strand, within GFF1487 at 80.697 kb on - strand, within GFF1487 at 80.697 kb on - strand, within GFF1487 at 80.822 kb on + strand, within GFF1487 at 80.822 kb on + strand, within GFF1487 at 81.510 kb on - strand, within GFF1488 at 81.561 kb on - strand, within GFF1488 at 81.561 kb on - strand, within GFF1488
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 77,684 + -0.2 77,684 + +1.1 77,684 + -0.2 77,686 + -0.7 77,686 + -0.0 77,687 - -0.1 77,687 - -0.1 77,961 + GFF1485 0.41 -1.0 78,076 + GFF1485 0.55 -0.2 78,077 - GFF1485 0.55 +1.8 78,187 + GFF1485 0.68 -0.1 78,188 - GFF1485 0.68 +0.3 78,332 + GFF1485 0.86 +0.2 78,597 + -0.3 78,597 + +0.1 78,598 - -0.0 78,684 + -0.1 78,684 + +0.3 78,684 + +1.1 78,685 - +0.6 78,685 - +0.4 78,685 - +0.2 78,738 + -0.1 78,738 + +1.3 78,738 + +0.3 78,739 - +0.3 78,739 - -0.0 78,903 + GFF1486 0.14 +0.5 78,904 - GFF1486 0.14 -0.1 78,936 + GFF1486 0.16 -0.0 78,936 + GFF1486 0.16 +0.2 78,937 - GFF1486 0.16 +2.0 78,937 - GFF1486 0.16 +0.2 78,937 - GFF1486 0.16 +1.2 79,726 + GFF1486 0.54 +1.1 79,726 + GFF1486 0.54 +0.7 79,726 + GFF1486 0.54 -0.6 79,727 - GFF1486 0.54 -0.3 80,013 + GFF1486 0.68 +0.7 80,013 + GFF1486 0.68 +0.0 80,013 + GFF1486 0.68 -0.3 80,215 + GFF1486 0.78 -0.6 80,215 + GFF1486 0.78 -0.9 80,216 - GFF1486 0.78 +0.9 80,216 - GFF1486 0.78 -0.6 80,287 - GFF1486 0.81 +0.6 80,696 + GFF1487 0.13 +0.2 80,696 + GFF1487 0.13 +1.1 80,696 + GFF1487 0.13 +0.4 80,696 + GFF1487 0.13 -0.4 80,696 + GFF1487 0.13 -2.2 80,696 + GFF1487 0.13 +0.7 80,697 - GFF1487 0.14 +0.1 80,697 - GFF1487 0.14 -1.3 80,822 + GFF1487 0.74 -1.0 80,822 + GFF1487 0.74 -0.3 81,510 - GFF1488 0.60 -0.0 81,561 - GFF1488 0.65 -0.1 81,561 - GFF1488 0.65 -0.2
Or see this region's nucleotide sequence