Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1459 and GFF1460 are separated by 114 nucleotides GFF1460 and GFF1461 are separated by 2 nucleotides GFF1461 and GFF1462 overlap by 4 nucleotides
GFF1459 - Glutathione S-transferase (EC 2.5.1.18), at 51,081 to 51,731
GFF1459
GFF1460 - Enoyl-CoA hydratase/isomerase, at 51,846 to 52,592
GFF1460
GFF1461 - Enoyl-CoA hydratase (EC 4.2.1.17), at 52,595 to 53,401
GFF1461
GFF1462 - L-carnitine dehydratase/bile acid-inducible protein F, at 53,398 to 54,600
GFF1462
Position (kb) 52
53
54 Strain fitness (log2 ratio) -2
-1
0
1 at 52.199 kb on - strand, within GFF1460 at 52.297 kb on + strand, within GFF1460 at 52.298 kb on - strand, within GFF1460 at 52.298 kb on - strand, within GFF1460 at 52.405 kb on + strand, within GFF1460 at 52.421 kb on - strand, within GFF1460 at 52.421 kb on - strand, within GFF1460 at 52.653 kb on + strand at 52.653 kb on + strand at 52.791 kb on + strand, within GFF1461 at 52.792 kb on - strand, within GFF1461 at 52.792 kb on - strand, within GFF1461 at 52.792 kb on - strand, within GFF1461 at 52.947 kb on + strand, within GFF1461 at 52.948 kb on - strand, within GFF1461 at 52.979 kb on + strand, within GFF1461 at 53.130 kb on + strand, within GFF1461 at 53.130 kb on + strand, within GFF1461 at 53.131 kb on - strand, within GFF1461 at 53.131 kb on - strand, within GFF1461 at 53.131 kb on - strand, within GFF1461 at 53.510 kb on + strand at 53.645 kb on + strand, within GFF1462 at 53.777 kb on + strand, within GFF1462 at 53.777 kb on + strand, within GFF1462 at 53.778 kb on - strand, within GFF1462 at 53.837 kb on + strand, within GFF1462 at 53.837 kb on + strand, within GFF1462 at 53.838 kb on - strand, within GFF1462 at 53.838 kb on - strand, within GFF1462 at 53.838 kb on - strand, within GFF1462 at 53.838 kb on - strand, within GFF1462 at 53.838 kb on - strand, within GFF1462 at 53.932 kb on + strand, within GFF1462 at 53.952 kb on - strand, within GFF1462 at 54.270 kb on - strand, within GFF1462
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 52,199 - GFF1460 0.47 +0.8 52,297 + GFF1460 0.60 -0.6 52,298 - GFF1460 0.61 +0.1 52,298 - GFF1460 0.61 -0.3 52,405 + GFF1460 0.75 -0.0 52,421 - GFF1460 0.77 -1.0 52,421 - GFF1460 0.77 +0.8 52,653 + -1.2 52,653 + +1.2 52,791 + GFF1461 0.24 +0.3 52,792 - GFF1461 0.24 -1.0 52,792 - GFF1461 0.24 -0.4 52,792 - GFF1461 0.24 -0.3 52,947 + GFF1461 0.44 -0.7 52,948 - GFF1461 0.44 +0.3 52,979 + GFF1461 0.48 +0.5 53,130 + GFF1461 0.66 -0.5 53,130 + GFF1461 0.66 -0.1 53,131 - GFF1461 0.66 -1.8 53,131 - GFF1461 0.66 -0.6 53,131 - GFF1461 0.66 -0.8 53,510 + -0.8 53,645 + GFF1462 0.21 -0.7 53,777 + GFF1462 0.32 -0.7 53,777 + GFF1462 0.32 -0.2 53,778 - GFF1462 0.32 +0.1 53,837 + GFF1462 0.36 -0.2 53,837 + GFF1462 0.36 +0.4 53,838 - GFF1462 0.37 -1.2 53,838 - GFF1462 0.37 +0.7 53,838 - GFF1462 0.37 +0.1 53,838 - GFF1462 0.37 -0.0 53,838 - GFF1462 0.37 +0.6 53,932 + GFF1462 0.44 +0.7 53,952 - GFF1462 0.46 -1.1 54,270 - GFF1462 0.72 +1.2
Or see this region's nucleotide sequence