Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1427 and GFF1428 overlap by 4 nucleotides GFF1428 and GFF1429 are separated by 126 nucleotides GFF1429 and GFF1430 are separated by 116 nucleotides
GFF1427 - Mandelate racemase (EC 5.1.2.2), at 17,712 to 18,788
GFF1427
GFF1428 - no description, at 18,785 to 18,940
GFF1428
GFF1429 - no description, at 19,067 to 19,438
GFF1429
GFF1430 - hypothetical protein, at 19,555 to 21,840
GFF1430
Position (kb) 19
20 Strain fitness (log2 ratio) -2
-1
0
1
2 at 18.436 kb on + strand, within GFF1427 at 18.600 kb on - strand, within GFF1427 at 18.970 kb on + strand at 18.971 kb on - strand at 18.971 kb on - strand at 18.971 kb on - strand at 19.222 kb on - strand, within GFF1429 at 19.222 kb on - strand, within GFF1429 at 19.305 kb on + strand, within GFF1429 at 19.305 kb on + strand, within GFF1429 at 19.608 kb on + strand at 19.609 kb on - strand at 19.609 kb on - strand at 19.609 kb on - strand at 19.698 kb on - strand at 19.698 kb on - strand at 19.698 kb on - strand at 19.850 kb on + strand, within GFF1430 at 19.851 kb on - strand, within GFF1430 at 19.851 kb on - strand, within GFF1430 at 19.856 kb on + strand, within GFF1430 at 19.935 kb on - strand, within GFF1430 at 19.960 kb on + strand, within GFF1430 at 19.978 kb on + strand, within GFF1430 at 20.073 kb on + strand, within GFF1430 at 20.074 kb on - strand, within GFF1430 at 20.074 kb on - strand, within GFF1430 at 20.075 kb on + strand, within GFF1430 at 20.075 kb on + strand, within GFF1430 at 20.076 kb on - strand, within GFF1430 at 20.076 kb on - strand, within GFF1430 at 20.321 kb on + strand, within GFF1430 at 20.322 kb on - strand, within GFF1430 at 20.357 kb on + strand, within GFF1430 at 20.357 kb on + strand, within GFF1430 at 20.358 kb on - strand, within GFF1430 at 20.364 kb on - strand, within GFF1430 at 20.364 kb on - strand, within GFF1430 at 20.414 kb on + strand, within GFF1430 at 20.415 kb on - strand, within GFF1430 at 20.415 kb on - strand, within GFF1430
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 18,436 + GFF1427 0.67 -0.3 18,600 - GFF1427 0.82 -1.7 18,970 + +0.3 18,971 - -0.5 18,971 - +1.0 18,971 - -1.2 19,222 - GFF1429 0.42 -1.3 19,222 - GFF1429 0.42 -0.2 19,305 + GFF1429 0.64 -0.7 19,305 + GFF1429 0.64 -0.7 19,608 + +0.7 19,609 - -0.1 19,609 - +0.1 19,609 - -1.8 19,698 - +0.3 19,698 - -0.5 19,698 - +0.1 19,850 + GFF1430 0.13 -0.2 19,851 - GFF1430 0.13 -0.1 19,851 - GFF1430 0.13 -0.0 19,856 + GFF1430 0.13 -0.2 19,935 - GFF1430 0.17 +2.7 19,960 + GFF1430 0.18 -0.7 19,978 + GFF1430 0.19 +0.6 20,073 + GFF1430 0.23 -0.2 20,074 - GFF1430 0.23 +0.3 20,074 - GFF1430 0.23 -0.9 20,075 + GFF1430 0.23 -1.1 20,075 + GFF1430 0.23 +0.1 20,076 - GFF1430 0.23 +1.0 20,076 - GFF1430 0.23 -0.1 20,321 + GFF1430 0.34 +0.2 20,322 - GFF1430 0.34 +0.1 20,357 + GFF1430 0.35 -0.2 20,357 + GFF1430 0.35 -0.0 20,358 - GFF1430 0.35 +0.7 20,364 - GFF1430 0.35 -0.5 20,364 - GFF1430 0.35 -1.2 20,414 + GFF1430 0.38 -0.3 20,415 - GFF1430 0.38 -0.4 20,415 - GFF1430 0.38 -1.9
Or see this region's nucleotide sequence