Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1424 and GFF1425 overlap by 1 nucleotides GFF1425 and GFF1426 are separated by 10 nucleotides GFF1426 and GFF1427 are separated by 7 nucleotides
GFF1424 - Phenylacetate ABC transporter, permease protein 2, at 15,293 to 16,225
GFF1424
GFF1425 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 16,225 to 16,974
GFF1425
GFF1426 - Phenylacetate ABC transporter, ATP-binding protein 2, at 16,985 to 17,704
GFF1426
GFF1427 - Mandelate racemase (EC 5.1.2.2), at 17,712 to 18,788
GFF1427
Position (kb)
16
17
18 Strain fitness (log2 ratio)
-1
0
1
2 at 16.125 kb on - strand, within GFF1424 at 16.125 kb on - strand, within GFF1424 at 16.235 kb on + strand at 16.236 kb on - strand at 16.236 kb on - strand at 16.988 kb on + strand at 17.351 kb on + strand, within GFF1426 at 17.352 kb on - strand, within GFF1426 at 17.352 kb on - strand, within GFF1426 at 17.352 kb on - strand, within GFF1426 at 17.352 kb on - strand, within GFF1426 at 17.612 kb on + strand, within GFF1426 at 17.612 kb on + strand, within GFF1426 at 17.612 kb on + strand, within GFF1426 at 17.613 kb on - strand, within GFF1426 at 17.690 kb on + strand at 17.690 kb on + strand at 17.822 kb on - strand, within GFF1427 at 17.859 kb on + strand, within GFF1427 at 17.859 kb on + strand, within GFF1427 at 17.860 kb on - strand, within GFF1427 at 17.860 kb on - strand, within GFF1427 at 17.860 kb on - strand, within GFF1427 at 17.872 kb on - strand, within GFF1427 at 17.967 kb on + strand, within GFF1427 at 17.968 kb on - strand, within GFF1427 at 18.436 kb on + strand, within GFF1427 at 18.600 kb on - strand, within GFF1427
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 16,125 - GFF1424 0.89 -1.0 16,125 - GFF1424 0.89 +1.0 16,235 + +0.7 16,236 - -0.0 16,236 - +0.0 16,988 + +0.2 17,351 + GFF1426 0.51 -0.0 17,352 - GFF1426 0.51 +0.6 17,352 - GFF1426 0.51 +1.4 17,352 - GFF1426 0.51 +0.9 17,352 - GFF1426 0.51 -0.8 17,612 + GFF1426 0.87 +0.8 17,612 + GFF1426 0.87 +1.9 17,612 + GFF1426 0.87 -0.1 17,613 - GFF1426 0.87 +0.3 17,690 + -1.3 17,690 + -0.9 17,822 - GFF1427 0.10 +0.5 17,859 + GFF1427 0.14 -1.2 17,859 + GFF1427 0.14 +0.1 17,860 - GFF1427 0.14 +1.7 17,860 - GFF1427 0.14 +0.7 17,860 - GFF1427 0.14 -0.9 17,872 - GFF1427 0.15 -0.7 17,967 + GFF1427 0.24 -0.8 17,968 - GFF1427 0.24 -0.5 18,436 + GFF1427 0.67 -0.3 18,600 - GFF1427 0.82 -1.7
Or see this region's nucleotide sequence