Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1351 and GFF1352 are separated by 47 nucleotides GFF1352 and GFF1353 are separated by 110 nucleotides
GFF1351 - Acyl-CoA dehydrogenase, at 693,686 to 695,326
GFF1351
GFF1352 - Probable glutathione s-transferase protein (EC 2.5.1.18), at 695,374 to 696,312
GFF1352
GFF1353 - Short-chain dehydrogenase/reductase SDR, at 696,423 to 697,328
GFF1353
Position (kb) 695
696
697 Strain fitness (log2 ratio) -1
0
1
2 at 694.902 kb on - strand, within GFF1351 at 694.940 kb on + strand, within GFF1351 at 694.940 kb on + strand, within GFF1351 at 694.941 kb on - strand, within GFF1351 at 695.398 kb on + strand at 695.398 kb on + strand at 695.398 kb on + strand at 695.398 kb on + strand at 695.399 kb on - strand at 695.399 kb on - strand at 695.399 kb on - strand at 695.399 kb on - strand at 695.560 kb on + strand, within GFF1352 at 695.560 kb on + strand, within GFF1352 at 695.560 kb on + strand, within GFF1352 at 695.560 kb on + strand, within GFF1352 at 695.560 kb on + strand, within GFF1352 at 695.560 kb on + strand, within GFF1352 at 695.561 kb on - strand, within GFF1352 at 695.561 kb on - strand, within GFF1352 at 695.561 kb on - strand, within GFF1352 at 695.561 kb on - strand, within GFF1352 at 695.561 kb on - strand, within GFF1352 at 695.561 kb on - strand, within GFF1352 at 695.561 kb on - strand, within GFF1352 at 695.561 kb on - strand, within GFF1352 at 695.836 kb on + strand, within GFF1352 at 695.837 kb on - strand, within GFF1352 at 695.837 kb on - strand, within GFF1352 at 695.846 kb on - strand, within GFF1352 at 695.846 kb on - strand, within GFF1352 at 695.846 kb on - strand, within GFF1352 at 695.926 kb on + strand, within GFF1352 at 695.927 kb on - strand, within GFF1352 at 695.942 kb on - strand, within GFF1352 at 696.223 kb on + strand at 696.224 kb on - strand at 696.287 kb on - strand at 696.367 kb on + strand at 696.375 kb on + strand at 696.376 kb on - strand at 696.376 kb on - strand at 696.840 kb on + strand, within GFF1353 at 696.841 kb on - strand, within GFF1353 at 696.906 kb on + strand, within GFF1353 at 696.906 kb on + strand, within GFF1353 at 696.906 kb on + strand, within GFF1353 at 696.907 kb on - strand, within GFF1353 at 696.907 kb on - strand, within GFF1353 at 696.907 kb on - strand, within GFF1353 at 696.907 kb on - strand, within GFF1353 at 696.907 kb on - strand, within GFF1353 at 696.951 kb on + strand, within GFF1353 at 696.951 kb on + strand, within GFF1353 at 696.952 kb on - strand, within GFF1353 at 696.952 kb on - strand, within GFF1353 at 697.005 kb on + strand, within GFF1353 at 697.049 kb on + strand, within GFF1353 at 697.049 kb on + strand, within GFF1353 at 697.049 kb on + strand, within GFF1353 at 697.188 kb on + strand, within GFF1353 at 697.188 kb on + strand, within GFF1353 at 697.189 kb on - strand, within GFF1353 at 697.189 kb on - strand, within GFF1353 at 697.189 kb on - strand, within GFF1353 at 697.189 kb on - strand, within GFF1353
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 694,902 - GFF1351 0.74 -0.4 694,940 + GFF1351 0.76 -0.7 694,940 + GFF1351 0.76 -0.5 694,941 - GFF1351 0.76 -0.6 695,398 + -0.2 695,398 + +1.3 695,398 + -0.7 695,398 + +0.1 695,399 - -0.1 695,399 - -0.2 695,399 - -0.1 695,399 - -0.5 695,560 + GFF1352 0.20 +0.1 695,560 + GFF1352 0.20 +0.6 695,560 + GFF1352 0.20 +0.7 695,560 + GFF1352 0.20 -0.4 695,560 + GFF1352 0.20 -0.7 695,560 + GFF1352 0.20 -0.2 695,561 - GFF1352 0.20 +0.0 695,561 - GFF1352 0.20 +0.3 695,561 - GFF1352 0.20 +1.1 695,561 - GFF1352 0.20 +0.3 695,561 - GFF1352 0.20 -0.2 695,561 - GFF1352 0.20 -0.4 695,561 - GFF1352 0.20 -0.2 695,561 - GFF1352 0.20 -0.1 695,836 + GFF1352 0.49 +0.3 695,837 - GFF1352 0.49 -0.4 695,837 - GFF1352 0.49 -0.1 695,846 - GFF1352 0.50 -0.7 695,846 - GFF1352 0.50 -0.0 695,846 - GFF1352 0.50 -0.8 695,926 + GFF1352 0.59 -0.4 695,927 - GFF1352 0.59 -0.5 695,942 - GFF1352 0.60 -0.1 696,223 + -0.9 696,224 - +2.3 696,287 - +2.0 696,367 + -0.3 696,375 + +0.2 696,376 - +0.0 696,376 - -0.6 696,840 + GFF1353 0.46 +0.1 696,841 - GFF1353 0.46 +1.2 696,906 + GFF1353 0.53 +0.1 696,906 + GFF1353 0.53 -1.2 696,906 + GFF1353 0.53 -1.0 696,907 - GFF1353 0.53 -0.1 696,907 - GFF1353 0.53 +0.6 696,907 - GFF1353 0.53 +0.5 696,907 - GFF1353 0.53 +1.0 696,907 - GFF1353 0.53 +0.3 696,951 + GFF1353 0.58 +0.3 696,951 + GFF1353 0.58 +1.0 696,952 - GFF1353 0.58 -0.5 696,952 - GFF1353 0.58 +0.7 697,005 + GFF1353 0.64 +1.5 697,049 + GFF1353 0.69 -0.1 697,049 + GFF1353 0.69 -0.1 697,049 + GFF1353 0.69 +0.4 697,188 + GFF1353 0.84 -0.0 697,188 + GFF1353 0.84 -1.5 697,189 - GFF1353 0.85 -1.0 697,189 - GFF1353 0.85 -0.4 697,189 - GFF1353 0.85 +1.3 697,189 - GFF1353 0.85 -0.6
Or see this region's nucleotide sequence