Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1326 and GFF1327 are separated by 139 nucleotides GFF1327 and GFF1328 overlap by 4 nucleotides GFF1328 and GFF1329 are separated by 10 nucleotides GFF1329 and GFF1330 overlap by 4 nucleotides
GFF1326 - Peptidyl-prolyl cis-trans isomerase (EC 5.2.1.8), at 666,570 to 667,355
GFF1326
GFF1327 - Cell division protein BolA, at 667,495 to 667,788
GFF1327
GFF1328 - Intracellular septation protein IspA, at 667,785 to 668,327
GFF1328
GFF1329 - Peptide-methionine (R)-S-oxide reductase MsrB (EC 1.8.4.12), at 668,338 to 668,754
GFF1329
GFF1330 - UPF0061 protein YdiU, at 668,751 to 670,232
GFF1330
Position (kb)
668
669 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 667.364 kb on + strand at 667.365 kb on - strand at 667.391 kb on + strand at 667.391 kb on + strand at 667.391 kb on + strand at 667.391 kb on + strand at 667.391 kb on + strand at 667.392 kb on - strand at 667.392 kb on - strand at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.556 kb on + strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.557 kb on - strand, within GFF1327 at 667.767 kb on + strand at 667.768 kb on - strand at 667.773 kb on + strand at 667.774 kb on - strand at 667.774 kb on - strand at 667.812 kb on - strand at 667.828 kb on + strand at 667.828 kb on + strand at 667.828 kb on + strand at 667.828 kb on + strand at 667.828 kb on + strand at 667.828 kb on + strand at 667.828 kb on + strand at 667.828 kb on + strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand at 667.829 kb on - strand at 668.384 kb on + strand, within GFF1329 at 668.384 kb on + strand, within GFF1329 at 668.384 kb on + strand, within GFF1329 at 668.385 kb on - strand, within GFF1329 at 668.385 kb on - strand, within GFF1329 at 668.385 kb on - strand, within GFF1329 at 668.385 kb on - strand, within GFF1329 at 668.385 kb on - strand, within GFF1329 at 668.385 kb on - strand, within GFF1329 at 668.603 kb on + strand, within GFF1329 at 668.603 kb on + strand, within GFF1329 at 668.603 kb on + strand, within GFF1329 at 668.603 kb on + strand, within GFF1329 at 668.603 kb on + strand, within GFF1329 at 668.603 kb on + strand, within GFF1329 at 668.604 kb on - strand, within GFF1329 at 668.604 kb on - strand, within GFF1329 at 668.604 kb on - strand, within GFF1329 at 668.604 kb on - strand, within GFF1329 at 668.604 kb on - strand, within GFF1329 at 668.604 kb on - strand, within GFF1329 at 668.604 kb on - strand, within GFF1329 at 668.604 kb on - strand, within GFF1329 at 668.604 kb on - strand, within GFF1329 at 668.630 kb on + strand, within GFF1329 at 668.630 kb on + strand, within GFF1329 at 668.630 kb on + strand, within GFF1329 at 668.630 kb on + strand, within GFF1329 at 668.630 kb on + strand, within GFF1329 at 668.631 kb on - strand, within GFF1329 at 668.631 kb on - strand, within GFF1329 at 668.631 kb on - strand, within GFF1329 at 668.740 kb on + strand at 668.806 kb on + strand at 668.806 kb on + strand at 668.806 kb on + strand at 668.806 kb on + strand at 668.806 kb on + strand at 668.806 kb on + strand at 668.806 kb on + strand at 668.807 kb on - strand at 668.807 kb on - strand at 668.831 kb on - strand at 669.079 kb on + strand, within GFF1330 at 669.079 kb on + strand, within GFF1330 at 669.079 kb on + strand, within GFF1330 at 669.079 kb on + strand, within GFF1330 at 669.079 kb on + strand, within GFF1330 at 669.079 kb on + strand, within GFF1330 at 669.079 kb on + strand, within GFF1330 at 669.079 kb on + strand, within GFF1330 at 669.080 kb on - strand, within GFF1330 at 669.080 kb on - strand, within GFF1330 at 669.080 kb on - strand, within GFF1330 at 669.080 kb on - strand, within GFF1330 at 669.080 kb on - strand, within GFF1330 at 669.080 kb on - strand, within GFF1330 at 669.109 kb on + strand, within GFF1330 at 669.110 kb on - strand, within GFF1330 at 669.110 kb on - strand, within GFF1330 at 669.110 kb on - strand, within GFF1330 at 669.238 kb on + strand, within GFF1330 at 669.238 kb on + strand, within GFF1330 at 669.238 kb on + strand, within GFF1330 at 669.239 kb on - strand, within GFF1330 at 669.239 kb on - strand, within GFF1330 at 669.239 kb on - strand, within GFF1330 at 669.239 kb on - strand, within GFF1330 at 669.239 kb on - strand, within GFF1330 at 669.239 kb on - strand, within GFF1330 at 669.262 kb on - strand, within GFF1330 at 669.262 kb on - strand, within GFF1330 at 669.262 kb on - strand, within GFF1330 at 669.319 kb on + strand, within GFF1330 at 669.319 kb on + strand, within GFF1330 at 669.320 kb on - strand, within GFF1330 at 669.349 kb on + strand, within GFF1330 at 669.350 kb on - strand, within GFF1330 at 669.547 kb on + strand, within GFF1330 at 669.547 kb on + strand, within GFF1330 at 669.547 kb on + strand, within GFF1330 at 669.607 kb on + strand, within GFF1330 at 669.607 kb on + strand, within GFF1330 at 669.607 kb on + strand, within GFF1330 at 669.607 kb on + strand, within GFF1330 at 669.607 kb on + strand, within GFF1330 at 669.607 kb on + strand, within GFF1330 at 669.607 kb on + strand, within GFF1330 at 669.607 kb on + strand, within GFF1330 at 669.608 kb on - strand, within GFF1330 at 669.608 kb on - strand, within GFF1330 at 669.608 kb on - strand, within GFF1330 at 669.608 kb on - strand, within GFF1330 at 669.608 kb on - strand, within GFF1330 at 669.608 kb on - strand, within GFF1330 at 669.608 kb on - strand, within GFF1330
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 667,364 + -0.7 667,365 - -1.5 667,391 + -0.8 667,391 + +1.3 667,391 + -0.2 667,391 + -0.6 667,391 + +0.3 667,392 - -0.6 667,392 - -0.5 667,556 + GFF1327 0.21 -1.2 667,556 + GFF1327 0.21 -0.3 667,556 + GFF1327 0.21 -0.3 667,556 + GFF1327 0.21 -0.5 667,556 + GFF1327 0.21 +0.6 667,556 + GFF1327 0.21 -0.7 667,556 + GFF1327 0.21 -0.1 667,556 + GFF1327 0.21 -0.2 667,556 + GFF1327 0.21 -0.5 667,556 + GFF1327 0.21 -0.2 667,556 + GFF1327 0.21 +0.7 667,556 + GFF1327 0.21 +0.2 667,556 + GFF1327 0.21 +1.6 667,556 + GFF1327 0.21 +1.1 667,556 + GFF1327 0.21 +0.2 667,556 + GFF1327 0.21 -1.5 667,556 + GFF1327 0.21 -0.6 667,556 + GFF1327 0.21 -1.3 667,557 - GFF1327 0.21 -0.3 667,557 - GFF1327 0.21 -0.2 667,557 - GFF1327 0.21 -0.3 667,557 - GFF1327 0.21 -2.7 667,557 - GFF1327 0.21 +0.1 667,557 - GFF1327 0.21 +0.4 667,557 - GFF1327 0.21 +1.3 667,557 - GFF1327 0.21 -0.2 667,557 - GFF1327 0.21 -0.3 667,557 - GFF1327 0.21 +0.0 667,557 - GFF1327 0.21 +0.3 667,557 - GFF1327 0.21 +0.1 667,557 - GFF1327 0.21 +0.5 667,557 - GFF1327 0.21 -0.4 667,557 - GFF1327 0.21 -0.8 667,767 + -0.5 667,768 - +0.1 667,773 + +0.1 667,774 - -0.5 667,774 - -0.1 667,812 - +0.1 667,828 + -1.4 667,828 + -1.2 667,828 + +0.9 667,828 + -0.5 667,828 + -0.7 667,828 + +0.9 667,828 + -0.5 667,828 + +1.2 667,829 - +1.1 667,829 - +1.8 667,829 - +0.0 667,829 - -1.9 667,829 - -0.1 667,829 - -0.2 667,829 - -0.8 667,829 - +1.7 667,829 - -0.9 667,829 - -0.5 667,829 - -0.5 667,829 - +0.7 668,384 + GFF1329 0.11 +1.0 668,384 + GFF1329 0.11 -2.0 668,384 + GFF1329 0.11 -0.5 668,385 - GFF1329 0.11 +0.3 668,385 - GFF1329 0.11 -0.4 668,385 - GFF1329 0.11 -0.4 668,385 - GFF1329 0.11 -0.8 668,385 - GFF1329 0.11 +0.1 668,385 - GFF1329 0.11 -0.3 668,603 + GFF1329 0.64 +0.4 668,603 + GFF1329 0.64 +0.3 668,603 + GFF1329 0.64 -0.3 668,603 + GFF1329 0.64 -1.4 668,603 + GFF1329 0.64 -1.3 668,603 + GFF1329 0.64 -0.0 668,604 - GFF1329 0.64 +0.6 668,604 - GFF1329 0.64 -1.0 668,604 - GFF1329 0.64 +0.3 668,604 - GFF1329 0.64 -1.3 668,604 - GFF1329 0.64 -1.3 668,604 - GFF1329 0.64 -0.0 668,604 - GFF1329 0.64 -0.6 668,604 - GFF1329 0.64 -0.1 668,604 - GFF1329 0.64 -0.6 668,630 + GFF1329 0.70 +0.3 668,630 + GFF1329 0.70 +0.0 668,630 + GFF1329 0.70 -0.2 668,630 + GFF1329 0.70 -1.4 668,630 + GFF1329 0.70 -1.2 668,631 - GFF1329 0.70 -0.6 668,631 - GFF1329 0.70 -0.0 668,631 - GFF1329 0.70 +0.7 668,740 + +0.9 668,806 + +0.8 668,806 + +0.8 668,806 + -1.1 668,806 + -1.5 668,806 + +1.0 668,806 + -0.6 668,806 + +0.5 668,807 - -0.9 668,807 - -1.9 668,831 - -1.0 669,079 + GFF1330 0.22 -0.2 669,079 + GFF1330 0.22 -0.7 669,079 + GFF1330 0.22 -1.5 669,079 + GFF1330 0.22 +0.2 669,079 + GFF1330 0.22 -0.6 669,079 + GFF1330 0.22 -0.7 669,079 + GFF1330 0.22 -1.0 669,079 + GFF1330 0.22 +0.2 669,080 - GFF1330 0.22 -0.8 669,080 - GFF1330 0.22 -0.2 669,080 - GFF1330 0.22 -0.7 669,080 - GFF1330 0.22 +0.7 669,080 - GFF1330 0.22 -0.0 669,080 - GFF1330 0.22 -0.3 669,109 + GFF1330 0.24 -1.2 669,110 - GFF1330 0.24 +0.1 669,110 - GFF1330 0.24 -0.1 669,110 - GFF1330 0.24 -0.2 669,238 + GFF1330 0.33 +0.2 669,238 + GFF1330 0.33 +0.3 669,238 + GFF1330 0.33 -0.0 669,239 - GFF1330 0.33 -0.1 669,239 - GFF1330 0.33 -0.3 669,239 - GFF1330 0.33 -0.1 669,239 - GFF1330 0.33 +0.1 669,239 - GFF1330 0.33 -1.3 669,239 - GFF1330 0.33 -1.1 669,262 - GFF1330 0.34 +0.2 669,262 - GFF1330 0.34 +0.6 669,262 - GFF1330 0.34 -1.4 669,319 + GFF1330 0.38 -0.7 669,319 + GFF1330 0.38 -0.4 669,320 - GFF1330 0.38 +0.4 669,349 + GFF1330 0.40 -0.6 669,350 - GFF1330 0.40 -1.3 669,547 + GFF1330 0.54 -1.3 669,547 + GFF1330 0.54 -0.5 669,547 + GFF1330 0.54 +1.1 669,607 + GFF1330 0.58 -0.1 669,607 + GFF1330 0.58 +0.2 669,607 + GFF1330 0.58 -0.5 669,607 + GFF1330 0.58 +0.0 669,607 + GFF1330 0.58 +0.3 669,607 + GFF1330 0.58 +0.1 669,607 + GFF1330 0.58 -0.4 669,607 + GFF1330 0.58 +0.4 669,608 - GFF1330 0.58 +0.7 669,608 - GFF1330 0.58 +0.6 669,608 - GFF1330 0.58 +0.8 669,608 - GFF1330 0.58 +1.6 669,608 - GFF1330 0.58 -0.6 669,608 - GFF1330 0.58 -0.6 669,608 - GFF1330 0.58 -0.3
Or see this region's nucleotide sequence