Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1188 and GFF1189 are separated by 21 nucleotides GFF1189 and GFF1190 overlap by 4 nucleotides GFF1190 and GFF1191 are separated by 927 nucleotides
GFF1188 - Thiazole synthase (EC 2.8.1.10), at 505,726 to 506,541
GFF1188
GFF1189 - Sulfur carrier protein ThiS, at 506,563 to 506,760
GFF1189
GFF1190 - Glycine oxidase ThiO (EC 1.4.3.19), at 506,757 to 507,848
GFF1190
GFF1191 - Predicted hydroxymethylpyrimidine transporter CytX, at 508,776 to 510,110
GFF1191
Position (kb)
506
507
508 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 506.118 kb on - strand, within GFF1188 at 506.118 kb on - strand, within GFF1188 at 506.118 kb on - strand, within GFF1188 at 506.118 kb on - strand, within GFF1188 at 506.196 kb on - strand, within GFF1188 at 506.196 kb on - strand, within GFF1188 at 506.196 kb on - strand, within GFF1188 at 506.628 kb on - strand, within GFF1189 at 506.998 kb on + strand, within GFF1190 at 506.999 kb on - strand, within GFF1190 at 506.999 kb on - strand, within GFF1190 at 506.999 kb on - strand, within GFF1190 at 506.999 kb on - strand, within GFF1190 at 507.109 kb on + strand, within GFF1190 at 507.109 kb on + strand, within GFF1190 at 507.110 kb on - strand, within GFF1190 at 508.007 kb on + strand at 508.008 kb on - strand at 508.098 kb on - strand at 508.290 kb on - strand at 508.715 kb on + strand at 508.715 kb on + strand at 508.715 kb on + strand at 508.716 kb on - strand at 508.716 kb on - strand at 508.716 kb on - strand at 508.716 kb on - strand at 508.716 kb on - strand at 508.716 kb on - strand at 508.716 kb on - strand at 508.716 kb on - strand at 508.716 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 506,118 - GFF1188 0.48 +0.3 506,118 - GFF1188 0.48 -1.3 506,118 - GFF1188 0.48 -0.3 506,118 - GFF1188 0.48 -0.3 506,196 - GFF1188 0.58 +0.0 506,196 - GFF1188 0.58 +2.7 506,196 - GFF1188 0.58 -0.1 506,628 - GFF1189 0.33 -2.9 506,998 + GFF1190 0.22 -1.9 506,999 - GFF1190 0.22 -0.4 506,999 - GFF1190 0.22 -0.9 506,999 - GFF1190 0.22 +0.5 506,999 - GFF1190 0.22 -1.5 507,109 + GFF1190 0.32 -1.7 507,109 + GFF1190 0.32 +0.4 507,110 - GFF1190 0.32 +0.7 508,007 + -1.0 508,008 - -0.3 508,098 - -0.7 508,290 - -0.6 508,715 + +1.7 508,715 + +0.4 508,715 + -1.3 508,716 - -0.0 508,716 - +0.7 508,716 - +0.1 508,716 - +1.7 508,716 - +0.3 508,716 - +0.2 508,716 - -1.2 508,716 - -0.1 508,716 - -1.3
Or see this region's nucleotide sequence