Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1119 and GFF1120 are separated by 65 nucleotides GFF1120 and GFF1121 are separated by 22 nucleotides GFF1121 and GFF1122 are separated by 4 nucleotides GFF1122 and GFF1123 overlap by 4 nucleotides
GFF1119 - Ribonuclease HI (EC 3.1.26.4), at 436,138 to 436,620
GFF1119
GFF1120 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 436,686 to 437,141
GFF1120
GFF1121 - Molybdopterin molybdenumtransferase (EC 2.10.1.1), at 437,164 to 438,474
GFF1121
GFF1122 - Molybdenum cofactor guanylyltransferase (EC 2.7.7.77), at 438,479 to 439,138
GFF1122
GFF1123 - GTP 3',8-cyclase (EC 4.1.99.22), at 439,135 to 440,292
GFF1123
Position (kb)
437
438
439 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 436.189 kb on + strand, within GFF1119 at 436.189 kb on + strand, within GFF1119 at 436.189 kb on + strand, within GFF1119 at 436.189 kb on + strand, within GFF1119 at 436.190 kb on - strand, within GFF1119 at 436.190 kb on - strand, within GFF1119 at 436.190 kb on - strand, within GFF1119 at 436.191 kb on + strand, within GFF1119 at 436.369 kb on + strand, within GFF1119 at 436.370 kb on - strand, within GFF1119 at 436.370 kb on - strand, within GFF1119 at 436.370 kb on - strand, within GFF1119 at 436.370 kb on - strand, within GFF1119 at 436.384 kb on + strand, within GFF1119 at 436.385 kb on - strand, within GFF1119 at 436.385 kb on - strand, within GFF1119 at 436.412 kb on + strand, within GFF1119 at 436.719 kb on + strand at 436.719 kb on + strand at 436.719 kb on + strand at 436.968 kb on + strand, within GFF1120 at 436.968 kb on + strand, within GFF1120 at 436.968 kb on + strand, within GFF1120 at 436.968 kb on + strand, within GFF1120 at 436.968 kb on + strand, within GFF1120 at 436.968 kb on + strand, within GFF1120 at 436.968 kb on + strand, within GFF1120 at 436.969 kb on - strand, within GFF1120 at 436.969 kb on - strand, within GFF1120 at 437.139 kb on + strand at 437.165 kb on + strand at 437.165 kb on + strand at 437.165 kb on + strand at 437.349 kb on - strand, within GFF1121 at 437.737 kb on + strand, within GFF1121 at 437.737 kb on + strand, within GFF1121 at 437.737 kb on + strand, within GFF1121 at 437.737 kb on + strand, within GFF1121 at 437.737 kb on + strand, within GFF1121 at 437.738 kb on - strand, within GFF1121 at 437.738 kb on - strand, within GFF1121 at 437.796 kb on - strand, within GFF1121 at 437.796 kb on - strand, within GFF1121 at 437.810 kb on + strand, within GFF1121 at 437.810 kb on + strand, within GFF1121 at 437.811 kb on - strand, within GFF1121 at 437.811 kb on - strand, within GFF1121 at 437.870 kb on + strand, within GFF1121 at 437.870 kb on + strand, within GFF1121 at 437.870 kb on + strand, within GFF1121 at 437.870 kb on + strand, within GFF1121 at 437.871 kb on - strand, within GFF1121 at 437.871 kb on - strand, within GFF1121 at 437.871 kb on - strand, within GFF1121 at 438.082 kb on + strand, within GFF1121 at 438.082 kb on + strand, within GFF1121 at 438.082 kb on + strand, within GFF1121 at 438.083 kb on - strand, within GFF1121 at 438.083 kb on - strand, within GFF1121 at 438.255 kb on - strand, within GFF1121 at 438.255 kb on - strand, within GFF1121 at 438.435 kb on - strand at 438.564 kb on - strand, within GFF1122 at 438.882 kb on + strand, within GFF1122 at 438.883 kb on - strand, within GFF1122 at 438.883 kb on - strand, within GFF1122 at 438.927 kb on + strand, within GFF1122 at 438.927 kb on + strand, within GFF1122 at 438.927 kb on + strand, within GFF1122 at 438.927 kb on + strand, within GFF1122 at 438.927 kb on + strand, within GFF1122 at 438.927 kb on + strand, within GFF1122 at 438.927 kb on + strand, within GFF1122 at 438.928 kb on - strand, within GFF1122 at 438.928 kb on - strand, within GFF1122 at 438.928 kb on - strand, within GFF1122 at 438.928 kb on - strand, within GFF1122 at 438.928 kb on - strand, within GFF1122 at 438.928 kb on - strand, within GFF1122 at 438.928 kb on - strand, within GFF1122 at 438.989 kb on + strand, within GFF1122 at 438.989 kb on + strand, within GFF1122 at 438.989 kb on + strand, within GFF1122 at 438.990 kb on - strand, within GFF1122 at 438.990 kb on - strand, within GFF1122 at 439.148 kb on + strand at 439.148 kb on + strand at 439.148 kb on + strand at 439.148 kb on + strand at 439.148 kb on + strand at 439.148 kb on + strand at 439.148 kb on + strand at 439.148 kb on + strand at 439.148 kb on + strand at 439.148 kb on + strand at 439.148 kb on + strand at 439.148 kb on + strand at 439.148 kb on + strand at 439.148 kb on + strand at 439.148 kb on + strand at 439.148 kb on + strand at 439.148 kb on + strand at 439.149 kb on - strand at 439.149 kb on - strand at 439.149 kb on - strand at 439.149 kb on - strand at 439.149 kb on - strand at 439.149 kb on - strand at 439.149 kb on - strand at 439.229 kb on + strand at 439.229 kb on + strand at 439.230 kb on - strand at 439.346 kb on + strand, within GFF1123 at 439.346 kb on + strand, within GFF1123 at 439.346 kb on + strand, within GFF1123 at 439.347 kb on - strand, within GFF1123 at 439.347 kb on - strand, within GFF1123 at 439.347 kb on - strand, within GFF1123 at 439.347 kb on - strand, within GFF1123 at 439.445 kb on + strand, within GFF1123 at 439.445 kb on + strand, within GFF1123 at 439.445 kb on + strand, within GFF1123 at 439.446 kb on - strand, within GFF1123 at 439.446 kb on - strand, within GFF1123 at 439.446 kb on - strand, within GFF1123 at 439.446 kb on - strand, within GFF1123
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 436,189 + GFF1119 0.11 -1.0 436,189 + GFF1119 0.11 +2.2 436,189 + GFF1119 0.11 -1.4 436,189 + GFF1119 0.11 +0.2 436,190 - GFF1119 0.11 -0.8 436,190 - GFF1119 0.11 +0.1 436,190 - GFF1119 0.11 +0.2 436,191 + GFF1119 0.11 +0.5 436,369 + GFF1119 0.48 -1.3 436,370 - GFF1119 0.48 -1.6 436,370 - GFF1119 0.48 -1.2 436,370 - GFF1119 0.48 +1.3 436,370 - GFF1119 0.48 -0.8 436,384 + GFF1119 0.51 +0.0 436,385 - GFF1119 0.51 -0.2 436,385 - GFF1119 0.51 -1.2 436,412 + GFF1119 0.57 +0.8 436,719 + +0.3 436,719 + -1.2 436,719 + -0.4 436,968 + GFF1120 0.62 -0.6 436,968 + GFF1120 0.62 -0.2 436,968 + GFF1120 0.62 -0.4 436,968 + GFF1120 0.62 -0.1 436,968 + GFF1120 0.62 -2.4 436,968 + GFF1120 0.62 -1.2 436,968 + GFF1120 0.62 -1.2 436,969 - GFF1120 0.62 +1.3 436,969 - GFF1120 0.62 +1.1 437,139 + -0.3 437,165 + -0.4 437,165 + -1.0 437,165 + -0.4 437,349 - GFF1121 0.14 -0.2 437,737 + GFF1121 0.44 -1.2 437,737 + GFF1121 0.44 -0.9 437,737 + GFF1121 0.44 +0.0 437,737 + GFF1121 0.44 -1.5 437,737 + GFF1121 0.44 -0.8 437,738 - GFF1121 0.44 -0.8 437,738 - GFF1121 0.44 -0.6 437,796 - GFF1121 0.48 +0.1 437,796 - GFF1121 0.48 -2.4 437,810 + GFF1121 0.49 -0.2 437,810 + GFF1121 0.49 -0.0 437,811 - GFF1121 0.49 +0.6 437,811 - GFF1121 0.49 +0.2 437,870 + GFF1121 0.54 -0.8 437,870 + GFF1121 0.54 +0.2 437,870 + GFF1121 0.54 -0.7 437,870 + GFF1121 0.54 -2.1 437,871 - GFF1121 0.54 +0.2 437,871 - GFF1121 0.54 -0.4 437,871 - GFF1121 0.54 -1.3 438,082 + GFF1121 0.70 -0.4 438,082 + GFF1121 0.70 -0.6 438,082 + GFF1121 0.70 -0.7 438,083 - GFF1121 0.70 +0.2 438,083 - GFF1121 0.70 -0.4 438,255 - GFF1121 0.83 -0.7 438,255 - GFF1121 0.83 -0.3 438,435 - +0.1 438,564 - GFF1122 0.13 -1.0 438,882 + GFF1122 0.61 -0.7 438,883 - GFF1122 0.61 -0.1 438,883 - GFF1122 0.61 -0.3 438,927 + GFF1122 0.68 -1.0 438,927 + GFF1122 0.68 +0.1 438,927 + GFF1122 0.68 +0.8 438,927 + GFF1122 0.68 +0.6 438,927 + GFF1122 0.68 -0.0 438,927 + GFF1122 0.68 -0.0 438,927 + GFF1122 0.68 -0.0 438,928 - GFF1122 0.68 -0.2 438,928 - GFF1122 0.68 -0.7 438,928 - GFF1122 0.68 +0.3 438,928 - GFF1122 0.68 +0.1 438,928 - GFF1122 0.68 -0.7 438,928 - GFF1122 0.68 +0.4 438,928 - GFF1122 0.68 +0.2 438,989 + GFF1122 0.77 -2.0 438,989 + GFF1122 0.77 -0.1 438,989 + GFF1122 0.77 +0.1 438,990 - GFF1122 0.77 -0.4 438,990 - GFF1122 0.77 +0.1 439,148 + -1.4 439,148 + +0.3 439,148 + +0.7 439,148 + -0.7 439,148 + +0.3 439,148 + +0.2 439,148 + -0.1 439,148 + -1.1 439,148 + +0.3 439,148 + +0.4 439,148 + -0.8 439,148 + -0.2 439,148 + -1.0 439,148 + -0.0 439,148 + -0.3 439,148 + -0.6 439,148 + -1.6 439,149 - +1.6 439,149 - -1.4 439,149 - -0.4 439,149 - +0.3 439,149 - +1.0 439,149 - -0.2 439,149 - -0.1 439,229 + +1.3 439,229 + -1.0 439,230 - -0.3 439,346 + GFF1123 0.18 +1.6 439,346 + GFF1123 0.18 -0.3 439,346 + GFF1123 0.18 -1.0 439,347 - GFF1123 0.18 -1.5 439,347 - GFF1123 0.18 -0.1 439,347 - GFF1123 0.18 -1.7 439,347 - GFF1123 0.18 -0.1 439,445 + GFF1123 0.27 +0.3 439,445 + GFF1123 0.27 -0.3 439,445 + GFF1123 0.27 -0.8 439,446 - GFF1123 0.27 -0.5 439,446 - GFF1123 0.27 +0.0 439,446 - GFF1123 0.27 +0.0 439,446 - GFF1123 0.27 -0.2
Or see this region's nucleotide sequence