Experiment: M9 with Supernatant from Cyptococcus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1064 and GFF1065 are separated by 59 nucleotides GFF1065 and GFF1066 overlap by 4 nucleotides GFF1066 and GFF1067 are separated by 11 nucleotides GFF1067 and GFF1068 are separated by 2 nucleotides
GFF1064 - Transcriptional regulator, GntR family, at 377,419 to 378,153
GFF1064
GFF1065 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 378,213 to 379,046
GFF1065
GFF1066 - Aspartate aminotransferase (EC 2.6.1.1), at 379,043 to 380,218
GFF1066
GFF1067 - Hydrolase, alpha/beta fold family, at 380,230 to 381,111
GFF1067
GFF1068 - Beta-ureidopropionase (EC 3.5.1.6), at 381,114 to 382,403
GFF1068
Position (kb) 379
380
381 Strain fitness (log2 ratio) -2
-1
0
1 at 378.242 kb on - strand at 378.375 kb on + strand, within GFF1065 at 378.375 kb on + strand, within GFF1065 at 378.376 kb on - strand, within GFF1065 at 378.558 kb on + strand, within GFF1065 at 378.558 kb on + strand, within GFF1065 at 378.559 kb on - strand, within GFF1065 at 378.583 kb on - strand, within GFF1065 at 378.660 kb on + strand, within GFF1065 at 378.661 kb on - strand, within GFF1065 at 378.661 kb on - strand, within GFF1065 at 378.859 kb on - strand, within GFF1065 at 378.859 kb on - strand, within GFF1065 at 378.933 kb on + strand, within GFF1065 at 378.934 kb on - strand, within GFF1065 at 379.238 kb on + strand, within GFF1066 at 379.239 kb on - strand, within GFF1066 at 379.239 kb on - strand, within GFF1066 at 379.239 kb on - strand, within GFF1066 at 379.239 kb on - strand, within GFF1066 at 379.538 kb on + strand, within GFF1066 at 379.538 kb on + strand, within GFF1066 at 379.538 kb on + strand, within GFF1066 at 379.539 kb on - strand, within GFF1066 at 379.539 kb on - strand, within GFF1066 at 379.571 kb on - strand, within GFF1066 at 379.574 kb on + strand, within GFF1066 at 379.574 kb on + strand, within GFF1066 at 379.574 kb on + strand, within GFF1066 at 379.575 kb on - strand, within GFF1066 at 379.575 kb on - strand, within GFF1066 at 379.575 kb on - strand, within GFF1066 at 379.649 kb on + strand, within GFF1066 at 379.650 kb on - strand, within GFF1066 at 379.795 kb on - strand, within GFF1066 at 379.795 kb on - strand, within GFF1066 at 380.078 kb on + strand, within GFF1066 at 380.078 kb on + strand, within GFF1066 at 380.078 kb on + strand, within GFF1066 at 380.266 kb on + strand at 380.267 kb on - strand at 380.269 kb on + strand at 380.269 kb on + strand at 380.597 kb on - strand, within GFF1067 at 380.913 kb on - strand, within GFF1067 at 380.913 kb on - strand, within GFF1067 at 381.144 kb on - strand at 381.144 kb on - strand at 381.144 kb on - strand at 381.144 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Supernatant from Cyptococcus remove 378,242 - +0.1 378,375 + GFF1065 0.19 -0.6 378,375 + GFF1065 0.19 +0.0 378,376 - GFF1065 0.20 +0.4 378,558 + GFF1065 0.41 -0.6 378,558 + GFF1065 0.41 -1.8 378,559 - GFF1065 0.41 -0.4 378,583 - GFF1065 0.44 +0.2 378,660 + GFF1065 0.54 +0.0 378,661 - GFF1065 0.54 -0.0 378,661 - GFF1065 0.54 -0.4 378,859 - GFF1065 0.77 -0.8 378,859 - GFF1065 0.77 +0.6 378,933 + GFF1065 0.86 -1.2 378,934 - GFF1065 0.86 -0.6 379,238 + GFF1066 0.17 +0.1 379,239 - GFF1066 0.17 -0.9 379,239 - GFF1066 0.17 -0.4 379,239 - GFF1066 0.17 -1.8 379,239 - GFF1066 0.17 -0.2 379,538 + GFF1066 0.42 -0.4 379,538 + GFF1066 0.42 +0.7 379,538 + GFF1066 0.42 -0.6 379,539 - GFF1066 0.42 -0.1 379,539 - GFF1066 0.42 -0.1 379,571 - GFF1066 0.45 +0.2 379,574 + GFF1066 0.45 +1.1 379,574 + GFF1066 0.45 +1.1 379,574 + GFF1066 0.45 +0.4 379,575 - GFF1066 0.45 -1.6 379,575 - GFF1066 0.45 -1.0 379,575 - GFF1066 0.45 +0.2 379,649 + GFF1066 0.52 -0.2 379,650 - GFF1066 0.52 -0.4 379,795 - GFF1066 0.64 -1.2 379,795 - GFF1066 0.64 +1.3 380,078 + GFF1066 0.88 +0.7 380,078 + GFF1066 0.88 -0.6 380,078 + GFF1066 0.88 +0.1 380,266 + +0.3 380,267 - -0.3 380,269 + -1.1 380,269 + +0.3 380,597 - GFF1067 0.42 +1.0 380,913 - GFF1067 0.77 -1.1 380,913 - GFF1067 0.77 +0.8 381,144 - -0.4 381,144 - -0.4 381,144 - -1.6 381,144 - -0.8
Or see this region's nucleotide sequence